التفاصيل البيبلوغرافية
| العنوان: |
Transcriptome analysis of ionic-liquid tolerant Bacillus amyloliquefaciens CMW1 and identification of a novel efflux pump. |
| المؤلفون: |
Kurata, Atsushi1 (AUTHOR), Aoki, Daichi1 (AUTHOR), Fukuta, Yoshihumi1 (AUTHOR), Kamimura, Taichi1 (AUTHOR), Onishi, Taiki1 (AUTHOR), Kishimoto, Noriaki1 (AUTHOR), Uegaki, Koichi1 (AUTHOR) |
| المصدر: |
Biotechnology & Biotechnological Equipment. 2021, Vol. 35 Issue 1, p445-452. 8p. |
| مصطلحات موضوعية: |
*BACILLUS amyloliquefaciens, *BACILLUS (Bacteria), *DNA microarrays, *TRANSCRIPTOMES, *GRAM-positive bacteria, *BIOCONVERSION, *CHEMICAL industry |
| مستخلص: |
Bacteria that exhibit ionic-liquid (IL) tolerance are useful in chemical industries using renewable carbon sources pre-treated by ILs to produce biofuels and fine chemicals. An IL, 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), has a remarkable ability to solubilize wood components, whereas [BMIM]Cl inhibits the growth of various bacterial hosts useful for bioconversion. We previously isolated a 10% [BMIM]Cl-tolerant bacterium Bacillus amyloliquefaciens CMW1. Here we report novel mechanisms of tolerance to [BMIM]Cl in strain CMW1 and a novel major facilitator superfamily transporter coded by an ionic-liquid tolerance (ILT) gene. First, using CMW1 cells grown in the presence or absence of 10% [BMIM]Cl, whole-transcriptome analysis and differentially expressed gene analysis were performed. Probable mechanisms of tolerance to [BMIM]Cl include the uptake of osmoprotectants from the culture medium toward CMW1 cells and the export of [BMIM] cations that accumulated in CMW1 cells. The finding represents a first step in elucidation of the mechanisms of IL resistance in Gram-positive bacteria. Second, we conferred tolerance to 5% [BMIM]Cl on [BMIM]Cl-susceptible Brevibacillus choshinensis using ILT gene. This finding provides a notable basis for engineering IL-tolerant bacterial hosts that are applicable for the effective and sustainable production of industrially important chemicals. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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