دورية أكاديمية
BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology
| العنوان: | BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
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| المؤلفون: | Eirin, Maria Emilia, Carignano, Hugo Adrian, Shimizu, Ernesto, Pando, Maria de los Angeles, Zumarraga, Martin Jose, Magnano, Gabriel, Macías, Analía, Garbaccio, Sergio Gabriel, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Ferrara Muñiz, Ximena, Cataldi, Angel Adrian, Paolicchi, Fernando, Poli, Mario Andres |
| المصدر: | Research in Veterinary Science 130 : 118-125 (June 2020) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
| مصطلحات موضوعية: | Ganado Bovino, Enfermedades de los Animales, Polimorfismo, Tuberculosis, Mycobacterium bovis, Cattle, Animal Diseases, Polymorphism, Mycobacterium bovis Infections, Antigens, Infeccion por Mycobacterium bovis, Antígenos, Tuberculosis Bovina, Bovine Leucocyte Antigen |
| الوصف: | Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or susceptibility to bovine diseases. We evaluated the association between BoLA DRB3.2 polymorphisms and bTB pathology scores in dairy and beef cattle breeds of Argentina. Most bovines exhibited visible lesions compatible with tuberculosis and, furthermore, 150 (85.7%) were also positive by bacteriology. A pathology index showed a variable degree of disease, from 3 to 76 (median pathology score = 9 (IQR: 7–15)). Thirty-five BoLA DRB3.2 alleles were identified with an associated frequency from 16% to 0.3%, distributed 73% (n = 128) in heterozygosis and 27% (n = 47) in homozygosis, with 12 BoLA DRB3.2 alleles (*0101, *1101, *1501, *0201, *2707 *1001, *1002, *1201, *14011, *0501 *0902 and *0701) representing the 74.7% of the population variability. A functional analysis grouped them in 4 out of 5 clusters (A-D), suggesting a functional overlapping. Among the 90 identified genotypes, *1101/*1101, *1101/*1501 and *0101/*0101 were the most frequent (10%, 8.9% and 8.9%, respectively). No association was detected between the pathology scores and a specific DRB3.2 allele (p > .05). Animals infected with M. bovis spoligotype SB0153 showed a significantly higher pathology score than those affected by the spoligotype SB0145 (p = .018). Furthermore, the Aberdeen Angus breed exhibited highest pathological scores (p < .0001), which were associated with disseminated lesion, thus suggesting that the host component could be important to the disease progression. ; Instituto de Biotecnología ; Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Carignano, Hugo Adrian. Instituto Nacional de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0034-5288 |
| العلاقة: | http://hdl.handle.net/20.500.12123/7706Test; https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test; https://doi.org/10.1016/j.rvsc.2020.03.001Test |
| DOI: | 10.1016/j.rvsc.2020.03.001 |
| الإتاحة: | https://doi.org/20.500.12123/7706Test https://doi.org/10.1016/j.rvsc.2020.03.001Test https://hdl.handle.net/20.500.12123/7706Test https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.B3112A18 |
| قاعدة البيانات: | BASE |
| تدمد: | 00345288 |
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| DOI: | 10.1016/j.rvsc.2020.03.001 |