دورية أكاديمية
TIMP-2 gene transfer by positively charged PEG-lated monosized polycationic carrier to smooth muscle cells
| العنوان: | TIMP-2 gene transfer by positively charged PEG-lated monosized polycationic carrier to smooth muscle cells |
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| المؤلفون: | Laçin, N., Utkan G., Kutsal, T., Dedeoglu, B.G., Yulug I.G., Pişkin, E. |
| المصدر: | Journal of Nanoparticle Research |
| سنة النشر: | 2012 |
| المجموعة: | Bilkent University: Institutional Repository |
| مصطلحات موضوعية: | Gene therapy, Nanomedicine, Nanoparticles, SMC, TIMP, Average size, Balloon angioplasty, Cardiac disease, Extracellular matrices, Extracellular matrix degradation, Matrix metalloproteinase-2, Matrix metalloproteinases, Metalloproteinases, Mono-sized, Nanomedicines, Nonviral vectors, Polyplexes, Positively charged, Potential values, Protein level, Restenosis, Smooth muscle cells, Therapeutic potentials, Tissue inhibitor, Western blotting, Blood vessels, Cells, Enzyme inhibition, Gene expression, Gene transfer |
| الوصف: | Remodeling of the extracellular matrix resulting from increased secretion of metalloproteinase enzymes is implicated in restenosis following balloon angioplasty. Matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases play an essential role in both normal and pathological extracellular matrix degradation. Tissue inhibitor of matrix metalloproteinase- 2 is the most extensively studied tissue inhibitor of metalloproteinases inmyocardial tissue in animalmodels and clinical examples of cardiac disease; therefore it is selected for this study. Gene transfer of tissue inhibitor of matrix metalloproteinase-2 may have a therapeutic potential by inhibition of matrix metalloproteinase activity. We have used PEG-lated nanoparticles poly(St/PEG-EEM/DMAPM) which were synthesized previously in our laboratory. The nanoparticles, with an average size of 77.6 ± 2.05 nm with a zeta potential of +64. 4 ± 1.14 mVand 201.9 ± 1.83 nmwith +54.2 ± 0.77 mV were used in the transfection studies. Zeta Potential values and size of polyplex were appropriate for an effective transfection. TIMP-2 expression was detected by western blotting. Increased protein level in smoothmuscle cells according to non-transfected smooth muscle cells confirms the successful delivery and expression of the tissue inhibitor of matrix metalloproteinase- 2 gene with the non-viral vector transfection approach. © Springer Science+Business Media B.V. 2012. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 13880764 |
| العلاقة: | http://dx.doi.org/10.1007/s11051-011-0694-3Test; http://hdl.handle.net/11693/21607Test |
| DOI: | 10.1007/s11051-011-0694-3 |
| الإتاحة: | https://doi.org/10.1007/s11051-011-0694-3Test http://hdl.handle.net/11693/21607Test |
| رقم الانضمام: | edsbas.ABF32835 |
| قاعدة البيانات: | BASE |
| تدمد: | 13880764 |
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| DOI: | 10.1007/s11051-011-0694-3 |