Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation
| العنوان: | Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation |
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| المؤلفون: | Mark F. Belmonte, Branimir Gjetvaj, Ronny V. L. Joosen, Kim Boutilier, Andrew G. Sharpe, Chris Maliepaard, Muhammad Tahir, Mohamed Elhiti, Claudio Stasolla, Khalil Khamiss |
| المصدر: | Planta 228 (2008) 2 Planta, 228(2), 255-272 |
| بيانات النشر: | Springer Science and Business Media LLC, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | 0106 biological sciences, Time Factors, Somatic embryogenesis, Antimetabolites, Meristem, Ascorbic Acid, Plant Science, Biology, hordeum-vulgare l, 01 natural sciences, Antioxidants, molecular characterization, plant-tissue culture, argonaute family, 03 medical and health sciences, chemistry.chemical_compound, Laboratorium voor Plantenveredeling, spruce picea-glauca, Culture Techniques, Gene expression, Genetics, Buthionine sulfoximine, Buthionine Sulfoximine, Oligonucleotide Array Sequence Analysis, 030304 developmental biology, 0303 health sciences, Gene Expression Profiling, Brassica napus, Embryogenesis, brassica-napus, Embryo, Glutathione, somatic embryos, gene-expression, PRI Bioscience, Plant Breeding, chemistry, Biochemistry, microspore embryogenesis, glutathione synthesis, Embryo quality, 010606 plant biology & botany |
| الوصف: | Applications of buthionine sulfoximine (BSO), an inhibitor of GSH (reduced glutathione), which switches the cellular glutathione pool towards the oxidized form GSSG, positively influences embryo quality by improving the structure of the shoot apical meristem and promoting embryo maturation, both of which improve the post-embryonic performance of the embryos. To investigate the mechanisms underlying BSO-mediated improvement in embryo quality the transcript profiles of developing Brassica napus microspore-derived embryos cultured in the absence (control) or presence of BSO were analyzed using a 15,000-element B. napus oligo microarray. BSO applications induced major changes in transcript accumulation patterns, especially during the late phases of embryogenesis. BSO affected the transcription and activities of key enzymes involved in ascorbate metabolism, which resulted in major fluctuations in cellular ascorbate levels. These changes were related to morphological characteristics of the embryos and their post-embryonic performance. BSO applications also activated many genes controlling meristem formation and function, including ZWILLE, SHOOTMERISTEMLESS, and ARGONAUTE 1. Increased expression of these genes may contribute to the improved structural quality of the shoot poles observed in the presence of BSO. Compared to their control counterparts, middle- and late-stage BSO-treated embryos also showed increased accumulation of transcripts associated with the maturation phase of zygotic embryo development, including genes encoding ABA-responsive proteins and storage- and late-embryogenic abundant (LEA) proteins. Overall these transcriptional changes support the observation that the BSO-induced oxidized glutathione redox state allows cultured embryos to reach both morphological and physiological maturity, which in turn guarantees successful regeneration and enhanced post-embryonic growth. |
| وصف الملف: | application/pdf; application/octet-stream |
| تدمد: | 1432-2048 0032-0935 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36a1c3cdfdf1c430d86cb588dbd4a054Test https://doi.org/10.1007/s00425-008-0735-zTest |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....36a1c3cdfdf1c430d86cb588dbd4a054 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322048 00320935 |
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