التفاصيل البيبلوغرافية
| العنوان: |
Neuroprotective potentials of selected natural edible oils using enzyme inhibitory, kinetic and simulation approaches. |
| المؤلفون: |
Mahnashi, Mater H., Alyami, Bandar A., Alqahtani, Yahya S., Alqarni, Ali O., Jan, Muhammad Saeed, Ayaz, Muhammad, Ullah, Farhat, Shahid, Muhammad, Rashid, Umer, Sadiq, Abdul |
| المصدر: |
BMC Complementary Medicine & Therapies; 10/2/2021, Vol. 21 Issue 1, p1-14, 14p, 8 Diagrams, 1 Chart, 2 Graphs |
| مصطلحات موضوعية: |
FUNCTIONAL foods, ACETYLCHOLINESTERASE, GINGER, ALZHEIMER'S disease, ANALYSIS of variance, VEGETABLE oils, CINNAMON, ORGANIC compounds, ANTIOXIDANTS, PHYTOCHEMICALS, NEUROPROTECTIVE agents, DESCRIPTIVE statistics, COMPUTER-assisted molecular modeling, MOLECULAR structure, FREE radical scavengers, ENZYME inhibitors |
| مستخلص: |
Background: Edible oils have proven health benefits in the prevention and treatment of various disorders since the establishment of human era. This study was aimed to appraise neuropharmacological studies on the commonly used edible oils including Cinnamomum verum (CV), Zingiber officinale (ZO) and Cuminum cyminum (CC). Methods: The oils were analyzed via GC-MS for identifications of bioactive compounds. Anti-radicals capacity of the oils were evaluated via 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals scavenging assays. The samples were also tested against two important acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) which are among the important drug targets in Alzheimer's disease. Lineweaver-Burk plots were constructed for enzyme inhibition studies which correspond to velocity of enzymes (Vmax) against the reciprocal of substrate concentration (Km) in the presence of test samples and control drugs following Michaelis-Menten kinetics. Docking studies on AChE target were also carried out using Molecular Operating Environment (MOE 2016.0802) software. Results: (Gas chromatography-mass spectrometry GC-MS) analysis revealed the presence of thirty-four compounds in Cinnamon oil (Cv.Eo), fourteen in ginger oil (Zo.Eo) and fifty-six in cumin oil (Cc.Eo). In the antioxidant assays, Cv.Eo, Zo.Eo and Cc.Eo exhibited IC50 values of 85, 121, 280 μg/ml sequentially against DPPH radicals. Whereas, in ABTS assay, Cv.Eo, Zo.Eo and Cc.Eo showed considerable anti-radicals potentials with IC50 values of 93, 77 and 271 μg/ml respectively. Furthermore, Cv.Eo was highly active against AChE enzyme with IC50 of 21 μg/ml. Zo.Eo and Cc.Eo exhibited considerable inhibitory activities against AChE with IC50 values of 88 and 198 μg/ml respectively. In BChE assay, Cv.Eo, Zo.Eo and Cc.Eo exhibited IC50 values of 106, 101 and 37 μg/ml respectively. Our results revealed that these oils possess considerable antioxidant and cholinesterase inhibitory potentials. As functional foods these oils can be effective remedy for the prevention and management of neurological disorders including AD. Synergistic effect of all the identified compounds was determined via binding energy values computed through docking simulations. Binding orientations showed that all the compounds interact with amino acid residues present in the peripheral anionic site (PAS) and catalytic anionic site (CAS) amino acid residues, oxyanion hole and acyl pocket via π-π stacking interactions and hydrogen bond interactions. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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