Genetic and functional diversity of Bacillus strains in the soils long-term irrigated with paper and pulp mill effluent
| العنوان: | Genetic and functional diversity of Bacillus strains in the soils long-term irrigated with paper and pulp mill effluent |
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| المؤلفون: | Dilip K. Arora, Anil Kumar Saxena, Subhash Yadav, Rajeev Kaushik |
| المصدر: | The Journal of General and Applied Microbiology. 57:183-195 |
| بيانات النشر: | Microbiology Research Foundation, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Paper, Irrigation, Veterinary medicine, Agricultural Irrigation, Molecular Sequence Data, India, Industrial Waste, Bacillus, Fresh Water, Waste Disposal, Fluid, Applied Microbiology and Biotechnology, Microbiology, Soil, Paenibacillus, Diversity index, RNA, Ribosomal, 16S, Botany, Cluster Analysis, Soil Pollutants, Bacillaceae, Effluent, Phylogeny, Soil Microbiology, Base Sequence, biology, business.industry, Genetic Variation, Paper mill, Sequence Analysis, DNA, Hydrogen-Ion Concentration, biology.organism_classification, Amplified Ribosomal DNA Restriction Analysis, Phenotype, Soil water, Species richness, business, Water Pollutants, Chemical |
| الوصف: | The genetic and functional diversity of Bacillus and Bacillus-derived genera was analyzed in soil samples collected from three different fields near Century Paper Mill, Lal Kuan, Uttarakhand, India. Two had been subjected to concentrated and diluted effluent irrigation for the past 25 years and were designated as a concentrated effluent irrigated field (CEIF) and a dilute effluent irrigated field (DEIF), respectively. The field irrigated with fresh water was designated as a water irrigated field (WIF). Increase in pH, Na and Zn content and decrease in Fe content was observed due to effluent irrigation. The population count of Bacillus and Bacillus-derived was maximum in DEIF followed by WIF and CEIF. Variations in plant growth-promoting traits and extracellular enzymes were observed among the isolates from the three different field soils. Based on the amplified ribosomal DNA restriction analysis (ARDRA) with three restriction enzymes, all the selected 104 isolates were clustered into 14 groups. The sequencing of the representative isolates revealed that the majority belonged to the genus Bacillus, while three isolates belonged to Paenibacillus, Lysinibacillus and Orthinibacillus. There were a few species like Orthinibacillus contaminans, B. oleronius, B. safensis, B. methylotrophicus, B. stratosphericus, B. aryabhattai, B. asahii and B. bataviensis that were prevalent only in DEIF and CEIF but not in WIF field soil. The diversity index parameters like the Shannon Index, indices of species richness and species evenness based on biochemical profiling and ARDRA profiling revealed that Bacillaceae members in the fresh water irrigated field were diverse. |
| تدمد: | 1349-8037 0022-1260 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::035ffcec99db20ada8a465f4dc789362Test https://doi.org/10.2323/jgam.57.183Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....035ffcec99db20ada8a465f4dc789362 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13498037 00221260 |
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