Molecular and Biochemical Techniques for Deciphering p53-MDM2 Regulatory Mechanisms
| العنوان: | Molecular and Biochemical Techniques for Deciphering p53-MDM2 Regulatory Mechanisms |
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| المؤلفون: | Ana M Peña-Balderas, Ignacio López, Vanesa Olivares-Illana, Robin Fåhareus, Konstantinos Karakostis |
| المساهمون: | Karakostis K., López Ferreira Luis Ignacio, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Peña-Balderas A. M., Fåhareus R., Olivares-Illana V. |
| المصدر: | Biomolecules COLIBRI Universidad de la República instacron:Universidad de la República Biomolecules, Vol 11, Iss 36, p 36 (2021) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, p53, MDMX, Multiple Partners, lcsh:QR1-502, protein-protein interactions, p53 mRNA, Cell Cycle Proteins, Computational biology, Review, DNA damage response, Biochemistry, P53 mdm2, lcsh:Microbiology, Protein–protein interaction, 03 medical and health sciences, Mice, 0302 clinical medicine, MDM2, Animals, Humans, Phosphorylation, Molecular Biology, protein-RNA interactions, biology, Chemistry, Protein dynamics, Biochemistry and Molecular Biology, Nuclear Proteins, Proto-Oncogene Proteins c-mdm2, 030104 developmental biology, post-translational modification, 030220 oncology & carcinogenesis, ATM, biology.protein, Posttranslational modification, Mdm2, Regulatory Pathway, Tumor Suppressor Protein p53, Protein Processing, Post-Translational, Biokemi och molekylärbiologi, DNA Damage, Protein Binding |
| الوصف: | The p53 and Mouse double minute 2 (MDM2) proteins are hubs in extensive networks of interactions with multiple partners and functions. Intrinsically disordered regions help to adopt function-specific structural conformations in response to ligand binding and post-translational modifications. Different techniques have been used to dissect interactions of the p53-MDM2 pathway, in vitro, in vivo, and in situ each having its own advantages and disadvantages. This review uses the p53-MDM2 to show how different techniques can be employed, illustrating how a combination of in vitro and in vivo techniques is highly recommended to study the spatio-temporal location and dynamics of interactions, and to address their regulation mechanisms and functions. By using well-established techniques in combination with more recent advances, it is possible to rapidly decipher complex mechanisms, such as the p53 regulatory pathway, and to demonstrate how protein and nucleotide ligands in combination with post-translational modifications, result in inter-allosteric and intra-allosteric interactions that govern the activity of the protein complexes and their specific roles in oncogenesis. This promotes elegant therapeutic strategies that exploit protein dynamics to target specific interactions. |
| وصف الملف: | application/pdf |
| تدمد: | 2218-273X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8e23b210e4fcc1a369f07413b95d3c7Test https://pubmed.ncbi.nlm.nih.gov/33396576Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f8e23b210e4fcc1a369f07413b95d3c7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2218273X |
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