دورية أكاديمية
Regulation of endothelial cell and endothelial progenitor cell survival and vasculogenesis by integrin-linked kinase
| العنوان: | Regulation of endothelial cell and endothelial progenitor cell survival and vasculogenesis by integrin-linked kinase |
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| المؤلفون: | Cho, Hyun-Jai, Youn, Seock-Won, Cheon, Soo-In, Kim, Tae-Youn, Hur, Jin, Zhang, Shu-Ying, Lee, Seung Pyo, Park, Kyung-Woo, Lee, Myoung-Mook, Choi, Yun-Shik, Park, Young-Bae, Kim, Hyo-Soo |
| المساهمون: | 조현재, 윤석원, 천수인, 김태윤, 허진, 이승표, 박경우, 이명묵, 최윤식, 박영배, 김효수 |
| بيانات النشر: | American Heart Association |
| سنة النشر: | 2005 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | Animals, Apoptosis/physiology, Cell Adhesion/physiology, Cell Division/physiology, Cell Survival/physiology, Cells, Cultured, Endothelium, Vascular/*cytology/*enzymology, Female, Gene Expression Regulation, Enzymologic, Gene Transfer Techniques, Humans, Ischemia/metabolism/pathology/physiopathology, Mice, Nude, Neovascularization, Physiologic/*physiology, Protein-Serine-Threonine Kinases/genetics/*metabolism, Signal Transduction/physiology, Stem Cells/*cytology/*enzymology, Umbilical Veins/cytology |
| الوصف: | OBJECTIVE: New vessel formation is a dynamic process of attachment, detachment, and reattachment of endothelial cells (ECs) and endothelial progenitor cells (EPCs) with each other and with the extracellular matrix (ECM). Integrin-linked kinase (ILK) plays a pivotal role in ECM-mediated signaling. Therefore, we investigated the role of ILK in ECs and EPCs during neovascularization. METHODS AND RESULTS: In human umbilical cord vein ECs and EPCs, endogenous ILK expression, along with subsequent cell survival signals phospho-Akt and phospho-glycogen synthase kinase 3beta, was reduced after anchorage or nutrient deprivation. Even brief anchorage deprivation resulted in retarded capillary tube formation by ECs. Adenoviral ILK gene transfer in ECs and EPCs reversed the decrease in cell survival signals after anchorage or nutrient deprivation, leading to enhanced survival, reduced apoptosis, and significantly accelerated the functional recovery after reattachment. And ILK overexpressing EPCs significantly improved blood flow recovery and prevented limb loss in nude mice hindlimb ischemia model. Furthermore, the efficacy of systemic delivery was equivalent to local injection of ILK-EPCs. CONCLUSIONS: ILK overexpression protects ECs and EPCs from anchorage- or nutrient-deprived stress and enhances neovascularization, suggesting that ILK is an optimal target gene for genetically modified cell-based therapy. Neovascularization is a dynamic process of detachment and reattachment of ECs and EPCs. Endogenous ILK expression was decreased in various stress conditions, and the gene transfer of ILK protected ECs and EPCs from temporary anchorage or nutrient deprivation. Furthermore, ILK gene transfer in EPCs significantly enhanced neovascularization in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Arterioscler Thromb Vasc Biol. 2005 Jun;25(6):1154-60. Epub 2005 Mar 31.; 1524-4636 (Electronic); http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15802621Test; https://hdl.handle.net/10371/21390Test |
| DOI: | 10.1161/01.ATV.0000164312.20008.93 |
| الإتاحة: | https://doi.org/10.1161/01.ATV.0000164312.20008.93Test https://hdl.handle.net/10371/21390Test http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15802621Test |
| رقم الانضمام: | edsbas.C33DD055 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1161/01.ATV.0000164312.20008.93 |
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