Potentiation of curing by a broad-host-range self-transmissible vector for displacing resistance plasmids to tackle AMR
| العنوان: | Potentiation of curing by a broad-host-range self-transmissible vector for displacing resistance plasmids to tackle AMR |
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| المؤلفون: | Anand Prakash Maurya, Georgina S. Lloyd, Anthony S. Haines, Alessandro Lazdins, Claire E Miller, Jan-Ulrich Kreft, Shuting Liu, Melissa Chamberlain, M. Kamruzzaman, Elton R. Stephens, Jonathan R. Iredell, Mona Haratianfar, Christopher M. Thomas, Mark A. Webber |
| المصدر: | PLoS ONE, Vol 15, Iss 1, p e0225202 (2020) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Sucrose, Molecular biology, Artificial Gene Amplification and Extension, Disaccharides, Biochemistry, Polymerase Chain Reaction, Mice, Plasmid, Antibiotics, Mobile Genetic Elements, Medicine and Health Sciences, Vector (molecular biology), Genetics, 0303 health sciences, education.field_of_study, Multidisciplinary, Antimicrobials, Organic Compounds, Escherichia coli Proteins, Drugs, Bacterial Infections, Genomics, Nucleic acids, Chemistry, Tetracyclines, Conjugation, Genetic, Physical Sciences, Medicine, Plasmids, Research Article, DNA Copy Number Variations, Forms of DNA, Science, Population, Carbohydrates, Context (language use), DNA Primase, Biology, DNA construction, Microbiology, Host Specificity, Bacterial genetics, 03 medical and health sciences, Genetic Elements, Microbial Control, Drug Resistance, Bacterial, DNA-binding proteins, Escherichia coli, Animals, Humans, education, Gene, 030304 developmental biology, Pharmacology, Biology and life sciences, 030306 microbiology, Organic Chemistry, Wild type, Chemical Compounds, Proteins, DNA, Gastrointestinal Microbiome, Research and analysis methods, Disease Models, Animal, Molecular biology techniques, Antibiotic Resistance, Plasmid Construction, Antimicrobial Resistance, Low copy number |
| الوصف: | Plasmids are potent vehicles for spread of antibiotic resistance genes in bacterial populations and often persist in the absence of selection due to efficient maintenance mechanisms. We previously constructed non-conjugative high copy number plasmid vectors that efficiently displace stable plasmids from enteric bacteria in a laboratory context by blocking their replication and neutralising their addiction systems. Here we assess a low copy number broad-host-range self-transmissible IncP-1 plasmid as a vector for such curing cassettes to displace IncF and IncK plasmids. The wild type plasmid carrying the curing cassette displaces target plasmids poorly but derivatives with deletions near the IncP-1 replication origin that elevate copy number about two-fold are efficient. Verification of this in mini IncP-1 plasmids showed that elevated copy number was not sufficient and that the parB gene, korB, that is central to its partitioning and gene control system, also needs to be included. The resulting vector can displace target plasmids from a laboratory population without selection and demonstrated activity in a mouse model although spread is less efficient and requires additional selection pressure. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caf20606f2cc14446cd3958b4e8a1ff5Test https://doaj.org/article/4261456de09342e2af36055c3574d1ffTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....caf20606f2cc14446cd3958b4e8a1ff5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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