المؤلفون: Graziella Bonetti, Sara Saggini, Annamaria Menolfi, Antonio Marino, Filippo Manelli, Giuseppe Lippi, Alessandra Bettinardi, Andrea Patroni, Gianluca Borrelli, Gianfranco Fiordalisi, R. Volpi, A. Anesi

مصطلحات موضوعية: Blood Glucose, Male, Neutrophils, Clinical Biochemistry, coronavirus, Comorbidity, 030204 cardiovascular system & hematology, Gastroenterology, Laboratory, chemistry.chemical_compound, 0302 clinical medicine, COVID-19 Testing, Leukocytes, Urea, 030212 general & internal medicine, Hospital Mortality, Lymphocytes, Creatine Kinase, Aged, 80 and over, medicine.diagnostic_test, biology, Carnosine, Age Factors, General Medicine, Middle Aged, Drug Combinations, C-Reactive Protein, Italy, Creatinine, Population study, Female, Partial Thromboplastin Time, Coronavirus Infections, Partial thromboplastin time, medicine.medical_specialty, Pneumonia, Viral, Serum Albumin, Human, Arginine, Fibrin Fibrinogen Degradation Products, 03 medical and health sciences, Betacoronavirus, Internal medicine, Lactate dehydrogenase, death, medicine, Humans, Aspartate Aminotransferases, Pandemics, Aged, Prothrombin time, L-Lactate Dehydrogenase, business.industry, Clinical Laboratory Techniques, SARS-CoV-2, Biochemistry (medical), C-reactive protein, Troponin I, COVID-19, medicine.disease, chemistry, Ferritins, biology.protein, Prothrombin Time, Creatine kinase, death, coronavirus, COVID-19, business

الوصف: Background Comprehensive information has been published on laboratory tests which may predict worse outcome in Asian populations with coronavirus disease 2019 (COVID-19). The aim of this study is to describe laboratory findings in a group of Italian COVID-19 patients in the area of Valcamonica, and correlate abnormalities with disease severity. Methods The final study population consisted of 144 patients diagnosed with COVID-19 (70 who died during hospital stay and 74 who survived and could be discharged) between March 1 and 30, 2020, in Valcamonica Hospital. Demographical, clinical and laboratory data were collected upon hospital admission and were then correlated with outcome (i.e. in-hospital death vs. discharge). Results Compared to patients who could be finally discharged, those who died during hospital stay displayed significantly higher values of serum glucose, aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), urea, creatinine, high-sensitivity cardiac troponin I (hscTnI), prothrombin time/international normalized ratio (PT/INR), activated partial thromboplastin time (APTT), D-dimer, C reactive protein (CRP), ferritin and leukocytes (especially neutrophils), whilst values of albumin, hemoglobin and lymphocytes were significantly decreased. In multiple regression analysis, LDH, CRP, neutrophils, lymphocytes, albumin, APTT and age remained significant predictors of in-hospital death. A regression model incorporating these variables explained 80% of overall variance of in-hospital death. Conclusions The most important laboratory abnormalities described here in a subset of European COVID-19 patients residing in Valcamonica are highly predictive of in-hospital death and may be useful for guiding risk assessment and clinical decision-making.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42a4d4351db4885eac4ec49ca1173437Test
http://hdl.handle.net/11562/1019405Test

المؤلفون: Pan Ji, Bocheng Li, Xiaowen Zheng, Jieyun Zhu, Hongyuan Li, Mingchun Ou, Jianfeng Zhang, Jielong Pang, Zhimei Zhong

المصدر: Epidemiology and Infection

مصطلحات موضوعية: 0301 basic medicine, Epidemiology, critically ill, Lymphocyte, Severity of Illness Index, Gastroenterology, Procalcitonin, Leukocyte Count, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, 030212 general & internal medicine, severe disease, Coronavirus disease 2019, biology, Age Factors, Alanine Transaminase, C-Reactive Protein, Infectious Diseases, medicine.anatomical_structure, Creatinine, Coronavirus Infections, medicine.medical_specialty, Critical Illness, Pneumonia, Viral, Fibrin Fibrinogen Degradation Products, Betacoronavirus, 03 medical and health sciences, Lymphopenia, Internal medicine, White blood cell, Lactate dehydrogenase, Severity of illness, medicine, Humans, Aspartate Aminotransferases, Lymphocyte Count, Pandemics, Original Paper, L-Lactate Dehydrogenase, Platelet Count, SARS-CoV-2, business.industry, C-reactive protein, COVID-19, Thrombocytopenia, meta-analysis, 030104 developmental biology, Alanine transaminase, chemistry, biology.protein, business

الوصف: Our study aimed to systematically analyse the risk factors of coronavirus disease 2019 (COVID-19) patients with severe disease. An electronic search in eight databases to identify studies describing severe or critically ill COVID-19 patients from 1 January 2020 to 3 April 2020. In the end, we meta-analysed 40 studies involving 5872 COVID-19 patients. The average age was higher in severe COVID-19 patients (weighted mean difference; WMD = 10.69, 95%CI 7.83–13.54). Patients with severe disease showed significantly lower platelet count (WMD = −18.63, 95%CI −30.86 to −6.40) and lymphocyte count (WMD = −0.35, 95%CI −0.41 to −0.30) but higher C-reactive protein (CRP; WMD = 42.7, 95%CI 31.12–54.28), lactate dehydrogenase (LDH; WMD = 137.4, 95%CI 105.5–169.3), white blood cell count（WBC), procalcitonin（PCT）, D-dimer, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatinine（Cr）. Similarly, patients who died showed significantly higher WBC, D-dimer, ALT, AST and Cr but similar platelet count and LDH as patients who survived. These results indicate that older age, low platelet count, lymphopenia, elevated levels of LDH, ALT, AST, PCT, Cr and D-dimer are associated with severity of COVID-19 and thus could be used as early identification or even prediction of disease progression.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4486a18cef4abf73093a39286321e1eTest
https://doi.org/10.1017/s095026882000179xTest

المؤلفون: P. Caropreso, Nicola Lavorato, Giorgia Catalano, Giorgia Rizza, Giulio Mengozzi, Renato Romagnoli, Damiano Patrono, Paola Berchialla, Alessandro Gambella

مصطلحات موضوعية: Male, medicine.medical_specialty, 030230 surgery, Liver weight, Gastroenterology, Alanine Transaminase, Aspartate Aminotransferases, Cold Temperature, Female, Graft Survival, Humans, Hydrogen-Ion Concentration, L-Lactate Dehydrogenase, Liver Transplantation, Middle Aged, Organ Preservation, Perfusion, Retrospective Studies, Tissue Donors, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, Internal medicine, medicine, Transplantation, Machine perfusion, business.industry, Acute kidney injury, Odds ratio, medicine.disease, Confidence interval, chemistry, 030211 gastroenterology & hepatology, Graft survival, Complication, business

الوصف: BACKGROUND Liver graft viability assessment has long been considered a limit of hypothermic oxygenated machine perfusion (HOPE). Aim of this study was assessing correlations of easily available perfusate parameters (PP) (aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, glucose, lactate, and pH) with graft features and outcome. METHODS In the period October 2018-February 2020, perfusate samples were obtained every 30 minutes during 50 dual-HOPE (D-HOPE) procedures. Correlations of PP with graft factors, 90-day graft loss, early allograft dysfunction (EAD), L-GrAFT score, acute kidney injury, and comprehensive complication index were analyzed using Pearson coefficient, receiver-operating characteristics analysis and by univariable and multivariable regression. RESULTS Median D-HOPE time was 122 minutes. All parameters were normalized to liver weight. Only macrovesicular steatosis (MaS) significantly impacted PP levels and slope. Grafts with ≥30% MaS exhibited significantly different PP values and slope. Graft loss and EAD rate were 2% (n = 1) and 26% (n = 13). All PP except lactate correlated with EAD, 90-minute alanine aminotransferase showing the highest area under the receiver-operating characteristics curve (0.84). However, at multivariable analysis, the only factor independently associated with EAD was MaS (odds ratio, 5.44; confidence interval, 1.05-28.21; P = 0.04). Ninety minutes lactate dehydrogenase had the strongest correlation with L-GrAFT (R = 0.70; P < 0.001). PP correlated poorly with comprehensive complication index and grades 2-3 acute kidney injury rate. CONCLUSIONS PP were predictive of graft function after transplant, but their association with graft survival and clinical outcomes requires further evaluation. MaS influenced levels of PP and was the only independent predictor of EAD.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::990451e5bd8494b6a2c5fc8503a9ffb3Test
http://hdl.handle.net/2318/1770090Test

المؤلفون: Soledad Arribalzaga, Aitor Viribay, Juan Mielgo-Ayuso, Arkaitz Castañeda-Babarro, Jesús Seco-Calvo, Aritz Urdampilleta

المصدر: UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname
Nutrients
Volume 12
Issue 5
Nutrients, Vol 12, Iss 1367, p 1367 (2020)

مصطلحات موضوعية: Male, Marathon Running, Perceived exertion, Muscle damage, chemistry.chemical_compound, 0302 clinical medicine, Urea, Ingestion, Medicine, Creatine Kinase, Nutrition and Dietetics, biology, Glycogen, Muscle recovery, Middle Aged, Creatinine, glycogen, lcsh:Nutrition. Foods and food supply, Adult, education, lcsh:TX341-641, 030209 endocrinology & metabolism, Article, Glucógeno, 03 medical and health sciences, Músculos - Recuperación, Athletic performance, Animal science, muscle recovery, Muscular Diseases, Lactate dehydrogenase, Dietary Carbohydrates, Humans, Aspartate Aminotransferases, Rendimiento deportivo, Muscle, Skeletal, Carbohydrates intake, Exercise, L-Lactate Dehydrogenase, business.industry, Dietary intake, 030229 sport sciences, chemistry, Physical Endurance, biology.protein, Creatine kinase, athletic performance, business, dietary intake, human activities, Ingesta, Food Science

الوصف: Background&mdash
exercise-induced muscle damage (EIMD) and internal exercise load are increased after competing in ultraendurance events such as mountain marathons. Adequate carbohydrate (CHO) intake during exercise optimizes athletic performance and could limit EIMD, reduce internal exercise load and, thus, improve recovery. Therefore, the aim of this study was to research into and compare the effects of high CHO intake (120 g/h) in terms of CHO intake recommendation (90 g/h) and regular CHO intake performed by ultraendurance athletes (60 g/h) during a mountain marathon, on exercise load and EIMD markers (creatine kinase (CK), lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT), urea and creatinine). Materials and Methods&mdash
a randomized trial was carried out on 20 male elite runners who had previously undertaken nutritional and gut training, and who consumed different CHO dosages according to experimental (EXP&mdash
120 g/h), control (CON&mdash
90 g/h) and low CHO intake (LOW&mdash
60 g/h) groups during a ~4000 m cumulative slope mountain marathon. EIMD markers were analyzed before the race and 24 h afterwards. Internal exercise load was calculated based on rate of perceived exertion (RPE) during and after the marathon event. Results&mdash
internal exercise load during the mountain marathon was significantly lower (p = 0.019
&eta
2p = 0.471) in EXP (3805 ±
281 AU) compared to LOW (4688 ±
705 AU) and CON (4692 ±
716 AU). Moreover, results revealed that the EXP group evidenced significantly lower CK (p = 0.019
2p = 0.373), LDH (p <
0.001
2p = 0.615) and GOT (p = 0.003
2p = 0.500) values 24 h after the mountain marathon race compared to LOW and CON. Along these lines, EIMD and exercise load evidenced a close correlation (R = 0.742
p <
0.001). Conclusion: High CHO intake (120 g/h) during a mountain marathon could limit the EIMD observed by CK, LDH and GOT and internal exercise load compared to CHO ingestion of 60 and 90 g/h.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ace778e572376b1468c953b10b0b0117Test
https://uvadoc.uva.es/handle/10324/52265Test

المؤلفون: Kamil Pehlivanoglu, Ferda Keskin Cimen, Halis Suleyman, Nezahat Kurt, Bahadir Suleyman, Eray Kurnaz, Aslı Özbek Bilgin, Renad Mammadov, Huseyin Eken, Arif Burak Cekic, Orhan Cimen

المصدر: BMC Pharmacology & Toxicology
BMC Pharmacology and Toxicology, Vol 21, Iss 1, Pp 1-9 (2020)

مصطلحات موضوعية: Male, Antioxidant, medicine.medical_treatment, Injury, Pharmacology, Antioxidants, chemistry.chemical_compound, 0302 clinical medicine, Malondialdehyde, Pharmacology (medical), Group level, Glutathione Transferase, 0303 health sciences, biology, virus diseases, Alanine Transaminase, Liver ischemia, Glutathione, Drug Combinations, Glutathione Reductase, Liver, 030220 oncology & carcinogenesis, Myeloperoxidase, Reperfusion Injury, Research Article, Liv-52, Ischemia, Superoxide dismutase, 03 medical and health sciences, lcsh:RA1190-1270, Lactate dehydrogenase, medicine, Animals, Aspartate Aminotransferases, Rats, Wistar, 030304 developmental biology, lcsh:Toxicology. Poisons, Peroxidase, Glutathione Peroxidase, L-Lactate Dehydrogenase, business.industry, Plant Extracts, Superoxide Dismutase, lcsh:RM1-950, medicine.disease, Ischemia reperfusion, lcsh:Therapeutics. Pharmacology, chemistry, biology.protein, Rat, business

الوصف: Background Liver ischemia reperfusion (I/R) damage which is frequently seen in clinical hepatobiliary surgeries has no effective treatment for it. Liv-52, known to have hepatoprotective effects, is a natural antioxidant drug licensed by the Ministry of Health of India. The aim of our study is to investigate the effect of Liv-52 on liver damage induced by I/R in rats. Methods Albino Wistar male rats were divided into three groups; liver I/R (IR), 20 mg/kg Liv-52 + liver ischemia reperfusion (LIR) and sham operation applied to control group (HG). Liv-52 was administered to the LIR group (n = 6) 1 h prior to I/R application and distilled water was given orally to IR (n = 6) and HG (n = 6) groups as a solvent. Ischemia was determined as 1 h, and reperfusion was identified as 6 h in animals. Results Increased levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, malondialdehyde, myeloperoxidase, and decreased levels of superoxide dismutase, and glutathione related enzymes caused by I/R application have been converged to healthy group level with Liv-52 treatment and the damage in liver tissue has been improved histopathologically. Conclusions Liv-52 may be beneficial for preventing liver I/R damage in pre-surgery application.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03b511f1c6215aa67e8c9e8973439835Test
https://doi.org/10.21203/rs.2.14473/v3Test

المؤلفون: Yi Long, Yahui Zhang, Jihua Chen, Ruixue Huang, Guofeng Ren, Lina Yang, Jingfang Luo

المصدر: Journal of Medicinal Food

مصطلحات موضوعية: 0301 basic medicine, Male, Serum, Antioxidant, medicine.medical_treatment, Medicine (miscellaneous), Pharmacology, medicine.disease_cause, Antioxidants, Pomegranate, chemistry.chemical_compound, Mice, 0302 clinical medicine, Malondialdehyde, Carbon Tetrachloride, chemistry.chemical_classification, Liver injury, Mice, Inbred ICR, Nutrition and Dietetics, biology, Glutathione peroxidase, Forkhead Box Protein O3, Alanine Transaminase, Hydrolyzable Tannins, Oncogene Protein v-akt, Liver, antioxidation, 030220 oncology & carcinogenesis, Chemical and Drug Induced Liver Injury, Full Communications, Signal Transduction, liver injury, autophagy, Cell Survival, NF-E2-Related Factor 2, Cell Line, Superoxide dismutase, 03 medical and health sciences, Lactate dehydrogenase, medicine, Animals, Humans, Aspartate Aminotransferases, polyphenols, Punicalagin, Glutathione Peroxidase, 030109 nutrition & dietetics, L-Lactate Dehydrogenase, Plant Extracts, Superoxide Dismutase, medicine.disease, Oxidative Stress, chemistry, punicalagin, biology.protein, Oxidative stress

الوصف: Hepatic injury is significant in the pathogenesis and development of many types of liver diseases. Punicalagin (PU) is a bioactive antioxidant polyphenol found in pomegranates. To explore its protective effect against carbon tetrachloride (CCl4)-induced liver injury and the mechanism, Institute of Cancer Research (ICR) mice and L02 cells were used to observe the changes of serum biochemical indicators, histopathological liver structure, cell viability, antioxidative indices, and autophagy-related proteins were assessed. In ICR mice, PU ameliorated the CCl4-induced increase of the serum aspartate aminotransferase, alanine aminotransferase, the activity of liver lactate dehydrogenase, and the damage of histopathological structure, and exhibited a hepatoprotective effect against CCl4. PU attenuated oxidative stress by decreasing the liver malondialdehyde level and increasing the activities of liver superoxide dismutase, glutathione peroxidase, and the expression of the liver nuclear factor E2-related factor (Nrf2) protein. Furthermore, according to the vivo and vitro experiments, PU might activate autophagy through the mediation of the Akt/FOXO3a and P62/Nrf2 signaling pathway. Taken together, these results suggest that PU may protect against CCl4-induced liver injury through the upregulation of antioxidative activities and autophagy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb113bd6cda1e2a6e33233f7281f8689Test
https://pubmed.ncbi.nlm.nih.gov/31718395Test

المؤلفون: Peng Shu, Bin Zhou, Jizhou Zhang, Jingling Song, Qing Li, Qiqi Shao

المصدر: Journal of Cellular Biochemistry. 119:8410-8418

مصطلحات موضوعية: 0301 basic medicine, Anti-Inflammatory Agents, Panax notoginseng, Aspartate transaminase, Pharmacology, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Hydroxyproline, 0302 clinical medicine, Western blot, Lactate dehydrogenase, Pulmonary fibrosis, medicine, Animals, Aspartate Aminotransferases, Interleukin 8, Creatine Kinase, Molecular Biology, Analysis of Variance, L-Lactate Dehydrogenase, biology, medicine.diagnostic_test, Interleukin-6, business.industry, Interleukin-8, NF-kappa B, Cell Biology, Saponins, medicine.disease, biology.organism_classification, Idiopathic Pulmonary Fibrosis, Disease Models, Animal, Treatment Outcome, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, biology.protein, Prednisone, Creatine kinase, Rabbits, business, Phytotherapy, Signal Transduction

الوصف: Panax notoginseng saponin (PNS) constitutes the major effective components of Panax notoginseng, which is widely used to treat microcirculatory disturbance associated diseases. In this study, we designed to investigate the effect of PNS on the treatment of pulmonary fibrosis (PF) and further explored its mechanism. A total of 40 healthy Japanese White rabbits were randomly divided into five groups (control group; PF model group; PNS prevention group; PNS treatment group; and western medicine [prednisone acetate] treatment group). Expression of hydroxyproline (HYP), fibronectin (FN), aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), interleukin-6 (IL-6), and interleukin-8 (IL-8) in serum was detected using corresponding detection kits. Western blot was applied to detect the expression of p50 and p65 in pulmonary tissues. The pathological variations of the cardiac and pulmonary ultrastructure were observed under both the optical and electron microscope. PF models were established successfully. The results showed that compared with the other groups, PNS groups (PNS prevention and treatment group) apparently relieved the cardiopulmonary injury, and reduced IL-6 and IL-8 expression levels in the serum. Furthermore, the PNS groups performed better in relieving cardiopulmonary injurythan other groups. Both the PNS groups and the western medicine treatment group presented an obvious role in relieving PF. We concluded that PNS could reduce the expression of AST, LDH, CK, IL-6, and IL-8 in serum of the rabbits, relieve the pathological ultrastructure of cardiopulmonary injury, alleviate PF. And it might be attributed to the inhibition on the NF-κB signaling pathway.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c98e14f5923f658e27998317a1bc6cb0Test
https://doi.org/10.1002/jcb.27045Test

المؤلفون: Mansour I. Almansour, Bashir M. Jarrar, Saud Alarifi

المصدر: International Journal of Nanomedicine

مصطلحات موضوعية: Male, 0301 basic medicine, biochemical alterations, Pharmaceutical Science, 02 engineering and technology, Hydropic degeneration, chemistry.chemical_compound, International Journal of Nanomedicine, Drug Discovery, morphometric alterations, Original Research, Whole blood, Hematology, Chemistry, Alanine Transaminase, General Medicine, Hyperplasia, Silicon Dioxide, 021001 nanoscience & nanotechnology, histological alterations, Liver, Inactivation, Metabolic, Toxicity, Alkaline phosphatase, Chemical and Drug Induced Liver Injury, 0210 nano-technology, Injections, Intraperitoneal, medicine.medical_specialty, Kupffer Cells, Biophysics, Bioengineering, Biomaterials, 03 medical and health sciences, Internal medicine, Lactate dehydrogenase, medicine, Animals, Aspartate Aminotransferases, Rats, Wistar, L-Lactate Dehydrogenase, hematological alterations, Organic Chemistry, toxicity, Alkaline Phosphatase, medicine.disease, 030104 developmental biology, Endocrinology, Gene Expression Regulation, gene expression, Nanoparticles, Histopathology

الوصف: Mansour Almansour,1 Saud Alarifi,1 Bashir Jarrar21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biological Sciences, College of Science, Jerash University, Jerash, JordanBackground: Silicon dioxide (silica) nanoparticles (SDNPs) are widely used in nanotechnology and medicine, but these nanomaterials may carry a high risk for human health while little is known about their toxicity. Methods: We investigated the alterations in morphometry, biochemistry, hematology, histology of liver tissue and gene expression of drug-metabolizing enzymes induced by 10-nm SDNPs. Healthy male Wistar albino rats were exposed to 20, 35 and 50 repeated injections of SDNPs (2mg/kg body weight). Whole blood, serum and plasma samples were used for hematological and biochemical analyses, whereas liver biopsies were processed for histopathological and gene expression alterations. Results: In comparison with control rats, exposure to SDNPs lowered the body weight gain and liver index and increased the counts of white blood cells and platelets, but lowered the platelet larger cell ratio and plateletcrit. Levels of alkaline phosphatase, lactate dehydrogenase, low-density lipids, procalcitonin, aspartate aminotransferase and alanine aminotransferase, as well as potassium, phosphorus and iron concentrations, were increased. Histopathology revealed that SDNPs could induce hydropic degeneration, sinusoidal dilatation, hyperplasia of Kupffer cells, karyopyknosis and infiltration of inflammatory cells in the liver. SDNPs reduced the expression of 12 genes of drug-metabolizing enzymes significantly (p

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0497d0c43f4e274e327ea008290df4b0Test
https://doi.org/10.2147/ijn.s162847Test

المؤلفون: Mahmoud S. Arbid, Khaled M. M. Koriem

المصدر: Journal of Dietary Supplements. 16:152-165

مصطلحات موضوعية: Male, 0301 basic medicine, medicine.medical_specialty, Bilirubin, Antioxidants, Rats, Sprague-Dawley, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Phenols, Internal medicine, Lactate dehydrogenase, medicine, Animals, Pharmacology (medical), Aspartate Aminotransferases, chemistry.chemical_classification, 030109 nutrition & dietetics, Nutrition and Dietetics, L-Lactate Dehydrogenase, biology, Glutathione peroxidase, Acid phosphatase, Alanine Transaminase, DNA, gamma-Glutamyltransferase, 030229 sport sciences, Malondialdehyde, Rats, Oxidative Stress, Endocrinology, Liver, chemistry, Catalase, biology.protein, Chemical and Drug Induced Liver Injury, Corn oil, Food Science

الوصف: The endocrine-disrupting chemical 4-tert-octylphenol (OP) can mimic estrogen and testosterone hormones and threaten health; fertaric acid (FA) is a hydroxycinnamic acid found in grapefruit. This study aimed to investigate whether FA has a protective effect on 4-tert-octylphenol-related hepatotoxicity. Thirty male albino rats were divided into 5 equal groups of 6 rats each as follows: control group-administrated orally with 1 ml saline 3 days/week for 4 weeks; corn oil group-administrated orally with 1 ml corn oil 3 days/week for 4 weeks; FA-treated group-administrated orally with FA (45 mg /kg body weight) dissolved in saline 3 days/week for 4 weeks; OP-treated group-administrated orally with OP (40 mg /kg body weight) dissolved in corn oil 3 days/week for 4 weeks; FA + OP-treated group-administrated orally with FA (45 mg /kg body weight) dissolved in saline 3 days/week for 4 weeks then administrated orally with OP (40 mg/kg body weight) dissolved in corn oil 3 days/week for another 4 weeks. The results obtained showed that OP-exposed rats had significant increase in serum aspartate aminotransferase, alanine aminotransferase, γ-glutamyl transferase, lactate dehydrogenase, bilirubin, serum and liver tumor necrosis factor-α, interleukin-1β and malondialdehyde, serum interleukin-6 and interleukin-10 and significant decrease in serum alkaline phosphatase, acid phosphatase, serum and liver superoxide dismutase, glutathione peroxidase, and catalase. OP caused an inhibitory action on the gene expression of liver proteins. Rats treated with FA before OP exposure had near-normal values. In addition, FA prevented the degradation of liver deoxyribonucleic acid (DNA), and DNA reformation occurred. In conclusion, FA protects from dangerous OP-related hepatic effects, and these results were supported by molecular and histological investigations.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8d3feacb56a7b2bdad9a4741ed9691aTest
https://doi.org/10.1080/19390211.2018.1443190Test

المؤلفون: Omid Safari, Jasem G. Marammazi, Mansour Torfi Mozanzadeh, Morteza Yaghoubi

المصدر: Fish Physiology and Biochemistry. 44:853-868

مصطلحات موضوعية: Blood Glucose, 0301 basic medicine, Arginine, Physiology, Aquatic Science, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Fish meal, Animal science, Lactate dehydrogenase, Animals, Aspartate Aminotransferases, Essential amino acid, chemistry.chemical_classification, Meal, L-Lactate Dehydrogenase, biology, Superoxide Dismutase, Malnutrition, Alanine Transaminase, Phosphorus, 04 agricultural and veterinary sciences, General Medicine, Metabolism, Alkaline Phosphatase, Animal Feed, Lipids, Diet, Perciformes, Gastrointestinal Tract, 030104 developmental biology, Liver, chemistry, Digestive enzyme, 040102 fisheries, biology.protein, 0401 agriculture, forestry, and fisheries, Alkaline phosphatase, Amino Acids, Essential

الوصف: As amino acids (AAs) are vital molecules in the metabolism of all living organisms and are the building blocks of enzymes, a 6-week feeding trial was conducted for determining the influence of dietary essential amino acid (EAA) deficiencies on pancreatic, plasma, and hepatic enzyme activities in silvery-black porgy (initial weight 4.7 ± 0.01 g) juveniles. Eleven isoproteic (ca. 47%) and isoenergetic (ca. 20.5 MJ kg−1) diets were formulated including a control diet, in which 60% of dietary nitrogen were provided by intact protein (fish meal, gelatin, and wheat meal) and 40% by crystalline AA. The other 10 diets were formulated by 40% reduction in each EAA from the control diet. At the end of the experiment, fish fed with threonine-deficient diet showed the lowest survival rate (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e022d44be5bc321c147fff522dfb5051Test
https://doi.org/10.1007/s10695-018-0475-3Test