دورية أكاديمية
Structural framework for DNA translocation via the viral portal protein
| العنوان: | Structural framework for DNA translocation via the viral portal protein |
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| المؤلفون: | Lebedev, Andrey A., Krause, Margret H., Isidro, Anabela L., Vagin, Alexei A., Orlova, Elena V., Turner, Joanne, Dodson, Eleanor J., Tavares, Paulo, Antson, Alfred A. |
| المساهمون: | University of York, Max Planck Institute for Molecular Genetics (MPIMG), Max-Planck-Gesellschaft, Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut Fédératif de Recherche 115 - Génomes, Transcriptomes, Protéomes (IFR 115), Centre National de la Recherche Scientifique (CNRS), Instituto de Technologia Quimica e Biologica, University of London |
| المصدر: | ISSN: 0261-4189. |
| بيانات النشر: | HAL CCSD EMBO Press |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | BACTERIOPHAGE SPP1, DNA TRANSLOCATION, MOLECULAR MOTOR, X-RAY CRYSTALLOGRAPHY, VIRAL PORTAL PROTEIN, [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | International audience ; Tailed bacteriophages and herpesviruses load their capsids with DNA through a tunnel formed by the portal protein assembly. Here we describe the X-ray structure of the bacteriophage SPP1 portal protein in its isolated 13-subunit form and the pseudoatomic structure of a 12-subunit assembly. The first defines the DNA-interacting segments ( tunnel loops) that pack tightly against each other forming the most constricted part of the tunnel; the second shows that the functional dodecameric state must induce variability in the loop positions. Structural observations together with geometrical constraints dictate that in the portal-DNA complex, the loops form an undulating belt that fits and tightly embraces the helical DNA, suggesting that DNA translocation is accompanied by a 'mexican wave' of positional and conformational changes propagating sequentially along this belt. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02661862; https://hal.inrae.fr/hal-02661862Test; PRODINRA: 147326; PUBMEDCENTRAL: PMC1847669; WOS: 000245465800022 |
| DOI: | 10.1038/sj.emboj.7601643 |
| الإتاحة: | https://doi.org/10.1038/sj.emboj.7601643Test https://hal.inrae.fr/hal-02661862Test |
| رقم الانضمام: | edsbas.D37F1DA2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/sj.emboj.7601643 |
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