دورية أكاديمية
Heavy chain dominance in the binding of DNA by a lupus mouse monoclonal autoantibody.
| العنوان: | Heavy chain dominance in the binding of DNA by a lupus mouse monoclonal autoantibody. |
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| المؤلفون: | Jang, YJ, Lecerf, JM, Stollar, BD |
| المساهمون: | 101341, 장, 영주 |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Amino Acid Sequence, Animals, Antibodies, Antinuclear, Monoclonal, B-Lymphocytes, Base Sequence, Cloning, Molecular, DNA, DNA Primers, Escherichia coli, Immunoglobulin Fab Fragments, Immunoglobulin G, Immunoglobulin Heavy Chains, Immunoglobulin Light Chains, Immunoglobulin Variable Region, Lupus Erythematosus, Systemic, Mice, Molecular Sequence Data, Polymerase Chain Reaction |
| الوصف: | Antibodies H241 and 2C10 are lupus mouse IgG autoantibodies that bind native DNA. In previous experiments, oligonucleotide antigens affinity-labeled both H and L chains of H241 but only the H chain of antibody 2C10. Primary structures of the V regions of the 2C10 H and L chains and the H241 L chain, determined from cDNA, help to explain the previous affinity-labeling experiments. The 2C10 L chain CDRs had several Asp residues and a net negative charge of five, whereas the 2C10 H chain CDRs had four Arg residues and a net positive charge of five. The L chain CDRs of H241 had a net positive charge of one. [The H241 H chain cDNA sequence was published previously by Gangemi et al. (1993) J. Immun. 151, 4660-4671]. Plasmid vectors were used for bacterial expression of H and L chains of 2C10 alone and in combinations in single chain Fv (scFv) molecules. The H chain alone bound native DNA as well as or better than the H-plus-L chain scFv. The H chain alone also bound Z-DNA. Combination of the 2C10 H chain with the L chain of an anti-Z-DNA antibody maintained the selectivity for Z-DNA, whereas its combination with the 2C10 L chain (in the 2C10 Fab) yielded selective B-DNA binding. The results with 2C10 match other examples in which the H chain is sufficient for DNA binding but selectivity is modulated by the L chain. The H chain binding to autoantigen may reflect selective events in early stages of B cell development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0161-5890 1872-9142 |
| العلاقة: | J001615890; http://repository.ajou.ac.kr/handle/201003/4218Test; 8649441; http://linkinghub.elsevier.com/retrieve/pii/0161589095000941Test; PMID-8649441; Molecular immunology, 33(2). : 197-210, 1996 |
| الإتاحة: | http://repository.ajou.ac.kr/handle/201003/4218Test http://linkinghub.elsevier.com/retrieve/pii/0161589095000941Test |
| رقم الانضمام: | edsbas.88BE2FE1 |
| قاعدة البيانات: | BASE |
| تدمد: | 01615890 18729142 |
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