Analysis of HDAC6 and BAG3-aggresome pathways in African swine fever viral factory formation
| العنوان: | Analysis of HDAC6 and BAG3-aggresome pathways in African swine fever viral factory formation |
|---|---|
| المؤلفون: | Lucía Barrado-Gil, Raquel Muñoz-Moreno, Covadonga Alonso, Inmaculada Galindo |
| المصدر: | Viruses, Vol 7, Iss 4, Pp 1823-1831 (2015) Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Viruses INIA: Repositorio de Resultados de Investigación del INIA |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Aggresome, Swine, Dynein, lcsh:QR1-502, Biology, Virus Replication, African swine fever virus, Histone Deacetylases, lcsh:Microbiology, Cell Line, Virology, Viral factory, Animals, Gene silencing, Viral factories, Adaptor Proteins, Signal Transducing, BAG3, Communication, DNA virus, HDAC6, biology.organism_classification, Infectious Diseases, Viral replication, Host-Pathogen Interactions, Dynactin |
| الوصف: | African swine fever virus (ASFV) is a double-stranded DNA virus causing a hemorrhagic fever disease with high mortality rates and severe economic losses in pigs worldwide. ASFV replicates in perinuclear sites called viral factories (VFs) that are morphologically similar to cellular aggresomes. This fact raises the possibility that both VFs and aggresomes may be the same structure. However, little is known about the process involved in the formation of these viral replication platforms. In order to expand our knowledge on the assembly of ASFV replication sites, we have analyzed the involvement of both canonical aggresome pathways in the formation of ASFV VFs HDAC6 and BAG3. HDAC6 interacts with a component of the dynein motor complex (dynactin/p150Glued) and ubiquitinated proteins, transporting them to the microtubule-organizing center (MTOC) and leading to aggresome formation, while BAG3 is mediating the recruitment of non-ubiquitinated proteins through a similar mechanism. Tubacin-mediated HDAC6 inhibition and silencing of BAG3 pathways, individually or simultaneously, did not prevent ASFV VF formation. These findings show that HDAC6 and Bag3 are not required for VFs formation suggesting that aggresomes and VFs are not the same structures. However, alternative unexplored pathways may be involved in the formation of aggresomes. © 2015 by the authors; licensee MDPI, Basel, Switzerland. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7986f45db6f443327f26e6e0822244fcTest http://hdl.handle.net/10261/292701Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7986f45db6f443327f26e6e0822244fc |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |