التفاصيل البيبلوغرافية
| العنوان: |
The Role of Nigella sativa in improving the immune response of the African Catfish (Clarias gariepinus) to Aeromonas hydrophila Vaccine. |
| المؤلفون: |
Aly, Salah M.1 salahaly@hotmail.com, Ismail, Mona M.2, Fathi, Mohamed3, Zohairy, Mohamed A. Al4 |
| المصدر: |
Egyptian Journal of Aquatic Biology & Fisheries. Autumn2019, Vol. 23 Issue 4, p373-384. 12p. |
| مصطلحات موضوعية: |
*CLARIAS gariepinus, *BLACK cumin, *AEROMONAS hydrophila, *CATFISHES, *IMMUNE response |
| مستخلص: |
In the past few decades, the search for alternative strategies for disease management other than chemotherapeutants has been encouraged. Recently, disease prevention among fishes depends mainly on the use of vaccine and immunostimulants together with optimal husbandry condition. The present study examined the effects of Nigella sativa (NS) on the response of catfish (Clarias gariepinus to vaccination and/or challenge infection. For this purpose, four hundred catfish allocated into four groups with two feeding regimes (basal diet (G1 & G2) and diet supplemented with NS at dose level of 3 g/100 g diet (G3 & G4). The groups (G2 & G4) are intraperitoneally (IP) vaccinated with 0.1 ml formalin-killed Aeromonas hydrophila bacterin. The effects of NS in G3 and G4 are evaluated in comparison with other groups (G1 & G2) using hematological and biochemical parameters together with histopathological examinations. Six weeks after the start of experiment, the results revealed significant increase in erythrogram (RBCs, Hb and PCV%), Leucogram (total Differential leucocytic count), serum total protein and globulin in catfish supplemented with 3% NS. On the other hand, no significant changes in liver enzymes, urea, uric acid and creatinine among all experimental groups were noticed. The histopathology revealed proliferation of hematopoietic tissues and activation of melanomacrophages in both supplemented and vaccinated groups. Two weeks after challenge experiment, vaccinated groups (G2 and G4) showed significant increase in the antibody titer while treated groups supplemented with 3% NS showed significant increase in the phagocytic activity. After challenge, catfish vaccinated and supplemented with 3% NS showed highest (95%)relative level of protection (RLP) followed by vaccinated group (85%) then supplemented group(55%). Nigella sativa (3 g/100 g diet) enhance the immune response and resistance of catfish to A. hydrophila and consequently has a potential value for aquaculture. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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