التفاصيل البيبلوغرافية
| العنوان: |
Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC. |
| المؤلفون: |
Benavente, Maria C. Rodriguez, Hakeem, Zainab A., Davis, Alexander R., Murray, Nathan B., Azadi, Parastoo, Mace, Emily M., Barb, Adam W. |
| المصدر: |
Scientific Reports; 4/4/2024, Vol. 14 Issue 1, p1-14, 14p |
| مصطلحات موضوعية: |
KILLER cells, ANTIBODY-dependent cell cytotoxicity, FLOW cytometry, PROCESS capability, MANNOSIDASES |
| مستخلص: |
Natural killer (NK) cells destroy tissue that have been opsonized with antibodies. Strategies to generate or identify cells with increased potency are expected to enhance NK cell-based immunotherapies. We previously generated NK cells with increased antibody-dependent cell mediated cytotoxicity (ADCC) following treatment with kifunensine, an inhibitor targeting mannosidases early in the N-glycan processing pathway. Kifunensine treatment also increased the antibody-binding affinity of Fc γ receptor IIIa/CD16a. Here we demonstrate that inhibiting NK cell N-glycan processing increased ADCC. We reduced N-glycan processing with the CRIPSR-CAS9 knockdown of MGAT1, another early-stage N-glycan processing enzyme, and showed that these cells likewise increased antibody binding affinity and ADCC. These experiments led to the observation that NK cells with diminished N-glycan processing capability also revealed a clear phenotype in flow cytometry experiments using the B73.1 and 3G8 antibodies binding two distinct CD16a epitopes. We evaluated this "affinity profiling" approach using primary NK cells and identified a distinct shift and differentiated populations by flow cytometry that correlated with increased ADCC. [ABSTRACT FROM AUTHOR] |
|
Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder