التفاصيل البيبلوغرافية
| العنوان: |
IRF5 gene polymorphisms in melanoma. |
| المؤلفون: |
Uccellini, Lorenzo, De Giorgi, Valeria, Yingdong Zhao, Tumaini, Barbara, Erdenebileg, Narnygerel, Dudley, Mark E., Tomei, Sara, Bedognetti, Davide, Libera Ascierto, Maria, Qiuzhen Liu, Simon, Richard, Kottyan, Leah, Kaufman, Kenneth M., Harley, John B., Ena Wang, Rosenberg, Steven A., Marincola, Francesco M. |
| المصدر: |
Journal of Translational Medicine; 2012, Vol. 10 Issue 1, p170-177, 8p, 3 Color Photographs, 3 Charts |
| مصطلحات موضوعية: |
INTERFERON regulatory factors, TRANSCRIPTION factors, MELANOMA, AUTOIMMUNITY, SINGLE nucleotide polymorphisms, LYMPHOCYTES |
| مستخلص: |
Background: Interferon regulatory factor (IRF)-5 is a transcription factor involved in type I interferon signaling whose germ line variants have been associated with autoimmune pathogenesis. Since relationships have been observed between development of autoimmunity and responsiveness of melanoma to several types of immunotherapy, we tested whether polymorphisms of IRF5 are associated with responsiveness of melanoma to adoptive therapy with tumor infiltrating lymphocytes (TILs). Methods: 140 TILs were genotyped for four single nucleotide polymorphisms (rs10954213, rs11770589, rs6953165, rs2004640) and one insertion-deletion in the IRF5 gene by sequencing. Gene-expression profile of the TILs, 112 parental melanoma metastases (MM) and 9 cell lines derived from some metastases were assessed by Affymetrix Human Gene ST 1.0 array. Results: Lack of A allele in rs10954213 (G > A) was associated with non-response (p < 0.005). Other polymorphisms in strong linkage disequilibrium with rs10954213 demonstrated similar trends. Genes differentially expressed in vitro between cell lines carrying or not the A allele could be applied to the transcriptional profile of 112 melanoma metastases to predict their responsiveness to therapy, suggesting that IRF5 genotype may influence immune responsiveness by affecting the intrinsic biology of melanoma. Conclusions: This study is the first to analyze associations between melanoma immune responsiveness and IRF5 polymorphism. The results support a common genetic basis which may underline the development of autoimmunity and melanoma immune responsiveness. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Translational Medicine is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder