المؤلفون: Carolina Camacho-Fernández, Jose M. Seguí-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martínez

المصدر: Frontiers in Plant Science, Vol 12 (2021)

مصطلحات موضوعية: androgenesis, arabinogalactan proteins, callose, cell wall, cellulose, microspore embryogenesis, Plant culture, SB1-1110

الوصف: Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic structures [compact callus (CC) and loose callus (LC) structures]. Little is known about the factors behind these different responses. In this study we performed a comparative analysis of the composition and architecture of the cell walls of each structure by confocal and quantitative electron microscopy. Each structure presented specific cell wall characteristics that defined their developmental fate. EE and LBS structures, which are responsible for most of the viable embryos, showed a specific profile with thin walls rich in arabinogalactan proteins (AGPs), highly and low methyl-esterified pectin and callose, and a callose-rich subintinal layer not necessarily thick, but with a remarkably high callose concentration. The different profiles of EE and LBS walls support the development as suspensorless and suspensor-bearing embryos, respectively. Conversely, less viable embryogenic structures (LC) presented the thickest walls and the lowest values for almost all of the studied cell wall components. These cell wall properties would be the less favorable for cell proliferation and embryo progression. High levels of highly methyl-esterified pectin are necessary for wall flexibility and growth of highly embryogenic structures. AGPs seem to play a role in cell wall stiffness, possibly due to their putative role as calcium capacitors, explaining the positive relationship between embryogenic potential and calcium levels.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpls.2021.737139/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/2e7aeb004f594c958fd77a086225845dTest

المؤلفون: Jose M. Seguí-Simarro, Carolina Camacho-Fernández, Kim Boutilier, Patricia Corral-Martínez, Ricardo Mir

المصدر: Frontiers in Plant Science, 12
Frontiers in Plant Science 12 (2021)
Frontiers in Plant Science, Vol 12 (2021)
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname

مصطلحات موضوعية: 0106 biological sciences, food.ingredient, Pectin, BIOLOGIA CELULAR, Plant Science, subintinal layer, 01 natural sciences, SB1-1110, Cell wall, 03 medical and health sciences, chemistry.chemical_compound, food, Microspore, Arabinogalactan, Microspore embryogenesis, Subintinal layer, Cell totipotency, BIOS Plant Development Systems, 030304 developmental biology, 0303 health sciences, Cell growth, cell totipotency, Callose, Plant culture, Embryo, cellulose, Cell biology, GENETICA, androgenesis, chemistry, Callus, microspore embryogenesis, cell wall, arabinogalactan proteins, callose, 010606 plant biology & botany

الوصف: [EN] Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic structures [compact callus (CC) and loose callus (LC) structures]. Little is known about the factors behind these different responses. In this study we performed a comparative analysis of the composition and architecture of the cell walls of each structure by confocal and quantitative electron microscopy. Each structure presented specific cell wall characteristics that defined their developmental fate. EE and LBS structures, which are responsible for most of the viable embryos, showed a specific profile with thin walls rich in arabinogalactan proteins (AGPs), highly and low methyl-esterified pectin and callose, and a callose-rich subintinal layer not necessarily thick, but with a remarkably high callose concentration. The different profiles of EE and LBS walls support the development as suspensorless and suspensor-bearing embryos, respectively. Conversely, less viable embryogenic structures (LC) presented the thickest walls and the lowest values for almost all of the studied cell wall components. These cell wall properties would be the less favorable for cell proliferation and embryo progression. High levels of highly methyl-esterified pectin are necessary for wall flexibility and growth of highly embryogenic structures. AGPs seem to play a role in cell wall stiffness, possibly due to their putative role as calcium capacitors, explaining the positive relationship between embryogenic potential and calcium levels.
This work was supported by grant PID2020-115763RBI00 to JS-S from Spanish MICINN and by a Juan de la Cierva -Incorporacion Fellowship and a Marie Sklodowska-Curie Individual Fellowship (656579) to PC-M. RM holds a CDEIGENT (2018/023) fellowship from Generalitat Valenciana.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22d8f6d076a04877691de1cc496c8faaTest
https://doi.org/10.3389/fpls.2021.737139Test