Altered transcriptional and chromatin responses to rhinovirus in bronchial epithelial cells from adults with asthma
| العنوان: | Altered transcriptional and chromatin responses to rhinovirus in bronchial epithelial cells from adults with asthma |
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| المؤلفون: | James E. Gern, Christine Billstrand, Carole Ober, Marcelo A. Nobrega, Britney A Helling, Kaixuan Luo, Steven R. White, Grace T Hansen, Raluca Nicolae, Débora R. Sobreira, Dan L. Nicolae, Noboru J. Sakabe, Bharathi Laxman, Yury A. Bochkov |
| المصدر: | Communications Biology, Vol 3, Iss 1, Pp 1-11 (2020) Communications Biology |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Epigenomics, 0301 basic medicine, Rhinovirus, Transcription, Genetic, Medicine (miscellaneous), Genome-wide association study, Respiratory Mucosa, Biology, medicine.disease_cause, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, immune system diseases, Gene expression, medicine, Humans, Genetic risk, lcsh:QH301-705.5, Gene, Cells, Cultured, Asthma, Epithelial Cells, Antimicrobial responses, respiratory system, medicine.disease, Chromatin, Gene regulation, respiratory tract diseases, 030104 developmental biology, lcsh:Biology (General), Apoptosis, 030220 oncology & carcinogenesis, Immunology, General Agricultural and Biological Sciences |
| الوصف: | There is a life-long relationship between rhinovirus (RV) infection and the development and clinical manifestations of asthma. In this study we demonstrate that cultured primary bronchial epithelial cells from adults with asthma (n = 9) show different transcriptional and chromatin responses to RV infection compared to those without asthma (n = 9). Both the number and magnitude of transcriptional and chromatin responses to RV were muted in cells from asthma cases compared to controls. Pathway analysis of the transcriptionally responsive genes revealed enrichments of apoptotic pathways in controls but inflammatory pathways in asthma cases. Using promoter capture Hi-C we tethered regions of RV-responsive chromatin to RV-responsive genes and showed enrichment of these regions and genes at asthma GWAS loci. Taken together, our studies indicate a delayed or prolonged inflammatory state in cells from asthma cases and highlight genes that may contribute to genetic risk for asthma. Britney Helling et al. report that cultured bronchial cells from adults with asthma show different gene expression and chromatin accessibility patterns when exposed to rhinovirus than do cells from individuals without asthma. Their data suggest that rhinovirus infection leads to a delayed or elongated activation of inflammatory genes in individuals with asthma compared to those without asthma. |
| تدمد: | 2399-3642 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee35b336cab85a82108dfe6f4f1e811cTest https://doi.org/10.1038/s42003-020-01411-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ee35b336cab85a82108dfe6f4f1e811c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23993642 |
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