Loss of XRN4 Function Can Trigger Cosuppression in a Sequence-Dependent Manner
| العنوان: | Loss of XRN4 Function Can Trigger Cosuppression in a Sequence-Dependent Manner |
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| المؤلفون: | Chieko Nanba, Maki Kondo, Yuichiro Watanabe, Atsushi Takeda, Miyuki Saito, Makoto Hayashi, Mikio Nishimura |
| المصدر: | Plant and Cell Physiology. 53:1310-1321 |
| بيانات النشر: | Oxford University Press (OUP), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Cosuppression, Physiology, Recombinant Fusion Proteins, Transgene, Green Fluorescent Proteins, Mutant, Arabidopsis, Plant Science, Genes, Plant, Chromosomes, Plant, Fluorescence, Green fluorescent protein, Transformation, Genetic, Oil body, Gene Expression Regulation, Plant, RNA, Messenger, Transgenes, Cloning, Molecular, Plant Proteins, Base Sequence, Arabidopsis Proteins, Chemistry, beta-Glucosidase, Endoplasmic reticulum, Seed Storage Proteins, Chromosome Mapping, Cell Biology, General Medicine, Plants, Genetically Modified, Fusion protein, Cell biology, Phenotype, Exoribonucleases, Mutation, Seeds, RNA Interference, Oleosin, Plasmids |
| الوصف: | OLE1 encodes an oleosin isoprotein, a major membrane protein of the lipid-reserve organelle in seeds known as the oil body. Transgenic Arabidopsis were generated to contain an artificial chimeric transgene composed of OLE1 and green fluorescent protein (GFP). Overexpression of the fusion protein allowed visualization of the oil body size and structure in living cells using fluorescence microscopy. Two mutants, xrn4-8(OleG) and xrn4-9(OleG), accumulating enlarged oil bodies with reduced GFP fluorescence were isolated from the mutagenized progeny of a transgenic plant. Both mutants contained a defect in EXORIBONUCLEASE4 (XRN4), a gene known to encode a ribonuclease that specifically degrades uncapped mRNAs. Transgene expression was silenced in these mutants, as demonstrated by the reduced levels of the transgene mRNA and its product, OLE1-GFP. XRN4 loss of function also triggered cosuppression, i.e. simultaneous reduction in expression of the transgene and an endogenous OLE1 gene that shared a region of identical sequence. The enlarged oil bodies exhibiting reduced GFP fluorescence were formed in the xrn4-8(OleG) and xrn4-9(OleG) mutants due to the reduction of the endogenous OLE1 and the transgene product, OLE1-GFP, respectively. Cosuppression triggered by the xrn4 mutation also occurs for other genes such as PYK10, which encodes an endoplasmic reticulum (ER) body-resident β-glucosidase. The overall results indicate that a loss of XRN4 function can potentially trigger the cosuppression in a sequence-dependent manner. |
| تدمد: | 1471-9053 0032-0781 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a6f9235af9e6079216443800f77dbcbTest https://doi.org/10.1093/pcp/pcs078Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1a6f9235af9e6079216443800f77dbcb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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