نتائج البحث - "Lactate dehydrogenase"

1

Prolong Administration of Methanolic Whole Fruit Extract of Lagenaria breviflora (Benth.) Roberty Provoke Oxidative Stress and Kidney Dysfunction in Male Wistar Rats

المؤلفون: Olubukola Sinbad Olorunnisola, Abiodun Olusoji Owoade, Adewale Adetutu, Ajayi Ayodeji Folorunsho

الوصف: Kidney diseases contributed to the worldwide burden of human diseases, with approximately more than ¾ million deaths annually. The exact reason (s) for the high prevalence of kidney and urinary tract diseases are unknown. The effects of 1 mL of methanolic fruit extract of L. breviflora administered orally, at 0.5 and 1.0 g/kg/body weight once daily for fourteen and twenty-eight days respectively on serum and tissue antioxidants status, electrolytes and the activities of some ‘marker’ enzymes of kidney toxicity in Wister rats was investigated. The control group received 0.9% physiology saline. Using established protocols, the results showed that the extract negatively altered (p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9b0bb3f4531ccc2530a4a4b06db3c43Test
https://zenodo.org/record/8163077Test

2

Fucoxanthin alters the apelin-13/APJ pathway in certain organs of γ-irradiated mice

المؤلفون: Mohamed K. Abdel-Rafei, Noura M Thabet, Nermeen M El Bakary, Ghada El Tawill, Khaled Shaaban Azab, Neama M El Fatih

المصدر: Journal of Radiation Research

مصطلحات موضوعية: Necrosis, Antioxidant, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Fucoxanthin, Xanthophylls, Kidney, Antioxidants, chemistry.chemical_compound, Mice, 0302 clinical medicine, chemistry.chemical_classification, 0303 health sciences, Apelin Receptors, Radiation, Glutathione peroxidase, NF-kappa B, Apelin-13/APJ, Malondialdehyde, Oxidants, Apelin, medicine.anatomical_structure, Liver, Matrix Metalloproteinase 9, Organ Specificity, 030220 oncology & carcinogenesis, Intercellular Signaling Peptides and Proteins, Matrix Metalloproteinase 2, medicine.symptom, MMP-9, Whole-Body Irradiation, Signal Transduction, medicine.medical_specialty, α-7nAchR, 03 medical and health sciences, Lactate dehydrogenase, Internal medicine, medicine, Animals, Radiology, Nuclear Medicine and imaging, Fundamental Radiation Science, 030304 developmental biology, Inflammation, Tissue Inhibitor of Metalloproteinase-1, L-Lactate Dehydrogenase, Monocyte, Glutathione, γ-radiation, Endocrinology, chemistry, Gamma Rays, AcademicSubjects/SCI00960, AcademicSubjects/MED00870

الوصف: Apelin-13 and APJ are implicated in different key physiological processes. This work aims at exploring the radioprotective effect of fucoxanthin (FX) on γ-radiation (RAD)-induced changes in the apelin-13/APJ pathway, which causes damage in the liver, kidney, lung and spleen of mice. Mice were administered FX (10 mg kg–1 day–1, i.p) and exposed to γ-radiation (2.5 Gy week–1) for four consecutive weeks. The treatment of irradiated mice by FX resulted in a significant amendment in protein expression of the apelin-13/APJ/NF-κB signalling pathway concurrently with reduced hypoxia (hypoxia-inducible factor-1α), suppressed oxidative stress marker (malondialdehyde), enhanced antioxidant defence mechanisms (reduced glutathione and glutathione peroxidase), a modulated inflammatory response [interleukin-6 (IL-6), monocyte chemoattractant protein-1, IL-10 and α-7-nicotinic acetylcholine receptor) and ameliorated angiogenic regulators [matrix metalloproteinase (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-1), as well as the tissue damage indicator (lactate dehydrogenase) in organ tissues. In addition, there were significant improvement in serum inflammatory markers tumour necrosis factor-α, IL-10, IL-1β and C-reactive protein compared with irradiated mice. The histopathological investigation of the FX + RAD organ tissues support the biochemical findings where the improvements in the tissues’ architecture were obvious when compared with those of RAD. FX was thus shown to have a noticeable radioprotective action mediated through its regulatory effect on the apelin-13/APJ/NF-κB signalling pathway attributed to its antioxidant and anti-inflammatory activity that was reflected in different physiological processes. It could be recommended to use FX in cases of radiation exposure to protect normal tissues.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e30214c17e6d8f3108e2b41351abd16bTest
http://europepmc.org/articles/PMC8273792Test

3

2, 3-Dimethylsuccinic acid and fulvic acid attenuate lead-induced oxidative misbalance in brain tissues of Nile tilapia Oreochromis niloticus

المؤلفون: Kadry M. Sadek, Yasser S. El-Sayed, Asmaa F. Khafaga, Wesam A. Neamatallah, Ebeed Saleh

المصدر: Environmental Science and Pollution Research. 29:21998-22011

الوصف: Lead has long been known as neurotoxic and immunotoxic heavy metal in human and animals including fish, whereas, 2, 3-dimethylsuccinic acid (DMSA) and fulvic acid (FA) are well-known biological chelators. The present investigation was carried out to assess the potential chelating and antioxidant effects of dietary supplementation with DMSA and FA against lead acetate (Pb)-induced oxidative stress in Nile tilapia, O. niloticus. One-hundred and eighty apparently healthy O. niloticus fish (30 ± 2.5 g) were allocated into six equal groups. The first group was fed on basal diet and served as control, while the second group was fed on DMSA-supplemented basal diets at levels of 30 mg/kg diet; the third group was fed on FA-supplemented basal diet at level of 0.3 mg/kg diet; the forth, fifths, and sixth groups were exposed to 14.4 mg Pb /L water (1/10 LC50) and feed on basal diet only, basal diet supplemented with DMSA (0.3 mg/kg diet), or basal diet supplemented with FA (0.3 mg/kg diet), respectively. Antioxidant and lipid peroxidative status, activity of glucose 6-phosphate dehydrogenase (G6PD), and lactate dehydrogenase (LDH) as well as the histopathologic findings were evaluated in brain tissues, while the Pb residues were evaluated in liver, muscles, and brain tissues. The results of the present study showed that DMSA and FA decreased malondialdehyde (MDA) and Pb residue in tissues of Pb-exposed fish and improved the histologic picture and brain contents of glutathione (GSH), glutathione-s-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), G6PD, LDH, and total antioxidant capacity (TAC). It could be concluded that DMSA and FA supplementation exhibited potential neuroprotective effect against Pb-induced oxidative brain damages in O. niloticus through improvement of antioxidant status of the brain tissue.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::6c56ceab05f4f67e51256bcc746d16b8Test
https://doi.org/10.1007/s11356-021-16359-zTest

4

Selenide Chitosan Sulfate Improved the Hepatocyte Activity, Growth Performance, and Anti-oxidation Capacity by Activating the Thioredoxin Reductase of Chickens In Vitro and In Vivo

المؤلفون: Huiling Qiu, Lele Hou, Shansong Gao, Fu Chen, Lianqin Zhu

المصدر: Biological Trace Element Research. 200:3798-3807

الوصف: Chicken hepatocytes were cultured in vitro and 240 specific pathogen-free (SPF) white leghorns chickens (7 days old) were obtained. The hepatocytes and chickens were randomly allocated to one of six treatment groups: control group; chitosan (COS) group; sodium selenite (Na2SeO3) group; selenide chitosan (COS-Se) group; chitosan sulfate (LS-COS) group; and selenide chitosan sulfate (LS-COS-Se) group. Our results showed that LS-COS-Se increased (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab8ccbd7130b9c8c83aefb19d458ff4eTest
https://doi.org/10.1007/s12011-021-02962-1Test

5

Preparation, structural characterization and neuroprotective effects of polysaccharides from the pericarp of Zanthoxylum bungeanum Maxim against H2O2-induced oxidative damage in PC12 cells

المؤلفون: Qing Su, Yu-Jie Liu, Gang Wang, Zhi-Yang Chen, Lin Chen, Mei-Bian Hu

المصدر: South African Journal of Botany. 142:165-174

مصطلحات موضوعية: Arabinose, chemistry.chemical_classification, Antioxidant, Chromatography, biology, Rhamnose, medicine.medical_treatment, Extraction (chemistry), Plant Science, Malondialdehyde, Polysaccharide, Superoxide dismutase, chemistry.chemical_compound, chemistry, Lactate dehydrogenase, medicine, biology.protein

الوصف: The pericarp of Zanthoxylum bungeanum Maxim (PZM) is one of eight fascinating condiments and also used as a traditional herbal medicine to treat a variety of diseases. In this study, ultrasound-assisted extraction (UAE) was applied for the extraction of polysaccharides from PZM (PPZM) and the extraction conditions were optimized by response surface methodology (RSM). The obtained PPZM yield (2.60 ± 0.06%) was closely agreed with the predicted value (2.58%), and the optimal extraction conditions obtained were: liquid to solid ratio of 32 mL/g, extraction time of 42 min, and extraction temperature of 61 °C. PPZM was determined to be composed of mannose (Man), rhamnose (Rha), galacturonic acid (GalA), glucose (Glu), galactose (Gal), arabinose (Ara) with a molar ratio of 4.93: 5.03: 71.01: 16.01: 24.87: 18.79, and the average molecular weight (Mw) of PPZM was 1.72 × 105 Da. Typical polysaccharide characteristics were determined by fourier transform infrared spectroscopy (FT-IR). PPZM exhibited remarkable antioxidant activities in vitro. More importantly, PPZM effectively protected adrenal phaeochromocytoma PC12 cells against H2O2-induced damage by inhibiting the release of lactate dehydrogenase (LDH) and malondialdehyde (MDA), increasing the level of superoxide dismutase (SOD), and inhibiting abnormal apoptosis. Further experiments indicated that the anti-apoptotic effects of PPZM were closely related to down-regulating the expressions of Bax and Caspase-3, and up-regulating the expression of Bcl-2. Therefore, PPZM can be used as the source of natural antioxidants and neuroprotective agents.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7392dd9007f359bc57cdaffa9254e499Test
https://doi.org/10.1016/j.sajb.2021.06.026Test

6

IN VITRO CYTOTOXIC ASSESSMENT OF CHITOSAN OLIGOSACCHARIDE LACTATE ON HUMAN BLOOD AND LYMPHOCYTE CELLS

المؤلفون: Salim Çeriğ

المصدر: Volume: 10, Issue: 1 79-89
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi-C Yaşam Bilimleri Ve Biyoteknoloji

مصطلحات موضوعية: Antioxidant,chitosan oligosaccharide lactate,cytotoxic,human lymphocyte,lactate dehydrogenase, Human lymphocyte, Antioxidant, Human blood, medicine.medical_treatment, Lymphocyte, Pharmaceutical Science, In vitro, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Biochemistry, CHITOSAN OLIGOSACCHARIDE, Lactate dehydrogenase, medicine, Cytotoxic T cell, Biology, Biyoloji

الوصف: Chitosan oligosaccharides (ChOSs) are the reduced products of chitosan prepared by chemical or enzymatic hydrolysis. The greater solubility and low viscosity of ChOSs are of interest. The present study was the first to evaluate the toxicity of chitosan oligosaccharide lactate (ChOSlac) in human blood. For this purpose, possible oxidative effects of ChOSlac in human whole blood (hWB) and cell viability and membrane integrity effects on lymphocytes (LYMs) were evaluated in the dose range of 10-400 μg/ml and for 24 and 48 hours treatments. Firstly, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were used to measure oxidative damage on hWB serum. Secondly, the cytological effects were evaluated using 2.3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H tetrazolium-5-carboxanilide inner salt (XTT) and lactate dehydrogenase (LDH) assays on LYMs. Exposure of cells to 10-200 μg/ml range doses of ChOSlac caused an increase in antioxidant activity and a decrease in oxidative stress but did not affect cytotoxicity. Conversely, the dose of 400 µg/ml caused a relative increase in oxidative stress and LDH leakage and decreased cell viability. In summary, ChOSlac has been evaluated positively at the specific dose range and exposure times in terms of human health as a contribution to its use in many areas such as being a biocompatible, biodegradable, and drug carrier molecule.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a911fee71323899556bf01fad3fb926Test
https://doi.org/10.18036/estubtdc.798520Test

7

Effect of dietary saffron (Crocus sativus) petal extract on growth performance, blood biochemical indices, antioxidant balance, and immune responses of broiler chickens reared under heat stress conditions

المؤلفون: S. J. Hosseini-Vashan, Ali Hossein Piray

المصدر: Italian Journal of Animal Science, Vol 20, Iss 1, Pp 1338-1347 (2021)

الوصف: To evaluate the alleviative effects of saffron (Crocus sativus) petal extract (SPE) on growth performance, blood biochemical indices, antioxidant status, and immunity of heat-stressed broilers, 250 newly hatched male broiler chickens were randomly allocated to control diet (basal diet, two groups), or control diet supplemented with 300, 500, or 700 mg SPE/kg diet, with five replicates and 10 birds each. The broiler chickens, except one of the control groups, were exposed to heat stress (HS, 37 ± 1 °C, 55% relative humidity) for 6 h/day from day 25 to 42. Heat stress reduced body weight gain (BWG), feed intake (FI), and abdominal fat percentage, and increased feed conversion ratio (FCR, p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a1223079dad65e85c9674e5633f315dTest
https://doi.org/10.1080/1828051x.2021.1921628Test

8

Isolation, characterization and HepG-2 inhibition of a novel proteoglycan from Flammulina velutipes

المؤلفون: Qiuhui Hu, Gaoxing Ma, Anxiang Su, Alfred Mugambi Mariga, Wenjian Yang, Hui Xu, Jianhui Liu, Fei Pei, Minhao Xie, Hu Ye

المصدر: International Journal of Biological Macromolecules. 189:11-17

الوصف: Flammulina velutipes has anti-inflammatory, immunomodulatory, antioxidant and many bioactive properties with high contents of carbohydrate, proteins and fibers. In this study, a novel proteoglycan with polysaccharide complexes and protein chain, named PGD1-1, was isolated from F. velutipes. The structural characteristics of PGD1-1 were then determined, and its anti-proliferation and pro-apoptotic activities against HepG-2 cells were demonstrated in vitro. Results proved that the average molecular weight of PGD1-1 was 32.71 kDa, and the carbohydrate and protein contents were 93.35 and 2.33%, respectively. The protein moiety was bonded to a polysaccharide chain via O-glycosidic linkage. The monosaccharides consisted of d -glucose, D-galactose and D-xylose in a molar ratio of 21.90:2.84:1.00. PGD1-1 significantly inhibited the proliferation of HepG-2 cells by affecting cell lipid peroxidation and nitric oxide production. In addition, PGD1-1 promoted the apoptosis of HepG-2 cells, especially the early apoptosis. These findings proved that PGD1-1 was a novel potent ingredient against the proliferation of HepG-2, which will provide a theoretical basis for the development and utilization of the functional ingredients of the F. velutipes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c860ab0029afbaae27da19b4fd769338Test
https://doi.org/10.1016/j.ijbiomac.2021.08.086Test

9

Experimental model of acute urate oxalate nephrolithiasis in Wistar rats

المصدر: Zhurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia». :98-106

الوصف: Введение. В последнее время возросла частота регистрации случаев уратно-оксалатного нефролитиаза. Для разработки новых методов фармакологической коррекции и оценки эффективности фармакотерапии необходимо создание адекватной модели заболевания. Цель исследования - моделирование смешанного уратно-оксалатного нефролитиаза на крысах Wistar и изучение биохимической и гистологической картины патологии. Методика. Эксперимент выполнен на 30 самцах крыс Wistar, разделенных на группу сравнения (n=10) и экспериментальную группу (n=20). Животным экспериментальной группы ежедневно на протяжении 7 сут внутрижелудочно через зонд вводили смесь оксониевой и мочевой кислот в дозах 500 и 1000 мг/кг соответственно и предоставляли в качестве питья 1% раствор этиленгликоля. В моче крыс обеих групп измеряли активность лактатдегидрогеназы и γ-глутамилтрансферазы, в гомогенате почек - концентрацию тиобарбитуратреактивных продуктов, общую прооксидантную и антиоксидантную активность, активность глутатионпероксидазы, каталазы, супероксиддисмутазы. Наличие конкрементов в почках и наличие воспалительной реакции оценивали по результатам морфологического исследования. Результаты. В моче крыс экспериментальной группы к 7-м сут эксперимента активность лактатдегидрогеназы возросла более чем в 20 раз относительно исходного уровня, в группе сравнения не происходило статистически значимых изменений активности лактатдегидрогеназы по сравнению с исходным уровнем. Концентрация тиобарбитуратреактивных продуктов и активность каталазы в гомогенате почек крыс экспериментальной группы на 7-е сут исследования были в 1,3 и 1,6 раза соответственно выше аналогичных показателей группы сравнения, а общая прооксидантная и общая антиоксидантная активность, а также активность супероксиддисмутазы в гомогенате почек крыс подопытной группы статистически значимо снизились в 3,1, 1,8 и 3,2 раза соответственно относительно уровня группы сравнения. В группе сравнения почечные конкременты отсутствовали, а в экспериментальной были выявлены в 100% случаев в количестве 20,2±2,35 со средней площадью 540,55±47,7 мкм2 с признаками сопутствующих воспалительных и дистрофических процессов. Заключение. При недельном применении 1% раствора этиленгликоля и смеси оксониевой и мочевой кислот в дозах 500 и 1000 мг/кг соответственно формируется уратно-оксалатный нефролитиаз, характеризующийся повышением активности лактатдегидрогеназы в моче, активацией окислительных процессов и образованием смешанных депозитов с наличием выраженной воспалительной инфильтрации, кистозного расширения почечных канальцев, а также дистрофических изменений канальцевого эпителия. Introduction. At present, the incidence of urate-oxalate nephrolithiasis has increased. For the development of new methods of pharmacological treatment of this disease, it is necessary to create an adequate model that makes it possible to assess the effectiveness of pharmacotherapy. The aim - create a model of mixed urate-oxalate nephrolithiasis in Wistar rats and to study the biochemical and histological picture of the pathology. Methods. Experiments were carried out on 30 male Wistar rats, divided into a comparison group (n = 10) and an experimental group (n = 20). For formation of urate-oxalate nephrolithiasis, the experimental group was injected daily for 7 days intragastrically a mixture of oxonic and uric acids at doses of 500 and 1000 mg/kg, respectively, and these rats were also provided a drinking 1% solution of ethylene glycol. The activities of lactate dehydrogenase and γ-glutamyl transferase were measured in the urine of both groups. In the kidney homogenate, the concentration of thiobarbiturate-reactive products, total pro-oxidant and antioxidant activity, and activities of glutathione peroxidase, catalase, and superoxide dismutase were measured. Severity of lithogenesis, and inflammation was assessed based on the results of morphological studies. Results. In the urine of rats of the experimental group, the activity of lactate dehydrogenase increased more than 20 times relative to the initial level. In the comparison group, there were no significant biochemical changes. The concentration of thiobarbiturate-reactive substances and the activity of catalase in the kidney homogenate experimental group on the 7th day the were 1.3 and 1.6 times higher, respectively, than those of the comparison group. The total pro-oxidant and total antioxidant activity, as well as the activity of superoxide dismutase, decreased significantly by 3.1, 1.8 and 3.2 times, respectively. In the comparison group, renal calculi were absent, but in the experimental group, they were found in 100% of cases in the number of 20.2 ± 2.4 with an average area of 140.55 ± 47.7 μm2, accompanied by inflammatory and degenerative processes. Conclusion. With a weekly application of a 1% solution of ethylene glycol and a mixture of oxonic and uric acids at doses of 500 and 1000 mg/kg, respectively, a pronounced urate-oxalate nephrolithiasis is formed. This was characterized by an increase in the activity of lactate dehydrogenase in the urine, activation of oxidative processes, and the formation of mixed deposits associated with pronounced inflammatory infiltration, cystic dilatation of the renal tubules, as well as dystrophic changes in the tubular epithelium.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::13e7bb056f5b5ccb40639abc760f1b82Test
https://doi.org/10.25557/0031-2991.2021.03.98-106Test

10

Freeze-Dried Powder of Rubus coreanus Miquel Ameliorates Isoproterenol-Induced Oxidative Stress and Tissue Damage in Rats

المؤلفون: Jin Tae Kim, Ho Jin Park, Ji Hyun Moon, Shuai Qiu, Seung Beom Lee, Hong Jin Lee, Yimeng Zhou

المصدر: Journal of Microbiology and Biotechnology. 31:1256-1261

الوصف: Rubus coreanus Miquel (bokbunja), Korean black raspberry, is known to possess various phytochemicals that exert antioxidative, anti-inflammatory, and anti-cancer effects. However, most studies on Rubus coreanus Miquel have been performed with the solvent extracts and/or a single component to demonstrate the efficacy, while studies evaluating the effect of the whole fructus of Rubus coreanus Miquel are limited. In this study, therefore, we employed the isoproterenol (IPN)-induced myocardial infarction model and investigated the effect of freeze-dried powder of Rubus coreanus Miquel (RCP) on oxidative stress and prevention of organ damage. Oral administration of RCP reduced the level of toxicity markers, alanine transaminase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) without affecting body weight and diet intake. The oxidative stress marker glutathione (GSH) increased about 45% and malonaldehyde (MDA) decreased about 27% compared to the IPN group with RCP-H (3%) administration. By histological analysis, IPN induced significant myocardial damage in the heart and vascular injury in the liver, and RCP administration ameliorated the damages in a dose-dependent manner. Taken together, RCP activated the antioxidant system leading to prevention of damage to organs by IPN in rats, making it possible to expect beneficial efficacies by consuming the whole fructus of Rubus coreanus Miquel.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::38658064462046ee4fb029b38ead8161Test
https://doi.org/10.4014/jmb.2106.06047Test

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