التفاصيل البيبلوغرافية
| العنوان: |
Designing a rotatory device for development of 3D super-resolution tomographic STED microscopy |
| المؤلفون: |
Thalla, Divyendu Goud |
| المساهمون: |
Åbo Akademi, Fakulteten för naturvetenskaper och teknik, Biomedicinsk visualisering |
| سنة النشر: |
2021 |
| المجموعة: |
Doria (National Library of Finland / |
| مصطلحات موضوعية: |
STED, tomographic microscopy, vimentin, axial resolution, microscopy, optical microscopy, Three-dimensional imaging, mikroskopi, ljusmikroskopi, 3D-modellering, 119 Övriga naturvetenkaper |
| الوصف: |
In this master thesis, multiple angle 3D tomographic STED microscopy technique was developed to implement 3D super resolution imaging to thicker samples. Firstly, a rotating stage was designed and manufactured to be incorporated in the STED microscope setup. This rotator can hold biological samples ranging from single cell to cell clusters in a micro glass capillary of 50 μm inner diameter. Glass capillary was attached to the rotator such that it gives the user flexibility to rotate the sample in 360 degrees and acquire images of the sample using the super resolution STED microscope. Vimentin cytoskeletal protein in BHK-21 and K562 cells was imaged using this technique. Primarily using BHK-21 cells we showed the precision of our rotating setup which achieved STED imaging of same single cell in multiple angles. By using K562 cell cluster we demonstrated that our technique could take 3D tomographic images of thick samples which was previously restricted due to working distance limitation of the objective. This technique opens up the possibilities of applying 3D super resolution STED. microscopy to thick tissues for example tumours can be imaged. |
| نوع الوثيقة: |
thesis |
| اللغة: |
English |
| العلاقة: |
https://www.doria.fi/handle/10024/181436Test; URN:NBN:fi-fe2021061537238 |
| الإتاحة: |
https://www.doria.fi/handle/10024/181436Test |
| حقوق: |
fi=All rights reserved. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.|sv=All rights reserved. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.|en=All rights reserved. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.| |
| رقم الانضمام: |
edsbas.60F9715B |
| قاعدة البيانات: |
BASE |
