Different Modes of Interaction in Cyanobacterial Complexes of Plastocyanin and Cytochrome f
| العنوان: | Different Modes of Interaction in Cyanobacterial Complexes of Plastocyanin and Cytochrome f |
|---|---|
| المؤلفون: | Miguel A. De la Rosa, Irene Díaz-Moreno, Peter B. Crowley, Marcellus Ubbink, Antonio Díaz-Quintana |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | American Chemical Society, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Models, Molecular, Nostoc, Cytochrome, Static Electricity, Cytochrome f, Cyanobacteria, Photosynthesis, Photochemistry, Biochemistry, Electron transfer, Plastocyanin, Nuclear Magnetic Resonance, Biomolecular, biology, Cytochrome b6f complex, Osmolar Concentration, Nuclear magnetic resonance spectroscopy, biology.organism_classification, Binding constant, Cytochromes f, Multiprotein Complexes, biology.protein, Biophysics, Hydrophobic and Hydrophilic Interactions, Cyanobacterium, Protein Binding |
| الوصف: | 8 páginas, 6 figuras, 1 tabla The highly efficient electron-transfer chain in photosynthesis demonstrates a remarkable variation among organisms in the type of interactions between the soluble electron-transfer protein plastocyanin and it partner cytochrome f. The complex from the cyanobacterium Nostoc sp. PCC 7119 was studied using nuclear magnetic resonance spectroscopy and compared to that of the cyanobacterium Phormidium laminosum. In both systems, the main site of interaction on plastocyanin is the hydrophobic patch. However, the interaction in the Nostoc complex is highly dependent on electrostatics, contrary to that of Phormidium, resulting in a binding constant that is an order of magnitude larger at low ionic strength for the Nostoc complex. Studies of the mixed complexes show that these differences in interactions are mainly attributable to the surface properties of the plastocyanins. This work was supported by grants from the Improving Human Potential Program of the European Commission (contract no. HPRNCT- 1999-00095), by the Spanish Ministry of Science and Technology (Grant BMC2003-00458), and by The Netherlands Organisation for Scientific Research (Grant 700.52.425). I.D.-M. was also the recipient of an individual fellowship from the Spanish Ministry of Education, Culture, and Sport(AP2000-2937). |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49d14e54225fccf1f276d9384a219f1eTest http://hdl.handle.net/10261/33655Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....49d14e54225fccf1f276d9384a219f1e |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |