NBM-T-BBX-OS01, Semisynthesized from Osthole, Induced G1 Growth Arrest through HDAC6 Inhibition in Lung Cancer Cells
| العنوان: | NBM-T-BBX-OS01, Semisynthesized from Osthole, Induced G1 Growth Arrest through HDAC6 Inhibition in Lung Cancer Cells |
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| المؤلفون: | Chia-Nan Chen, Chia-Yun Hsu, Sheng-Yung Yu, Chung-Yang Huang, Jih-Tung Pai, Kuo Tai Hua, Meng-Shih Weng, Chiung-Ho Liao |
| المصدر: | Molecules Volume 20 Issue 5 Pages 8000-8019 Molecules, Vol 20, Iss 5, Pp 8000-8019 (2015) |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cyclin-Dependent Kinase Inhibitor p21, Proteasome Endopeptidase Complex, Cyclin E, Cell cycle checkpoint, Lung Neoplasms, Pharmaceutical Science, Down-Regulation, Biology, Histone Deacetylase 6, Hydroxamic Acids, Histone Deacetylases, Article, Analytical Chemistry, lcsh:QD241-441, Cyclin D1, lcsh:Organic chemistry, Coumarins, Cell Line, Tumor, Drug Discovery, NBM-T-BBX-OS01 (TBBX), Humans, HSP90 Heat-Shock Proteins, Physical and Theoretical Chemistry, heat shock protein 90, Cell Proliferation, Organic Chemistry, Cyclin-dependent kinase 2, Cyclin-Dependent Kinase 2, G1 Phase, Cyclin-Dependent Kinase 4, Acetylation, Cell Cycle Checkpoints, Cell cycle, suberoylanilide hydroxamic acid (SAHA), Hsp90, Up-Regulation, Histone Deacetylase Inhibitors, lung cancer, Proteasome, Chemistry (miscellaneous), cell cycle arrest, histone deacetylase, Cancer research, biology.protein, Molecular Medicine, CDK inhibitor |
| الوصف: | Disrupting lung tumor growth via histone deacetylases (HDACs) inhibition is a strategy for cancer therapy or prevention. Targeting HDAC6 may disturb the maturation of heat shock protein 90 (Hsp90) mediated cell cycle regulation. In this study, we demonstrated the effects of semisynthesized NBM-T-BBX-OS01 (TBBX) from osthole on HDAC6-mediated growth arrest in lung cancer cells. The results exhibited that the anti-proliferative activity of TBBX in numerous lung cancer cells was more potent than suberoylanilide hydroxamic acid (SAHA), a clinically approved pan-HDAC inhibitor, and the growth inhibitory effect has been mediated through G1 growth arrest. Furthermore, the protein levels of cyclin D1, CDK2 and CDK4 were reduced while cyclin E and CDK inhibitor, p21Waf1/Cip1, were up-regulated in TBBX-treated H1299 cells. The results also displayed that TBBX inhibited HDAC6 activity via down-regulation HDAC6 protein expression. TBBX induced Hsp90 hyper-acetylation and led to the disruption of cyclin D1/Hsp90 and CDK4/Hsp90 association following the degradation of cyclin D1 and CDK4 proteins through proteasome. Ectopic expression of HDAC6 rescued TBBX-induced G1 arrest in H1299 cells. Conclusively, the data suggested that TBBX induced G1 growth arrest may mediate HDAC6-caused Hsp90 hyper-acetylation and consequently increased the degradation of cyclin D1 and CDK4. |
| وصف الملف: | application/pdf |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad1b59c429365a0ae74d9e130199a3d7Test https://pubmed.ncbi.nlm.nih.gov/25946558Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad1b59c429365a0ae74d9e130199a3d7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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