التفاصيل البيبلوغرافية
| العنوان: |
The NFκB Pathway Inhibitors Bay 11-7082 and Parthenolide Induce Programmed Cell Death in Anucleated Erythrocytes. |
| المؤلفون: |
Ghashghaeinia, Mehrdad, Toulany, Mahmoud, Saki, Mohammad, Bobbala, Diwakar, Fehrenbacher, Birgit, Rupec, Rudolf, Rodemann, H. Peter, Ghoreschi, Kamran, Röcken, Martin, Schaller, Martin, Lang, Florian, Wieder, Thomas |
| المصدر: |
Cellular Physiology & Biochemistry (Karger AG); 2011, Vol. 27 Issue 1, p45-54, 10p |
| مصطلحات موضوعية: |
NF-kappa B, ENZYME inhibitors, ERYTHROCYTES, GENE expression, APOPTOSIS, WESTERN immunoblotting, INTRACELLULAR calcium, GLUTATHIONE, PHOSPHATIDYLSERINES |
| مستخلص: |
The preclinical compounds Bay 11-7082 and parthenolide trigger apoptosis, an effect contributing to their antiinflammatory action. The substances interfere with the activation and nuclear translocation of nuclear factor NFκB, by inhibiting NFκB directly (parthenolide) or by interfering with the inactivation of the NFκB inhibitory protein IκB-α (Bay 11-7082). Beyond that, the substances may be effective in part by nongenomic effects. Similar to apoptosis of nucleated cells, erythrocytes may undergo apoptosis-like cell death (eryptosis) characterized by cell membrane scrambling with phosphatidylserine exposure, and cell shrinkage. Thus, erythrocytes allow the study of nongenomic mechanisms contributing to suicidal cell death, e.g. Ca2+ leakage or glutathione depletion. The present study utilized Western blotting to search for NFκB and IκB-α expression in erythrocytes, FACS analysis to determine cytosolic Ca2+ (Fluo3 fluorescence), phosphatidylserine exposure (annexin V binding), and cell volume (forward scatter), as well as an enzymatic method to determine glutathione levels. As a result, both NFκB and IκB-α are expressed in erythrocytes. Targeting the NFκB pathway by Bay 11-7082 (IC50 ≈ 10 μM) and parthenolide (IC50 ≈ 30 μM) triggered suicidal erythrocyte death as shown by annexin V binding and decrease of forward scatter. Bay 11-7082 treatment further increased intracellular Ca2+ and led to depletion of reduced glutathione. The effects of Bay 11-7082 and parthenolide on annexin V binding could be fully reversed by the antioxidant N-acetylcysteine. In conclusion, the pharmacological inhibitors of NFκB, Bay 11-7082 and parthenolide, interfere with the survival of erythrocytes involving mechanisms other than disruption of NFκB-dependent gene expression. Copyright © 2011 S. Karger AG, Basel [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
