Primary HIV-1 infection sets the stage for important B lymphocyte dysfunctions
| العنوان: | Primary HIV-1 infection sets the stage for important B lymphocyte dysfunctions |
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| المؤلفون: | Kehmia Titanji, Lyda M. Osorio, Francesca Chiodi, Sven Grutzmeier, Chiara Tassandin, Danika Schepis, Giuseppe Tambussi, Lucia Lopalco, Rino Bellocco, Angelo De Milito |
| المساهمون: | Titanji, K, Chiodi, F, Bellocco, R, Schepis, D, Osorio, L, Tassandin, C, Tambussi, G, Grutzmeier, S, Lopalco, L, De Milito, A |
| المصدر: | Karolinska Institutet Scopus-Elsevier |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Male, T-Lymphocytes, Cellular differentiation, Lymphocyte, Apoptosis, HIV Infections, Lymphocyte Activation, Immunoglobulin D, Receptors, Tumor Necrosis Factor, Hypergammaglobulinemia, Antiretroviral Therapy, Highly Active, Immunology and Allergy, HIV Infection, IL-2 receptor, Receptors, Immunologic, Cells, Cultured, B-Lymphocytes, biology, B-Lymphocyte, Cell Differentiation, Middle Aged, Reverse Transcriptase Inhibitor, Antigens, CD95, Phenotype, Infectious Diseases, medicine.anatomical_structure, Reverse Transcriptase Inhibitors, Female, Human, Adult, Adolescent, T cell, Immunology, CD19, Antigens, CD, medicine, Humans, fas Receptor, B cell, Aged, business.industry, Apoptosi, Genes, bcl-2, T-Lymphocyte, Chronic Disease, HIV-1, Leukocytes, Mononuclear, biology.protein, business, Immunologic Memory |
| الوصف: | Objectives: To investigate the effects of primary HIV-1 infection (PHI) and of two antiretroviral therapies [highly active antiretroviral therapy (HAART) or reverse transcriptase inhibitors (RTI)] on activation, differentiation and survival of B cells. Methods: Naive and memory B cells from three groups [PHI (31), chronic infection (26) and healthy donors (12)] were studied for surface expression of Fas, LAIR-1, CD70, intracellular expression of Bcl-2 and spontaneous apoptosis. Fluorescence activated cell sorting (IgD+IgM+CD19+CD27+) and short-term cell culture to analyse induction of CD25 on B cells were performed in five patients with PHI. Patients with PHI were sampled at baseline, and after 1 and 6 months of therapy. Results were analysed by parametric and non-parametric tests and by mathematical modelling. Results: In PHI, B cells were significantly decreased; naive and memory B lymphocytes showed a high degree of activation, manifested by hypergammaglobulinaemia, altered expression of Fas and LAIR-1, and high rate of spontaneous apoptosis. Antiretroviral treatment improved the activation/differentiation status of B cells, reduced apoptosis to levels comparable to those in healthy individuals and restored the ability of B cells to respond to T cell-dependent activation. B cells showed slightly better recovery in patients taking HAART than in those taking RTI. Decreased IgM-positive memory B cells and lower induction of CD25 expression on B cells upon T cell activation at diagnosis of PHI was shown in five patients tested. These parameters normalized after 6 months of therapy. Conclusion: B cell dysfunctions found in chronic HIV-1 infection appear during PHI and initiation of antiretroviral therapy early during infection may help to preserve the B cell compartment. |
| تدمد: | 0269-9370 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d730b57395f7c5758e0d6a30856b0edTest https://doi.org/10.1097/01.aids.0000191231.54170.89Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9d730b57395f7c5758e0d6a30856b0ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02699370 |
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