Anti-inflammatory therapies in TRAMP mice: delay in PCa progression
| العنوان: | Anti-inflammatory therapies in TRAMP mice: delay in PCa progression |
|---|---|
| المؤلفون: | Ronaldo A. Pilli, Elaine Minatel, Fabio Montico, Aline Barbosa Macedo, Valéria Helena Alves Cagnon, Rafael Sauce, Larissa Akemi Kido, João Carvalho, Débora Barbosa Vendramini Costa |
| المساهمون: | UNIVERSIDADE ESTADUAL DE CAMPINAS |
| المصدر: | Repositório da Produção Científica e Intelectual da Unicamp Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, STAT3 Transcription Factor, Cancer Research, Endocrinology, Diabetes and Metabolism, Interleukin-1beta, Anti-Inflammatory Agents, Antineoplastic Agents, Apoptosis, Mice, Transgenic, Adenocarcinoma, Receptor, IGF Type 1, 03 medical and health sciences, Prostate cancer, Mice, 0302 clinical medicine, Endocrinology, Goniotalamina, Prostate, Proliferating Cell Nuclear Antigen, medicine, Animals, Artigo original, Intraepithelial neoplasia, business.industry, Tumor Necrosis Factor-alpha, NF-kappa B, Cancer, Prostatic Neoplasms, medicine.disease, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Oncology, Celecoxib, Cyclooxygenase 2, Pyrones, 030220 oncology & carcinogenesis, Aza-goniothalamin, Cancer research, Immunohistochemistry, Prostatic neoplasms, business, medicine.drug, Tramp, Neoplasias da próstata |
| الوصف: | The aim of this study was to characterize the structural and molecular biology as well as evaluate the immediate and late responses of prostatic cancer in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model after treatment with goniothalamin (GTN) and celecoxib. The treated mice received GTN (150 mg/kg, gavage) or celecoxib (10 mg/kg, gavage) from 8 to 12 weeks of age. They were killed at different ages: the immediate-response groups at 12 weeks and the late-response groups at 22 weeks. The ventral prostate was collected for light microscopy, immunohistochemistry, western blotting, TUNEL, and ELISA. Morphological analyses indicated that GTN treatment delayed the progression of prostatic adenocarcinoma, leading to a significant decrease of prostatic lesion frequency in both experimental period responses to this treatment, mainly high-grade prostatic intraepithelial neoplasia and well-differentiated adenocarcinoma. Also, the celecoxib treatment showed a particular decrease in the proliferative processes (PCNA) in both the experimental periods. Despite celecoxib diminishing the COX2 and IGFR1 levels, GTN presented higher action spectrum considering the decrease of a greater molecular number involved in the proliferative and inflammatory processes in prostatic cancer. Goniothalamin attenuated the pro-inflammatory response in TRAMP prostatic microenvironment, delaying prostate cancer (PCa) progression. Celecoxib treatment was efficient in the regulation of COX2 in the TRAMP mice, mainly in the advanced disease grade. Finally, we concluded that inflammatory process control in early grades of PCa was crucial for the downregulation of the signaling pathways involved in the proliferative processes in advanced cancer grades FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP Fechado |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caaac219484e565ea912a7d5b4e5fff4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....caaac219484e565ea912a7d5b4e5fff4 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |