Single-cell intracellular epitope and transcript detection reveals signal transduction dynamics
| العنوان: | Single-cell intracellular epitope and transcript detection reveals signal transduction dynamics |
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| المؤلفون: | Hans van Eenennaam, Francesca Rivello, Wilhelm T. S. Huck, Paul Vink, Klaas W. Mulder, Kinga Matuła, Jessie A.G.L. van Buggenum, Erik van Buijtenen |
| المصدر: | Cell Reports Methods, 1, 5 Cell Reports Methods, 1 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cultural Studies, History, Literature and Literary Theory, Chemistry, B-cell receptor, RNA, Bio-Molecular Chemistry, Epitope, Cell biology, Transcriptome, Gene expression, Extracellular, Signal transduction, Molecular Developmental Biology, Intracellular, Physical Organic Chemistry |
| الوصف: | Summary To further our understanding of how biochemical information flows through cells upon external stimulation, we require single-cell multi-omics methods that concurrently map changes in (phospho)protein levels across signaling networks and the associated gene expression profiles. Here, we present quantification of RNA and intracellular epitopes by sequencing (QuRIE-seq), a droplet-based platform for single-cell RNA and intra- and extracellular (phospho)protein quantification through sequencing. We applied QuRIE-seq to quantify cell-state changes at both the signaling and the transcriptome level after 2-, 4-, 6-, 60-, and 180-min stimulation of the B cell receptor pathway in Burkitt lymphoma cells. Using the multi-omics factor analysis (MOFA+) framework, we delineated changes in single-cell (phospho)protein and gene expression patterns over multiple timescales and revealed the effect of an inhibitory drug (ibrutinib) on signaling and gene expression landscapes. |
| وصف الملف: | application/pdf |
| تدمد: | 2667-2375 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a596025104383956369e6680aa8e98aTest https://pubmed.ncbi.nlm.nih.gov/35474668Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8a596025104383956369e6680aa8e98a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26672375 |
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