Molecular Tools for the Detection and Deduction of Azole Antifungal Drug Resistance Phenotypes in Aspergillus Species

التفاصيل البيبلوغرافية
العنوان: Molecular Tools for the Detection and Deduction of Azole Antifungal Drug Resistance Phenotypes in Aspergillus Species
المؤلفون: Birgit Spiess, Uwe Groß, Michael Weig, Anna Dudakova, Dieter Buchheidt, Christoph Sasse, Marut Tangwattanachuleeporn, Oliver Bader
المصدر: Clinical microbiology reviews. 30(4)
سنة النشر: 2017
مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Drug, Azoles, Antifungal Agents, Epidemiology, media_common.quotation_subject, 030106 microbiology, Aspergillus flavus, Drug resistance, Microbial Sensitivity Tests, Review, Aspergillosis, Microbiology, Aspergillus fumigatus, Fungal Proteins, 03 medical and health sciences, Sterol 14-Demethylase, Drug Resistance, Fungal, medicine, Humans, Aspergillus terreus, media_common, chemistry.chemical_classification, General Immunology and Microbiology, biology, Aspergillus niger, Public Health, Environmental and Occupational Health, biology.organism_classification, medicine.disease, 3. Good health, Infectious Diseases, Aspergillus, Phenotype, chemistry, Azole
الوصف: SUMMARY The incidence of azole resistance in Aspergillus species has increased over the past years, most importantly for Aspergillus fumigatus . This is partially attributable to the global spread of only a few resistance alleles through the environment. Secondary resistance is a significant clinical concern, as invasive aspergillosis with drug-susceptible strains is already difficult to treat, and exclusion of azole-based antifungals from prophylaxis or first-line treatment of invasive aspergillosis in high-risk patients would dramatically limit drug choices, thus increasing mortality rates for immunocompromised patients. Management options for invasive aspergillosis caused by azole-resistant A. fumigatus strains were recently reevaluated by an international expert panel, which concluded that drug resistance testing of cultured isolates is highly indicated when antifungal therapy is intended. In geographical regions with a high environmental prevalence of azole-resistant strains, initial therapy should be guided by such analyses. More environmental and clinical screening studies are therefore needed to generate the local epidemiologic data if such measures are to be implemented on a sound basis. Here we propose a first workflow for evaluating isolates from screening studies, and we compile the MIC values correlating with individual amino acid substitutions in the products of cyp51 genes for interpretation of DNA sequencing data, especially in the absence of cultured isolates.
تدمد: 1098-6618
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a098d29783cc93c006b3d8e342dd2ce2Test
https://pubmed.ncbi.nlm.nih.gov/28903985Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....a098d29783cc93c006b3d8e342dd2ce2
قاعدة البيانات: OpenAIRE