المؤلفون: Helge Tolleshaug, Paul B. Gordon, Henrik Hoyvik, Per Ottar Seglen

المصدر: Biochemical Society Transactions. 13:1007-1010

مصطلحات موضوعية: Sucrose, Chemistry, Adenine, Autophagy, Proteins, Mitochondria, Liver, Protein degradation, Cell Fractionation, Biochemistry, Cell Compartmentation, Rats, Cell biology, Liver, Phagocytosis, Animals, Autolysis, Lysosomes

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::392aa28c27175787e5455cf8cba2dcccTest
https://doi.org/10.1042/bst0131007Test

المؤلفون: P. B. Gordon, Per Ottar Seglen

مصطلحات موضوعية: Purine, Liver cytology, Protein degradation, Biology, chemistry.chemical_compound, Adenosine Triphosphate, Phagocytosis, Protein biosynthesis, Animals, Amino Acids, Biological Sciences: Cell Biology, Cells, Cultured, chemistry.chemical_classification, Multidisciplinary, Adenine, Autophagy, Proteins, Cell biology, Amino acid, Rats, Biochemistry, chemistry, Liver, Protein Biosynthesis, Autolysis, Lysosomes, Adenosine triphosphate, Intracellular

الوصف: 3-Methyladenine (5 mM) inhibits endogenous protein degradation in isolated rat hepatocytes by about 60%, while having no adverse effect on the degradation of an exogenous protein (asialofetuin), on protein synthesis, or on intracellular ATP levels. 3-Methyladenine appears to act specifically upon the autophagic/lysosomal pathway of degradation, as judged from its lack of effect in the presence of amino acids or a lysosomotropic amine (propylamine). The effect of the purine is not mediated by amino acids because the inhibition of protein degradation is accompanied by a significant depression of intracellular amino acid levels. The ability of 3-methyladenine to suppress the formation of electron microscopically visible autophagosomes suggests that it may be regarded as a specific inhibitor of autophagy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1babbf577c2810d389d0036c16cd81Test
https://europepmc.org/articles/PMC346086Test/