A Novel Alphabaculovirus from the Soybean Looper, Chrysodeixis includens, that Produces Tetrahedral Occlusion Bodies and Encodes Two Copies of he65
| العنوان: | A Novel Alphabaculovirus from the Soybean Looper, Chrysodeixis includens, that Produces Tetrahedral Occlusion Bodies and Encodes Two Copies of he65 |
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| المؤلفون: | Robert L. Harrison, Daniel L. Rowley, Holly J. R. Popham |
| المصدر: | Viruses Viruses, Vol 11, Iss 7, p 579 (2019) Volume 11 Issue 7 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Chrysodeixis includens, 0301 basic medicine, Subfamily, food.ingredient, 030106 microbiology, lcsh:QR1-502, he65, Gene Dosage, Genome, Viral, Occlusion Bodies, Viral, Plusiinae, lef-12, Moths, lcsh:Microbiology, Article, Viral Proteins, 03 medical and health sciences, baculovirus, food, polyhedrin, Virology, Trichoplusia, Polyhedrin, Animals, Amino Acid Sequence, ORFS, soybean looper, Phylogeny, Genetics, biology, occlusion body, fungi, biology.organism_classification, Nucleopolyhedroviruses, DNA ligase 3, Open reading frame, Alphabaculovirus, 030104 developmental biology, Infectious Diseases, Larva, Soybeans, Sequence Alignment |
| الوصف: | Isolates of the alphabaculovirus species, Chrysodeixis includens nucleopolyhedrovirus, have been identified that produce polyhedral occlusion bodies and infect larvae of the soybean looper, Chrysodeixis includens. In this study, we report the discovery and characterization of a novel C. includens-infecting alphabaculovirus, Chrysodeixis includens nucleopolyhedrovirus #1 (ChinNPV#1), that produces tetrahedral occlusion bodies. In bioassays against C. includens larvae, ChinNPV #1 exhibited a degree of pathogenicity that was similar to that of other ChinNPV isolates, but killed larvae more slowly. The host range of ChinNPV#1 was found to be very narrow, with no indication of infection occurring in larvae of Trichoplusia ni and six other noctuid species. The ChinNPV#1 genome sequence was determined to be 130,540 bp, with 126 open reading frames (ORFs) annotated but containing no homologous repeat (hr) regions. Phylogenetic analysis placed ChinNPV#1 in a clade with other Group II alphabaculoviruses from hosts of lepidopteran subfamily Plusiinae, including Chrysodeixis chalcites nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus. A unique feature of the ChinNPV#1 genome was the presence of two full-length copies of the he65 ORF. The results indicate that ChinNPV#1 is related to, but distinct from, other ChinNPV isolates. |
| وصف الملف: | application/pdf |
| تدمد: | 1999-4915 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::551fa5bbceaceb87b4138c139e8a3c15Test https://doi.org/10.3390/v11070579Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....551fa5bbceaceb87b4138c139e8a3c15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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