التفاصيل البيبلوغرافية
| العنوان: |
Quantitative regulation of FLC via coordinated transcriptional initiation and elongation. |
| المؤلفون: |
Zhe Wu1, Ietswaart, Robert1,2, Fuquan Liu1,3, Hongchun Yang1, Howard, Martin1,2 martin.howard@jic.ac.uk, Dean, Caroline1 caroline.dean@jic.ac.uk |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America. 1/5/2016, Vol. 113 Issue 1, p218-223. 6p. |
| مصطلحات موضوعية: |
*LOCUS (Genetics), *FLOWERS, *GENETIC transcription, *ELONGATION factors (Biochemistry), *ARABIDOPSIS thaliana genetics, *ANTISENSE DNA |
| مستخلص: |
The basis of quantitative regulation of gene expression is still poorly understood. In Arabidopsis thaliana, quantitative variation in expression of FLOWERING LOCUS C (FLC) influences the timing of flowering. In ambient temperatures, FLC expression is quantitatively modulated by a chromatin silencing mechanism involving alternative polyadenylation of antisense transcripts. Investigation of this mechanism unexpectedly showed that RNA polymerase II (Pol II) occupancy changes at FLC did not reflect RNA fold changes. Mathematical modeling of these transcriptional dynamics predicted a tight coordination of transcriptional initiation and elongation. This prediction was validated by detailed measurements of total and chromatin-bound FLC intronic RNA, a methodology appropriate for analyzing elongation rate changes in a range of organisms. Transcription initiation was found to vary ~25-fold with elongation rate varying ~8- to 12-fold. Premature sense transcript termination contributed very little to expression differences. This quantitative variation in transcription was coincident with variation in H3K36me3 and H3K4me2 over the FLC gene body. We propose different chromatin states coordinately influence transcriptional initiation and elongation rates and that this coordination is likely to be a general feature of quantitative gene regulation in a chromatin context. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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