تقرير
Neurotensin orchestrates valence assignment in the amygdala
| العنوان: | Neurotensin orchestrates valence assignment in the amygdala |
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| المؤلفون: | Li, Hao, Namburi, Praneeth, Olson, Jacob M., Borio, Matilde, Lemieux, Mackenzie E., Beyeler, Anna, Calhoon, Gwendolyn G., Hitora-Imamura, Natsuko, Coley, Austin A., Libster, Avraham, Bal, Aneesh, Jin, Xin, Wang, Huan, Jia, Caroline, Choudhury, Sourav R., Shi, Xi, Felix-Ortiz, Ada C., de la Fuente, Veronica, Barth, Vanessa P., King, Hunter O., Izadmehr, Ehsan M., Revanna, Jasmin S., Batra, Kanha, Fischer, Kyle B., Keyes, Laurel R., Padilla-Coreano, Nancy, Siciliano, Cody A., Mccullough, Kenneth M., Wichmann, Romy, Ressler, Kerry J., Fiete, Ila R., Zhang, Feng, Li, Yulong, Tye, Kay M. |
| المساهمون: | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale (U1215 Inserm - UB), Université de Bordeaux (UB)-Institut François Magendie-Institut National de la Santé et de la Recherche Médicale (INSERM), Salk Institute for Biological Studies, Howard Hughes Medical Institute, JPB Foundation, Alfred P. Sloan Foundation, New York Stem Cell Foundation, Klingenstein Third Generation Foundation, Kavli Foundation, Dolby Family Ventures, Pioneer Fund, Uehara Memorial Foundation |
| المصدر: | https://hal.science/hal-04062021Test ; 2023. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2023 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Protein expression, Protein function, Signal transduction, Whole cell patch clamp, Amygdala, Physiology, Reward, Basolateral Nuclear Complex, Calcium, Neurotensin, Neuropeptide, Glutamic acid, Adult, Animal cell, Animal experiment, Animal tissue, Article, Associative learning, Basolateral amygdala, Brain region, C57BL 6 mouse, Cell culture, Cohort analysis, Confocal microscopy, Controlled study, CRISPR-CAS9 system, Female, Human, In situ hybridization, In vivo study |
| الوصف: | The ability to associate temporally segregated information and assign positive or negative valence to environmental cues is paramount for survival. Studies have shown that different basolateral amygdala (BLA) projections are potentiated following reward or punishment learning1–7. However, we do not yet understand how valence specific information is routed to the BLA neurons with the appropriate downstream projections. Nor do we understand how to reconcile the subsecond timescales of synaptic plasticity8–11 with the longer timescales separating the predictive cues from their outcomes. Here, we demonstrate that neurotensin (NT) neurons in the paraventricular nucleus of the thalamus (PVT) projecting to the BLA (PVT-BLA:NT) mediate valence assignment by exerting concentration-dependent modulation in BLA during associative learning. We found that optogenetic activation of the PVT-BLA:NT projection promotes reward learning, while PVT-BLA projection-specific Nt gene knockout augments punishment learning. Using genetically encoded calcium and NT sensors, we further revealed that both calcium dynamics within the PVT-BLA:NT projection and NT concentrations in the BLA are enhanced after reward learning and reduced after punishment learning. Finally, we showed that CRISPR-mediated knockout of the Nt gene in the PVT-BLA pathway blunts BLA neural dynamics and attenuates the preference to active behavioral strategies to reward and punishment predictive cues. Taken together, we have identified NT as a neuropeptide that signals valence in the BLA, and showed that NT is a critical neuromodulator that orchestrates positive and negative valence assignment in amygdala neurons by extending valence-specific plasticity to behaviorally-relevant timescales. |
| نوع الوثيقة: | report |
| اللغة: | English |
| العلاقة: | hal-04062021; https://hal.science/hal-04062021Test; https://hal.science/hal-04062021/documentTest; https://hal.science/hal-04062021/file/MAGENDIE_Nature_2022_Li.pdfTest |
| DOI: | 10.1038/s41586-022-04964-y |
| الإتاحة: | https://doi.org/10.1038/s41586-022-04964-yTest https://hal.science/hal-04062021Test https://hal.science/hal-04062021/documentTest https://hal.science/hal-04062021/file/MAGENDIE_Nature_2022_Li.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F5136450 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41586-022-04964-y |
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