دورية أكاديمية
Biophysical Characterization of Pro-apoptotic BimBH3 Peptides Reveals an Unexpected Capacity for Self-Association
| العنوان: | Biophysical Characterization of Pro-apoptotic BimBH3 Peptides Reveals an Unexpected Capacity for Self-Association |
|---|---|
| المؤلفون: | Assafa, Tufa E., Nandi, Sukhendu, Śmiłowicz, Dariusz, Galazzo, Laura, Teucher, Markus, Elsner, Christina Mareike, Pütz, Stefanie, Bleicken, Stephanie, Robin, Adeline Y., Westphal, Dana, Uson, Isabel, Stoll, Raphael, Czabotar, Peter E., Metzler-Nolte, Nils, Bordignon, Enrica |
| المصدر: | ISSN: 0969-2126 ; Structure, vol. 29, no. 2 (2021) p. 114-124.e3. |
| سنة النشر: | 2021 |
| المجموعة: | Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: | BH3, Bcl-xL, Bim peptides, DEER, EPR, X-ray, Apoptosis |
| الوصف: | Bcl-2 proteins orchestrate the mitochondrial pathway of apoptosis, pivotal for cell death. Yet, the structural details of the conformational changes of pro- and antiapoptotic proteins and their interactions remain unclear. Pulse dipolar spectroscopy (double electron-electron resonance [DEER], also known as PELDOR) in combination with spin-labeled apoptotic Bcl-2 proteins unveils conformational changes and interactions of each protein player via detection of intra- and inter-protein distances. Here, we present the synthesis and characterization of pro-apoptotic BimBH3 peptides of different lengths carrying cysteines for labeling with nitroxide or gadolinium spin probes. We show by DEER that the length of the peptides modulates their homo-interactions in the absence of other Bcl-2 proteins and solve by X-ray crystallography the structure of a BimBH3 tetramer, revealing the molecular details of the inter-peptide interactions. Finally, we prove that using orthogonal labels and three-channel DEER we can disentangle the Bim-Bim, Bcl-xL-Bcl-xL, and Bim-Bcl-xL interactions in a simplified interactome . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/32966763; https://archive-ouverte.unige.ch/unige:173950Test; unige:173950 |
| الإتاحة: | https://doi.org/10.1016/j.str.2020.09.002Test https://archive-ouverte.unige.ch/unige:173950Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.181399AD |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |