التفاصيل البيبلوغرافية
| العنوان: |
Further investigation of the epitope recognized by the new monoclonal antibody 2C9. |
| المؤلفون: |
KATO, KEITARO, YOSHIKAWA, KAZUHIRO, TAKI, TOMOHIRO, SHITARA, KENYA, NAKAMURA, KAZUYASU, HIROTA, MAIKO, HANAI, NOBUO, NAKAMURA, KOGENTA, KOKUBO, HIROTO, MITSUI, KENJI, YAMADA, YOSHIAKI, HONDA, NOBUAKI, UEDA, RYUZO, SAGA, SHINSUKE, FUKATSU, HIDETOSHI |
| المصدر: |
International Journal of Urology; Aug2003, Vol. 10 Issue 8, p439-444, 6p |
| مصطلحات موضوعية: |
MONOCLONAL antibodies, ANTIGENS, AMINO acid sequence |
| مستخلص: |
Abstract Objective: We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody. Methods: The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system. Results: The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA. Conclusion: The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
