التفاصيل البيبلوغرافية
| العنوان: |
AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in Arabidopsis thaliana. |
| المؤلفون: |
Anabuki, Tomoaki1 (AUTHOR) bukio0119@gmail.com, Ohashi, Keisuke2 (AUTHOR) keiroy124@eis.hokudai.ac.jp, Takasuka, Taichi E.1,2 (AUTHOR) takasuka@cen.agr.hokudai.ac.jp, Matsuura, Hideyuki1 (AUTHOR) matsuura@agr.hokudai.ac.jp, Takahashi, Kosaku3 (AUTHOR) kt207119@nodai.ac.jp |
| المصدر: |
Molecules. Jan2022, Vol. 27 Issue 1, p161. 1p. |
| مصطلحات موضوعية: |
*ARABIDOPSIS proteins, *ABSCISIC acid, *THIOREDOXIN, *CARRIER proteins, *SMALL molecules, *AZIDO group, *BRASSINOSTEROIDS |
| مستخلص: |
Abscisic acid (ABA, 1) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of Arabidopsis thaliana. As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds. [ABSTRACT FROM AUTHOR] |
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