المؤلفون: Laiba, Arshad, Alamgeer, Hafiz Muhammad, Irfan, Nabeela, Noor, Nasser Hadal, Alotaibi, Khalid, Saad Alharbi, Syed Nasir, Abbas Bukhari, Yusuf S, Althobaiti, Aman, Ullah, Sher Wali, Khan

المصدر: Pakistan journal of pharmaceutical sciences. 35(1(Supplementary))

مصطلحات موضوعية: Male, Rats, Sprague-Dawley, Thymus Plant, L-Lactate Dehydrogenase, Plant Extracts, Isoproterenol, Myocardial Ischemia, Animals, Female, Aspartate Aminotransferases, Biomarkers, Rats

الوصف: In developing countries, myocardial ischemia and the resulting impairments in heart function are the leading cause of illness and mortality. Thymus linearis Benth has been used as an antibiotic, antioxidant, and antihypertensive agent for centuries. The goal of this investigation was to see if Thymus linearis could protect isoproterenol and doxorubicin-induced myocardial ischemia in vivo at doses of 25 mg/kg s.c. and 15 mg/kg i.p., respectively. The level of cardiac enzymes (CK-MB, LDH, and AST) in the serum isolated from the experimental animal's blood was used to determine myocardial ischemia. The anti-ischemic potential was assessed by comparing the levels of the aforementioned cardiac biomarkers in the intoxicated and treated animal groups. The study found substantial increase (p0.0001) in the serum levels of CK-MB, LDH, AST when compared to intoxicated groups, while pretreatment of animals with crude extract of Thymus linearis significantly reduced the rise in serum cardiac indicators. The findings of the study indicated that the aqueous methanolic Thymus linearis crude extract has cardioprotective potential against Isoproterenol and Doxorubicin-induced cardiac necrosis in rats.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::bc7c288052a1ef260b80f1f6950de823Test
https://pubmed.ncbi.nlm.nih.gov/35228189Test

المؤلفون: Yuan Yuan, Shaoxiang Li, Wei Zhang, Gehong Dong, Xue He, Yuhua Zhang, Chunyan Guan, Zi-Fen Gao, Jia Li, Xue Li

المصدر: American Journal of Infection Control

مصطلحات موضوعية: Leukocytosis, Epidemiology, Coronary Disease, Comorbidity, Disease, Cochrane Library, Severity of Illness Index, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, 030212 general & internal medicine, Creatine Kinase, 0303 health sciences, musculoskeletal, neural, and ocular physiology, Health Policy, Smoking, Alanine Transaminase, Prognosis, Intensive Care Units, C-Reactive Protein, Infectious Diseases, Creatinine, Meta-analysis, Hypertension, Procalcitonin, Cohort study, medicine.medical_specialty, Fever, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Pulmonary disease, macromolecular substances, Article, 03 medical and health sciences, Lymphopenia, Internal medicine, Diabetes Mellitus, medicine, Humans, Aspartate Aminotransferases, Clinical characteristics, L-Lactate Dehydrogenase, SARS-CoV-2, 030306 microbiology, business.industry, ICU cases, Public Health, Environmental and Occupational Health, COVID-19, medicine.disease, Thrombocytopenia, Cerebrovascular Disorders, Pneumonia, Dyspnea, nervous system, Severe illness, business

الوصف: Highlights • Smoking was significantly associated with severe COVID-19 disease. • Patients with severe disease or ICU patients were more likely to have comorbidities. • Fever and dyspnea were significantly associated with severe COVID-19 disease. • Patients with severe disease had higher mortality rates and lower discharge rates.
Objective We aimed to investigate the relationship between clinical characteristics, outcomes and the severity of severe acute respiratory syndrome coronavirus 2 pneumonia. Methods We performed a systematic review and meta-analysis using PubMed, Embase, and Cochrane Library databases to assess the clinical characteristics and outcomes of confirmed COVID-19 cases and compared severe (ICU) and nonsevere (non-ICU) groups. Results We included 12 cohort studies including 2,445 patients with COVID-19. Compared with nonsevere (non-ICU) patients, severe (ICU) disease was associated with a smoking history (P = .003) and comorbidities including chronic obstructive pulmonary disease (OR = 5.08, P < .001), diabetes (OR = 3.17, P < .001), hypertension (OR = 2.40, P < .001), coronary heart disease (OR = 2.66, P < .001), cerebrovascular diseases (OR = 2.68, P = .008), and malignancy (OR=2.21, P = .040). We found significant differences between the 2 groups for fever, dyspnea, decreased lymphocyte and platelet counts, and increased leukocyte count, C-creative protein, procalcitonin, lactose dehydrogenase, aspartate aminotransferase, alanine aminotransferase, creatinine kinase, and creatinine levels (P < .05). Significant differences were also observed for multiple treatments (P < .05). Patients in the severe (ICU) group were more likely to have complications and had a much higher mortality rate and lower discharge rate than those with nonsevere (non-ICU) disease (P < .05). Conclusions Investigation of clinical characteristics and outcomes of severe cases of COVID-19 will contribute to early prediction, accurate diagnosis, and treatment to improve the prognosis of patients with severe illness.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6208437e113dcb1839d9268f8c676bc2Test
https://doi.org/10.1016/j.ajic.2020.06.008Test

المؤلفون: Haoxuan Ding, Jie Feng, Qian Zhang, Lingjun Chen, Xiaonan Yu, Zhonghang Wang

المصدر: Metallomics : integrated biometal science. 13(10)

مصطلحات موضوعية: Male, medicine.medical_specialty, Iron Overload, Biophysics, medicine.disease_cause, Biochemistry, Biomaterials, Superoxide dismutase, Lipid peroxidation, Bile Acids and Salts, chemistry.chemical_compound, Mice, Lactate dehydrogenase, Internal medicine, Lipidomics, medicine, Animals, Aspartate Aminotransferases, chemistry.chemical_classification, Liver injury, Reactive oxygen species, biology, L-Lactate Dehydrogenase, Superoxide Dismutase, Metals and Alloys, Alanine Transaminase, medicine.disease, Lipids, Lipoproteins, LDL, Mice, Inbred C57BL, Endocrinology, chemistry, Liver, Chemistry (miscellaneous), biology.protein, Lipid Peroxidation, Lipoproteins, HDL, Reactive Oxygen Species, Oxidative stress, Lipoprotein

الوصف: Iron overload is an important contributor to disease. The liver, the major site of iron storage in the body, is a key organ impacted by iron overload. While several studies have reported perturbations in liver lipids in iron overload, it is not clear, on a global scale, how individual liver lipid ions are altered. Here, we used lipidomics to study the changes in hepatic lipid ions in iron-overloaded mice. Iron overload was induced by daily intraperitoneal injections of 100 mg/kg body weight iron dextran for 1 week. Iron overload was verified by serum markers of iron status, liver iron quantitation, and Perls stain. Compared with the control group, the serum of iron-overload mice exhibited low levels of urea nitrogen and high-density lipoprotein (HDL), and high concentrations of total bile acid, low-density lipoprotein (LDL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH), suggestive of liver injury. Moreover, iron overload disrupted liver morphology, induced reactive oxygen species (ROS) production, reduced superoxide dismutase (SOD) activity, caused lipid peroxidation, and led to DNA fragmentation. Iron overload altered the overall composition of lipid ions in the liver, with significant changes in over 100 unique lipid ions. Notably, iron overload selectively increased the overall abundance of glycerolipids and changed the composition of glycerophospholipids and sphingolipids. This study, one of the first to report iron-overload induced lipid alterations on a global lipidomics scale, provides early insight into lipid ions that may be involved in iron overload-induced pathology.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41c01a2398d704b4bf2df15125b92691Test
https://pubmed.ncbi.nlm.nih.gov/34562083Test

المؤلفون: Alberto Estirado, J. Ignacio Serrano, Julián Benito-León, Ma. Dolores del Castillo, Souvik Dubey, Ritwik Ghosh

المصدر: Journal of Medical Internet Research
Journal of Medical Internet Research, Vol 23, Iss 5, p e25988 (2021)

مصطلحات موضوعية: 0301 basic medicine, Longitudinal study, Neutrophils, detection, severity, Datasets as Topic, data set, Cell Count, Monocytes, chemistry.chemical_compound, 0302 clinical medicine, Cluster Analysis, characterization, Longitudinal Studies, Lymphocytes, intervention, biology, emergency, Alanine Transaminase, Prognosis, testing, machine learning, C-Reactive Protein, outcome, Unsupervised learning, Public aspects of medicine, RA1-1270, Emergency Service, Hospital, medicine.medical_specialty, Computer applications to medicine. Medical informatics, R858-859.7, Aspartate transaminase, Health Informatics, 03 medical and health sciences, Internal medicine, Lactate dehydrogenase, medicine, Humans, Aspartate Aminotransferases, Original Paper, L-Lactate Dehydrogenase, business.industry, subgroup, COVID-19, Emergency department, Triage, 030104 developmental biology, Alanine transaminase, chemistry, Spain, biology.protein, Observational study, business, 030217 neurology & neurosurgery, Unsupervised Machine Learning

الوصف: Background Early detection and intervention are the key factors for improving outcomes in patients with COVID-19. Objective The objective of this observational longitudinal study was to identify nonoverlapping severity subgroups (ie, clusters) among patients with COVID-19, based exclusively on clinical data and standard laboratory tests obtained during patient assessment in the emergency department. Methods We applied unsupervised machine learning to a data set of 853 patients with COVID-19 from the HM group of hospitals (HM Hospitales) in Madrid, Spain. Age and sex were not considered while building the clusters, as these variables could introduce biases in machine learning algorithms and raise ethical implications or enable discrimination in triage protocols. Results From 850 clinical and laboratory variables, four tests—the serum levels of aspartate transaminase (AST), lactate dehydrogenase (LDH), C-reactive protein (CRP), and the number of neutrophils—were enough to segregate the entire patient pool into three separate clusters. Further, the percentage of monocytes and lymphocytes and the levels of alanine transaminase (ALT) distinguished cluster 3 patients from the other two clusters. The highest proportion of deceased patients; the highest levels of AST, ALT, LDH, and CRP; the highest number of neutrophils; and the lowest percentages of monocytes and lymphocytes characterized cluster 1. Cluster 2 included a lower proportion of deceased patients and intermediate levels of the previous laboratory tests. The lowest proportion of deceased patients; the lowest levels of AST, ALT, LDH, and CRP; the lowest number of neutrophils; and the highest percentages of monocytes and lymphocytes characterized cluster 3. Conclusions A few standard laboratory tests, deemed available in all emergency departments, have shown good discriminative power for the characterization of severity subgroups among patients with COVID-19.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1e63dc2bfc8810481a3d50d0f56f8e8Test
http://europepmc.org/articles/PMC8163491Test

المؤلفون: Yusuke Imamura, Hideo Fujita, Yoshiyuki Morishita, Koichi Yuri, Kenji Matsumoto, Naoyuki Kimura, Yosuke Taniguchi, Akio Matsuda, Yohei Nomura, Daijiro Hori, Akinori Aomatsu, Atsushi Yamaguchi

المصدر: The American Journal of Cardiology. 128:35-44

مصطلحات موضوعية: Male, medicine.medical_specialty, Transcatheter aortic, 030204 cardiovascular system & hematology, Leukocyte Counts, Creatine, Transcatheter Aortic Valve Replacement, Leukocyte Count, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, Aortic valve replacement, Internal medicine, medicine, Creatine Kinase, MB Form, Humans, Aspartate Aminotransferases, Hospital Mortality, Perioperative Period, Creatine Kinase, Aged, Aged, 80 and over, Heart Valve Prosthesis Implantation, L-Lactate Dehydrogenase, biology, business.industry, 030208 emergency & critical care medicine, Aortic Valve Stenosis, Perioperative, medicine.disease, Stenosis, chemistry, Case-Control Studies, biology.protein, Cardiology, Cytokines, Female, Creatine kinase, Cardiology and Cardiovascular Medicine, business

الوصف: The immune response after transcatheter aortic valve implantation (TAVI) in comparison to that after surgical aortic valve replacement (SAVR) remains to be fully elucidated. In a 2-part study, we assessed laboratory data obtained before, immediately after, and 24 and 48 hours after SAVR (128 patients; age ≥80 [mean 82] years) or transfemoral TAVI (102 patients; age ≥80 [mean 86] years) performed for aortic stenosis. In-hospital mortalities were similar (3% vs 0%), but leukocyte counts and aspartate aminotransferase and creatine kinas concentrations were decreased immediately and 24 hours after surgery (all, p0.001). We performed cytokine profiling in a SAVR group (11 patients; mean age, 77 years) and transfemoral TAVI group (12 patients; mean age, 84 years). By measuring normalized concentrations of 71 cytokines at 3 time points, we found a significant difference (defined as fold change1.7 and p0.05 [by Mann-Whitney U-test]) in 23 cytokines. The differentially expressed cytokines fell into 3 hierarchical clusters: cluster A (high increase after SAVR and suppressed increase after TAVI only immediately after surgery [CCL2, CCL4, and 2 others]), cluster B (high increase after SAVR and suppressed increase after TAVI at 2 time points [IL-1Ra, IL-6, IL-8, IL-10, and 5 others]), and cluster C (various patterns [TRAIL, CCL11, and 8 others]). Gene enrichment analysis identified multiple pathways associated with the inflammatory responses in SAVR and altered responses in TAVI, including cellular responses to tumor necrosis factor (p = 0.0035) and interleukin-1 (p = 0.0062). In conclusion, a robust inflammatory response follows SAVR, and a comparatively attenuated response follows TAVI.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bed7d5ec012f195f3459adbb58fcd347Test
https://doi.org/10.1016/j.amjcard.2020.04.037Test

المؤلفون: Valeriy A Sedelev, Anatoliy P Tsyganov, Gulnaz E Sadykanova, Anna V Egorina, Kulzipa Zh Dakieva, Anatoliy S Chursin, Anargul S Sharipkhanova

المصدر: Toxicology and Industrial Health. 36:591-603

مصطلحات موضوعية: Adult, Male, 030213 general clinical medicine, Health, Toxicology and Mutagenesis, Occupational disease, chemistry.chemical_element, Toxicology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Occupational Exposure, Lactate dehydrogenase, medicine, Animals, Humans, Magnesium, Aspartate Aminotransferases, Rats, Wistar, Workplace, Cholinesterase, Titanium, biology, 0402 animal and dairy science, Public Health, Environmental and Occupational Health, Alanine Transaminase, gamma-Glutamyltransferase, 04 agricultural and veterinary sciences, Middle Aged, medicine.disease, 040201 dairy & animal science, Kazakhstan, Work environment, Rats, Male workers, chemistry, biology.protein, Alkaline phosphatase, Female

الوصف: This study explored biochemical parameters of blood in workers operating in the main workshops at the Ust-Kamenogorsk Titanium and Magnesium Plant: magnesium (Mg) workshop (furnace operator, chlorinator operator, and electrolyzer operator), titanium tetrachloride workshop (mill operator and titanium (Ti) production operator), and Ti sponge workshop (reduction furnace operator, knockout operator, and crushing machine operator). The control group consisted of 112 male workers, whose duties were not related to similar occupational hazards (plumbers, electricians, janitors cleaning the administrative building, security guards, and carpenters). The activity of gamma-glutamyl transferase, aspartate aminotransferase, alanine aminotransferase, creatine phosphokinase, lactate dehydrogenase, cholinesterase, alkaline phosphate, and α-amylase and the concentration of serum calcium, Mg, phosphorus, and chloride ion were measured. To clarify the nature of pathological changes that occur in the body of mammals under the influence of toxic gases and dust, this study also included 130 sexually mature, white female rats. Animals and workers were exposed to examination of the same indicators. Changes in test results of enzymes and minerals indicated a negative impact that harmful production factors may have had on the bodies of workers. Findings showed significant fluctuations in enzyme and mineral blood profiles of workers as compared to controls. In the test animals, changes in the enzyme activity and mineral blood composition were as diverse as in the workers. These findings will be useful when identifying markers of a negative impact of harmful substances in an industrial workplace and when developing measures to prevent employees from developing an occupational disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f44ea988c3ee197f42d58a406edc6918Test
https://doi.org/10.1177/0748233720941731Test

المؤلفون: E Ahmed, Esraa A. Ahmed, Ahmed M. Abd-Eldayem

المصدر: Humanexperimental toxicology. 40(10)

مصطلحات موضوعية: Health, Toxicology and Mutagenesis, medicine.medical_treatment, Liver transplantation, Pharmacology, Toxicology, Nitric Oxide, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, Malondialdehyde, Granulocyte Colony-Stimulating Factor, medicine, Animals, Aspartate Aminotransferases, Hepatoprotective Agent, Rats, Wistar, Acetaminophen, L-Lactate Dehydrogenase, business.industry, Liver cell, digestive, oral, and skin physiology, Alanine Transaminase, General Medicine, Glutathione, Free Radical Scavengers, Analgesics, Non-Narcotic, Alkaline Phosphatase, Catalase, Acetylcysteine, Rats, chemistry, Gene Expression Regulation, 030220 oncology & carcinogenesis, Alkaline phosphatase, 030211 gastroenterology & hepatology, Female, Chemical and Drug Induced Liver Injury, business, medicine.drug

الوصف: Acetaminophen (APAP) is often used as an antipyretic and analgesic agent. Overdose hepatotoxicity, which often results in liver cell failure and liver transplantation, is a severe complication of APAP usage. To save the liver and save lives from acute liver damage caused by APAP, the search for new strategies for liver defense is important. Wistar rats have been used for the induction of APAP hepatotoxicity. Elevated levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were evaluated for liver toxicity. In addition, the levels of hepatic tissue oxidative markers such as malondialdehyde (MDA), nitric oxide (NO) increased while glutathione (GSH) was depleted and catalase (CAT) activity was curtailed. The biochemical findings were consistent with the changes in histology that suggested liver damage and inflammation. Treated rats with N-acetylcysteine (N-AC) and granulocyte colony stimulating factor (G-CSF) showed a decrease in serum levels of ALT, AST and LDH, while the level of ALP in the G-CSF group was still high. After administration of APAP, treatment with N-AC or G-CSF substantially reduced the level of MDA and NO while maintaining the GSH content and CAT activity. Treatment with N-AC and G-CSF after administration of APAP has also attenuated inflammation and hepatocytes necrosis. The results of this study showed that G-CSF could be viewed as an alternative hepatoprotective agent against APAP-induced acute liver injury compared to N-AC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d489d94b519181297e3ce2d5c936f841Test
https://pubmed.ncbi.nlm.nih.gov/33882750Test

المؤلفون: Haitao Yang, Enhua Xiao, Jiayi Liu, Qizhi Yu, Deng Huang, Juan Chen, Yeyu Cai, Taili Chen, Xiangyu Chen, Cong Ma, Zhu Chen, Quan-Liang Shang

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)

مصطلحات موضوعية: Adult, Male, China, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Science, Diseases, Gastroenterology, Article, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, chemistry.chemical_compound, Medical research, 0302 clinical medicine, Internal medicine, White blood cell, Lactate dehydrogenase, medicine, Humans, Aspartate Aminotransferases, 030212 general & internal medicine, Alanine aminotransferase, Disease prognosis, Retrospective Studies, Multidisciplinary, L-Lactate Dehydrogenase, business.industry, Significant difference, COVID-19, Alanine Transaminase, Retrospective cohort study, Middle Aged, Prognosis, medicine.disease, Patient Discharge, Pneumonia, medicine.anatomical_structure, chemistry, Medicine, Female, Tomography, X-Ray Computed, business

الوصف: To determine the correlation between the clinical, laboratory, and radiological findings and the hospitalization days in Coronavirus Infectious Disease-19 (COVID-19) discharged patients. We retrospectively identified 172 discharged patients with COVID-19 pneumonia from January 10, 2020, to February 28, 2020, in Hunan province. The patients were categorized into group 1 (≤ 19 days) and group 2 (> 19 days) based on the time from symptom onset to discharge. Cough during admission occurred more commonly in group 2 (68.4%) than in group 1 (53.1%, p = 0.042). White blood cell (p = 0.045), neutrophil counts (p = 0.023), Alanine aminotransferase (p = 0.029), Aspartate aminotransferase (p = 0.027) and Lactate dehydrogenase (p = 0.021) that were above normal were more common in group 2. Patients with single lesions were observed more in group 1(17.7%, p = 0.018) and multiple lesions observed more in group 2(86.8%, p = 0.012). The number of lobes involved (p = 0.008) in the CT score (p = 0.001) for each patient was all differences between the two groups with a statistically significant difference. Mixed ground-glass opacity (GGO) and consolidation appearances were observed in most patients. GGO components > consolidation appearance was more common in group 1 (25.0%) than in group 2 (8.0%) with a significant difference (0.015), GGO p = 0.012). From the logistic regression analysis, the CT score (OR, 1.223; 95% CI, 1.004 to 1.491, p = 0.046) and the appearance of GGO > consolidation (OR, 0.150; 95% CI, 0.034 to 0.660, p = 0.012) were independently associated with the hospitalization days. Thus, special attention should be paid to the role of radiological features in monitoring the disease prognosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07b3f68b7635c82dc071fff2e404262aTest
https://doi.org/10.1038/s41598-021-83654-7Test

المؤلفون: Qi Yang, Shujing Huang, Ran Han, Bo Lin, Qinglin Liu, Xueguang Duan, Zikun Ma, Huiyun Zhang, Haochang Shou, Shengwei Zhang

المصدر: Annals of palliative medicine. 11(4)

مصطلحات موضوعية: Advanced and Specialized Nursing, Hemoglobins, Anesthesiology and Pain Medicine, L-Lactate Dehydrogenase, Humans, Bilirubin, Aspartate Aminotransferases, Hemolysis

الوصف: This study verified and assessed 26 biochemical indicators tested by a dry chemistry analyzer using the hemolytic index test function to determine the degree of interference and the trends among the hemolysis samples on the test results. This study also sought to ensure that reasonable test reports could be issued taking into account practical clinical needs.The samples were manually divided into the control group and the test group. The hemolytic index and biochemical indicators of the samples were tested using the Ortho Vitros 5600 to compare the deviation of the test results between the 2 groups. The judgment standard was set as 1/3 of the total error allowable as required by the quality assessment criterion of the National Center for Clinical Laboratories. The interference degree of hemolysis on the dry chemistry-based biochemical indicators was assessed, and the hemolytic thresholds of 26 biochemical indicators provided by the manufacturer were verified in terms of their validity and rationality.The hemolytic thresholds of 26 dry chemistry-based biochemical indicators were verified to analyze the degree of interference. The results revealed that hemolysis interfered with 17 indicators. Hemolysis positively interfered with the test results of phosphorus, creatine kinase, gamma glutamyl transpeptidase (γ-GGT), magnesium, iron, total protein, potassium, total bilirubin, lactate dehydrogenase, albumin, and aspartate aminotransferase, but negatively interfered with cholinesterase, direct high-density lipoprotein cholesterol, glucose, elevated carbon dioxide alkaline phosphatase, and alanine aminotransferase. A negative deviation of γ-GGT by hemoglobin was described in the manufacturer's statement, but our test data showed a positive deviation by hemolysis. The hemolytic threshold verification results of the other biochemical indicators were consistent with the manufacturer's statement.The hemolytic index test function was used to determine which samples were interfered with by hemolysis to make an analytical judgment according to the hemolytic interference thresholds of the different test items, verify the validity of the hemolytic thresholds of the test items, perform reasonable tests on the hemolytic samples, and issue valid reports to reduce the rejection rate of the hemolytic samples, shorten the turnaround time (TAT) of laboratories.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c81d14a5dc866c117b18c19e9c478e7Test
https://pubmed.ncbi.nlm.nih.gov/35523746Test

المؤلفون: Omar Benzakour, Adriana Delwail, Sébastien Giraud, Sandrine Joffrion, Thierry Hauet, Thomas Pelé, Jean-Michel Goujon, Laurent Macchi, Virginie Ameteau, Fatima Dkhissi

المساهمون: Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques ( IRTOMIT), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Poitiers (CHU Poitiers), Université de Poitiers - Faculté de Médecine et de Pharmacie, Université de Poitiers, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Pinet, Nicolas

المصدر: International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2021, 22 (22), pp.12103. ⟨10.3390/ijms222212103⟩
International Journal of Molecular Sciences, 2021, 22 (22), pp.12103. ⟨10.3390/ijms222212103⟩
Volume 22
Issue 22
International Journal of Molecular Sciences, Vol 22, Iss 12103, p 12103 (2021)

مصطلحات موضوعية: Nitric Oxide Synthase Type II, 030204 cardiovascular system & hematology, Kidney, Receptor tyrosine kinase, ischemia-reperfusion, chemistry.chemical_compound, 0302 clinical medicine, Biology (General), Spectroscopy, Chemokine CCL2, microparticles, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, biology, phagocytosis, General Medicine, Acute Kidney Injury, Apoptotic body, Computer Science Applications, Chemistry, medicine.anatomical_structure, Myeloperoxidase, Creatinine, Reperfusion Injury, RCS rats, medicine.medical_specialty, QH301-705.5, Nitric Oxide, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, MerTK, Lipocalin-2, Internal medicine, medicine, Animals, Humans, Aspartate Aminotransferases, Physical and Theoretical Chemistry, QD1-999, Molecular Biology, 030304 developmental biology, Peroxidase, Renal ischemia, L-Lactate Dehydrogenase, c-Mer Tyrosine Kinase, business.industry, Macrophages, Organic Chemistry, MERTK, medicine.disease, Rats, Endocrinology, chemistry, inflammation, biology.protein, business, Reperfusion injury, Cell Adhesion Molecules, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience; Renal ischaemia reperfusion (I/R) triggers a cascade of events including oxidative stress, apoptotic body and microparticle (MP) formation as well as an acute inflammatory process that may contribute to organ failure. Macrophages are recruited to phagocytose cell debris and MPs. The tyrosine kinase receptor MerTK is a major player in the phagocytosis process. Experimental models of renal I/R events are of major importance for identifying I/R key players and for elaborating novel therapeutical approaches. A major aim of our study was to investigate possible involvement of MerTK in renal I/R. We performed our study on both natural mutant rats for MerTK (referred to as RCS) and on wild type rats referred to as WT. I/R was established by of bilateral clamping of the renal pedicles for 30′ followed by three days of reperfusion. Plasma samples were analysed for creatinine, aspartate aminotransferase (ASAT), lactate dehydrogenase (LDH), kidney injury molecule -1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) levels and for MPs. Kidney tissue damage and CD68-positive cell requirement were analysed by histochemistry. monocyte chemoattractant protein-1 (MCP-1), myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS), and histone 3A (H3A) levels in kidney tissue lysates were analysed by western blotting. The phagocytic activity of blood-isolated monocytes collected from RCS or WT towards annexin-V positive bodies derived from cultured renal cell was assessed by fluorescence-activated single cell sorting (FACS) and confocal microscopy analyses. The renal I/R model for RCS rat described for the first time here paves the way for further investigations of MerTK-dependent events in renal tissue injury and repair mechanisms.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a19f686ea9beaf070d18f312cff0e2a8Test
https://hal.archives-ouvertes.fr/hal-03468386Test