دورية أكاديمية
Allantoin in human plasma, serum, and nasal-lining fluids as a biomarker of oxidative stress: Avoiding artifacts and establishing real in vivo concentrations
| العنوان: | Allantoin in human plasma, serum, and nasal-lining fluids as a biomarker of oxidative stress: Avoiding artifacts and establishing real in vivo concentrations |
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| المؤلفون: | Lee, CYJ, Gruber, J, Tang, SY, Jenner, AM, Mudway, I, Blomberg, A, Behndig, A, Kasiman, K, Seet, RCS, Zhang, W, Chen, C, Kelly, FJ, Halliwell, B |
| بيانات النشر: | //www.liebertpub.com/ars United States |
| سنة النشر: | 2009 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Adult, Aged, 80 And Over, Allantoin - Blood, Artifacts, Biological Markers - Blood, Body Fluids - Chemistry, Cross-Over Studies, Double-Blind Method, Female, Gas Chromatography-Mass Spectrometry, Humans, Oxidative Stress, Nasal Mucosa - Secretion, Middle Aged, Male, Isoprostanes - Analysis |
| الوصف: | Urate is the terminal product of purine metabolism in primates, including humans. Urate is also an efficient scavenger of oxidizing species and is thought to be an important antioxidant in human body fluids. Allantoin, the major oxidation product of urate, has been suggested as a candidate biomarker of oxidative stress because it is not produced metabolically. Although urate is converted to allantoin under strongly alkaline pH, such conditions have been used in the past to facilitate extraction of allantoin. We evolved a method for the determination of allantoin concentrations in human plasma and serum by gas chromatography-mass spectrometry without such artifact. With this method, we show that alkaline conditions do indeed cause breakdown of urate, leading to significant overestimation of allantoin concentration in human samples. By using our alternative method, serum samples from 98 volunteers were analyzed, and allantoin levels were found to be significantly lower than was previously reported. The in vivo utility and sensitivity of our method was further evaluated in human nasal-lining fluids. We were able to demonstrate an ozone-induced increase in allantoin, in the absence of increases in either ascorbate or glutathione oxidation products. © Copyright 2009, Mary Ann Liebert, Inc. 2009. ; published_or_final_version |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1523-0864 |
| العلاقة: | Antioxidants and Redox Signaling; http://www.scopus.com/mlt/select.url?eid=2-s2.0-67650239461&selection=ref&src=s&origin=recordpageTest; Antioxidants And Redox Signaling, 2009, v. 11 n. 8, p. 1767-1776; 1776; WOS:000267488900001; eid_2-s2.0-67650239461; 1767; http://hdl.handle.net/10722/179142Test; 11 |
| DOI: | 10.1089/ars.2008.2364 |
| الإتاحة: | https://doi.org/10.1089/ars.2008.2364Test http://hdl.handle.net/10722/179142Test |
| حقوق: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. ; This is a copy of an article published in the [Antioxidants and Redox Signaling] © [2009] [copyright Mary Ann Liebert, Inc.]; [Antioxidants and Redox Signaling] is available online at: http://www.liebertonline.comTest. |
| رقم الانضمام: | edsbas.136434B |
| قاعدة البيانات: | BASE |
| تدمد: | 15230864 |
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| DOI: | 10.1089/ars.2008.2364 |