Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
| العنوان: | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
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| المؤلفون: | Maite Muniesa, Elisenda Miró, William Calero-Cáceres, Ferran Navarro, Paula Espinal, Maryury Brown-Jaque, Thais Cornejo, Juan José González-López, Judith Rodríguez-Navarro, Juan Carlos Hurtado |
| المساهمون: | Universitat de Barcelona |
| المصدر: | INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname Dipòsit Digital de la UB Universidad de Barcelona Recercat. Dipósit de la Recerca de Catalunya |
| بيانات النشر: | ELSEVIER, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Klebsiella pneumoniae, Antibiotic resistance, Antibiotics, medicine.disease_cause, Bacteriophage, Feces, Bacteriophages, Pharmacology (medical), Child, Aged, 80 and over, Faeces, General Medicine, Horizontal gene transfer, Middle Aged, Bacteriòfags, Healthy Volunteers, Infectious Diseases, Child, Preschool, Female, Sample collection, Adult, Microbiology (medical), Adolescent, medicine.drug_class, 030106 microbiology, Antibiòtics, Biology, Real-Time Polymerase Chain Reaction, Microbiology, Young Adult, 03 medical and health sciences, Transduction, Drug Resistance, Bacterial, Escherichia coli, medicine, Humans, Gene, Aged, Resistència als medicaments, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, 030104 developmental biology, Genes, Bacterial, Drug resistance, DNA, Viral |
| الوصف: | Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group), bla(OXA-48), qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group), armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with bla(TEM) and bla(CTX-M-9 group) being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group) and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved. |
| وصف الملف: | application/pdf |
| تدمد: | 0924-8579 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b01640eda0b8c3afc449c05cc97b754Test https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=3839Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5b01640eda0b8c3afc449c05cc97b754 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09248579 |
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