A Practical Guide to Rodent Islet Isolation and Assessment
| العنوان: | A Practical Guide to Rodent Islet Isolation and Assessment |
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| المؤلفون: | Jeffrey D. Carter, Kathryn L. Corbin, Runpei Wu, Stacey B. Dula, Craig S. Nunemaker |
| المصدر: | Biological Procedures Biological Procedures Online, Vol 11, Iss 1, Pp 3-31 (2009) Biological Procedures Online, Vol 11, Iss 1, p 9021 (2009) |
| بيانات النشر: | BioMed Central, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | endocrine system, insulin, endocrine system diseases, medicine.medical_treatment, beta-cell, 030209 endocrinology & metabolism, Review, Biology, General Biochemistry, Genetics and Molecular Biology, necrosis, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, islet isolation, rat, lcsh:QH301-705.5, mouse, 030304 developmental biology, 0303 health sciences, geography, lcsh:R5-920, geography.geographical_feature_category, pancreatic islets, calcium, islet, Biochemistry, Genetics and Molecular Biology(all), Pancreatic islets, Insulin, apoptosis, rodent, Islet, cytokines, Cell biology, Transplantation, collagenase, beta cells, medicine.anatomical_structure, lcsh:Biology (General), Immunology, Beta cell, islets of Langerhans, Pancreas, lcsh:Medicine (General), isolation, Function (biology), transplantation |
| الوصف: | Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture conditions. In addition, this paper reviews commonly used techniques for assessing islet viability and function, including visual assessment, fluorescent markers of cell death, glucose-stimulated insulin secretion, and intracellular calcium measurements. A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. The purpose of this review is to serve as a resource and foundation for successfully procuring and purifying high-quality islets for research purposes. |
| اللغة: | English |
| تدمد: | 1480-9222 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55adc6d19e11689e2fbfb12c43403e88Test http://europepmc.org/articles/PMC3056052Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....55adc6d19e11689e2fbfb12c43403e88 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14809222 |
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