TRAPP II Complex Assembly Requires Trs33 or Trs65
| العنوان: | TRAPP II Complex Assembly Requires Trs33 or Trs65 |
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| المؤلفون: | Zhanna Lipatova, Andrei A. Tokarev, Geetanjali Sundaram, Nava Segev, Yongheng Liang, David Taussig, Jonathan W. Mulholland |
| المصدر: | Traffic. 10:1831-1844 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Models, Molecular, Saccharomyces cerevisiae Proteins, Protein subunit, Molecular Sequence Data, Vesicular Transport Proteins, Plasma protein binding, Biochemistry, Article, symbols.namesake, Structural Biology, Two-Hybrid System Techniques, Genetics, Amino Acid Sequence, Molecular Biology, Peptide sequence, chemistry.chemical_classification, Sequence Homology, Amino Acid, biology, Cell Biology, Golgi apparatus, Yeast, Amino acid, Cell biology, TRAPP complex, chemistry, symbols, biology.protein, Rab, Protein Binding |
| الوصف: | TRAPP is a multi-subunit complex that acts as a Ypt/Rab activator at the Golgi apparatus. TRAPP exists in two forms: TRAPP I is comprised of five essential and conserved subunits and TRAPP II contains two additional essential and conserved subunits, Trs120 and Trs130. Previously, we have shown that Trs65, a nonessential fungi-specific TRAPP subunit, plays a role in TRAPP II assembly. TRS33 encodes another nonessential but conserved TRAPP subunit whose function is not known. Here, we show that one of these two subunits, nonessential individually, is required for TRAPP II assembly. Trs33 and Trs65 share sequence, intra-cellular localization and interaction similarities. Specifically, Trs33 interacts genetically with both Trs120 and Trs130 and physically with Trs120. In addition, trs33 mutant cells contain lower levels of TRAPP II and exhibit aberrant localization of the Golgi Ypts. Together, our results indicate that in yeast, TRAPP II assembly is an essential process that can be accomplished by either of two related TRAPP subunits. Moreover, because humans express two Trs33 homologues, we propose that the requirement of Trs33 for TRAPP II assembly is conserved from yeast to humans. |
| تدمد: | 1600-0854 1398-9219 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d424fdb91a154bea60a9c6754b1ca6cTest https://doi.org/10.1111/j.1600-0854.2009.00988.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d424fdb91a154bea60a9c6754b1ca6c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16000854 13989219 |
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