Phosphotyrosine phosphatase inhibitor bisperoxovanadium endows myogenic cells with enhanced muscle stem cell functions via epigenetic modulation of Sca-1 and Pw1 promoters
| العنوان: | Phosphotyrosine phosphatase inhibitor bisperoxovanadium endows myogenic cells with enhanced muscle stem cell functions via epigenetic modulation of Sca-1 and Pw1 promoters |
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| المؤلفون: | Frédéric Relaix, Marina Bouché, Francesco Fazi, Silvia Masciarelli, Giovanna Marazzi, Valeria Marrocco, Ilaria Iosue, Luca Madaro, Sonia Alonso-Martin, Piera Smeriglio, David Sassoon |
| المساهمون: | Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), French Ministry of Research ``Chaired Excellence', Muscular Dystrophy Association of America, Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University of Rome, Duchenne Parent Project (Amsterdam, The Netherlands), European Community [Health-F5-2009-223098], ENDOSTEM (Activation of Vasculature Associated Stem Cells for the Repair and Maintenance of Muscle Tissue) [241440], Agence Nationale de la Recherche (Laboratoire d'Excellence Revive, Investissement d'Avenir) [ANR-10-LABX-73], IHU-ICAN projects, Italian Ministry for University and Research, Sapienza University of Rome, French Association against Myopathies (AFM), Telethon Italy, Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU) |
| المصدر: | FASEB Journal FASEB Journal, Federation of American Society of Experimental Biology, 2016, 30 (4), pp.1404-1415. ⟨10.1096/fj.15-275420⟩ FASEB Journal, 2016, 30 (4), pp.1404-1415. ⟨10.1096/fj.15-275420⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Nude, Gene Expression, muscle cell fate, Biochemistry, Transgenic, Epigenesis, Genetic, Myoblasts, Mice, Myocyte, Antigens, Ly, Promoter Regions, Genetic, Cells, Cultured, Microscopy, muscle regeneration, Cultured, Blotting, Reverse Transcriptase Polymerase Chain Reaction, Skeletal, 3. Good health, Cell biology, Chromatin, Muscle, Settore BIO/17 - ISTOLOGIA, Stem cell, Western, Biotechnology, Vanadium Compounds, cell reprogramming, muscle stem cells, PW1 interstitial cells, cell stemness, Cells, Myoblasts, Skeletal, Blotting, Western, Kruppel-Like Transcription Factors, Mice, Nude, Mice, Transgenic, Biology, Cell fate determination, Fluorescence, Interstitial cell, Cell Line, Promoter Regions, 03 medical and health sciences, Genetic, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, Animals, Regeneration, Antigens, Progenitor cell, Muscle, Skeletal, Cell reprogramming, Cell stemness, Muscle cell fate, Muscle regeneration, Membrane Proteins, Microscopy, Fluorescence, Muscle Cells, Protein Tyrosine Phosphatases, Molecular Biology, Transplantation, 030104 developmental biology, Ly, Cell culture, Epigenesis |
| الوصف: | International audience; Understanding the regulation of the stem cell fate is fundamental for designing novel regenerative medicine strategies. Previous studies have suggested that pharmacological treatments with small molecules provide a robust and reversible regulation of the stem cell program. Previously, we showed that treatment with a vanadium compound influences muscle cell fate in vitro. In this study, we demonstrate that treatment with the phosphotyrosine phosphatase inhibitor bisperoxovanadium (BpV) drives primary muscle cells to a poised stem cell stage, with enhanced function in muscle regeneration in vivo following transplantation into injured muscles. Importantly, BpV-treated cells displayed increased self-renewal potential in vivo and replenished the niche in both satellite and interstitial cell compartments. Moreover, we found that BpV treatment induces specific activating chromatin modifications at the promoter regions of genes associated with stem cell fate, including Sca-1 and Pw1. Thus, our findings indicate that BpV resets the cell fate program by specific epigenetic regulations, such that the committed myogenic cell fate is redirected to an earlier progenitor cell fate stage, which leads to an enhanced regenerative stem cell potential.-Smeriglio, P., Alonso-Martin, S., Masciarelli, S., Madaro, L., Iosue, I., Marrocco, V., Relaix, F., Fazi, F., Marazzi, G., Sassoon, D. A., Bouche, M. Phosphotyrosine phosphatase inhibitor bisperoxovanadium endows myogenic cells with enhanced muscle stem cell functions via epigenetic modulation of Sca-1 and Pw1 promoters. FASEB J. 30, 1404-1415 (2016). www.fasebj.org |
| اللغة: | English |
| تدمد: | 0892-6638 1530-6860 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::919f708e5011c2c3f5c984ebf0f47f4bTest https://hal.archives-ouvertes.fr/hal-01515295Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....919f708e5011c2c3f5c984ebf0f47f4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 08926638 15306860 |
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