Microvirga pakistanensis sp. nov., a novel bacterium isolated from desert soil of Cholistan, Pakistan
| العنوان: | Microvirga pakistanensis sp. nov., a novel bacterium isolated from desert soil of Cholistan, Pakistan |
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| المؤلفون: | Arshia Amin, Wael N. Hozzein, Neeli Habib, Iftikhar Ahmed, Saira Abbas, Wen-Jun Li, Fariha Hasan, Min Xiao |
| المصدر: | Archives of Microbiology. 198:933-939 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | DNA, Bacterial, 0301 basic medicine, food.ingredient, Microvirga, Sodium Chloride, Biology, medicine.disease_cause, Biochemistry, Microbiology, Microvirga vignae, Soil, 03 medical and health sciences, food, RNA, Ribosomal, 16S, Botany, Genetics, medicine, Microvirga zambiensis, Pakistan, Microvirga flocculans, Molecular Biology, Phylogeny, Soil Microbiology, Base Composition, Base Sequence, Microvirga lupini, Strain (chemistry), Fatty Acids, Nucleic Acid Hybridization, Sequence Analysis, DNA, General Medicine, rpoB, 16S ribosomal RNA, Bacterial Typing Techniques, 030104 developmental biology, Desert Climate, Methylobacteriaceae |
| الوصف: | A Gram-negative, non-spore-forming, non-pigmented, strictly aerobic and non-motile short rod bacterium, designated NCCP-1258T, was isolated from Cholistan desert soil, Bahawalpur, Pakistan. Growth of strain NCCP-1258T was observed at pH range 6.5–9.5 (optimum 7.5–8.5) and temperature range 20–45 °C (optimum 40 °C), and it tolerated 0–2 % NaCl (optimum 0.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain NCCP-1258T belongs to genus Microvirga and is most closely related to Microvirgalotononidis (98.0 %), Microvirga vignae (97.4 %), Microvirga lupini (97.2 %), Microvirga zambiensis (97.2 %) and Microvirga flocculans (97.1 %). Analysis of the concatenated sequences of four housekeeping gene loci (dnaK, gyrB, recA and rpoB) also confirmed the placement of strain NCCP-1258T within the genus Microvirga. DNA–DNA relatedness values of NCCP-1258T with above-mentioned type strains were less than 42 %. The DNA G+C content of strain NCCP-1258T was 64.3 mol%. Chemotaxonomic data (predominant menaquinone system was Q-10; major fatty acids were C16:0, C18:1 ω7c and C19:0 cyclo ω8c; the polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidyl dimethyl ethanolamine and phosphatidyl ethanolamine) also supported the affiliation of strain NCCP-1258T to the genus Microvirga. On the basis of physiological and biochemical characteristics, phylogenetic analyses and DNA–DNA relatedness, strain NCCP-1258T can be distinguished from the closely related taxa and thus represents a novel species of the genus Microvirga, for which the name Microvirgapakistanensis sp. nov. is proposed with the type strain NCCP-1258T (=CGMCC 1.15074T = KCTC 42496T). |
| تدمد: | 1432-072X 0302-8933 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b1355e8c09eecb11199d8f54b433913Test https://doi.org/10.1007/s00203-016-1251-3Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6b1355e8c09eecb11199d8f54b433913 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432072X 03028933 |
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