التفاصيل البيبلوغرافية
| العنوان: |
Aptamer-based assay for monitoring genetic disorder phenylketonuria (PKU). |
| المؤلفون: |
Hasanzadeh, Mohammad1 mhmmd_hasanzadeh@yahoo.com, Zargami, Amir2, Baghban, Hossein Navay3, Mokhtarzadeh, Ahad4, Shadjou, Nasrin5, Mahboob, Soltanali2 |
| المصدر: |
International Journal of Biological Macromolecules. Sep2018, Vol. 116, p735-743. 9p. |
| مصطلحات موضوعية: |
*APTAMERS, *PHENYLKETONURIA diagnosis, *GENETIC disorders, *GOLD nanoparticles, *ELECTROCHEMICAL analysis |
| مستخلص: |
The genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine because of a lack of the enzyme phenylalanine hydroxylase. Phenylalanine is used to biochemically form proteins, coded for by DNA. The development of an apta-assay for detection of l -Phenylalanine is presented in this work. A highly specific DNA-aptamer, selected to l -Phenylalanine was immobilized onto a gold nanostructure and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH. We have constructed an aptamer immobilized gold nanostructure mediated, ultrasensitive electrochemical biosensor (Apt/AuNSs/Au electrode) for l -Phenylalanine detection without any additional signal amplification strategy. The aptamer assemble onto the AuNSs makes Apt/AuNSs/Au electrode an excellent platform for the l -Phenylalanine detection in physiological like condition. Differential pulse voltammetry were used for the quantitative l -Phenylalanine detection. The Apt/AuNSs/Au electrode offers an ultrasensitive and selective detection of l -Phenylalanine down to 0.23 μM level with a wide dynamic range from 0.72 μM–6 mM. The aptasensor exhibited excellent selectivity and stability. The real sample analysis was performed by spiking the unprocessed human serum samples with various concentration of l -Phenylalanine and obtained recovery within 2% error value. The sensor is found to be more sensitive than most of the literature reports. The simple and easy way of construction of this apta-assay provides an efficient and promising diagnosis of phenylketonuria. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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