Interleukin-10 Inhibits theIn vitroProliferation of Human Activated Leukemic CD5+B-Cells
| العنوان: | Interleukin-10 Inhibits theIn vitroProliferation of Human Activated Leukemic CD5+B-Cells |
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| المؤلفون: | Kathryn M. Weston, Robert L. Raison, Stuart G. Tangye |
| المصدر: | Leukemia & Lymphoma. 31:121-130 |
| بيانات النشر: | Informa UK Limited, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Cancer Research, Cell Survival, Chronic lymphocytic leukemia, Immunoglobulins, CD5 Antigens, Lymphocyte Activation, Tumor Cells, Cultured, medicine, Humans, Secretion, RNA, Neoplasm, biology, Interleukin, Hematology, medicine.disease, Leukemia, Lymphocytic, Chronic, B-Cell, Molecular biology, In vitro, Interleukin-10, Interleukin 10, Oncology, Apoptosis, biology.protein, Cytokines, Tetradecanoylphorbol Acetate, Antibody, CD5, Cell Division |
| الوصف: | B-cell chronic lymphocytic leukemia (B-CLL) is characterised by the proliferation and accumulation of sIgM+/CD5+ B-cells that fail to progress to the final stages of B-cell development. Despite their developmental arrest, leukemic CD5+ B-cells can undergo proliferation in vitro in the presence of different activators including phorbol esters, antibodies to cell surface antigens and human cytokines. Interleukin-10 (IL-10) has recently been found to inhibit CLL B-cell function in vitro by inducing apoptosis and down-regulating expression of bcl-2. Here, we examined the effect of IL-10 on proliferation, RNA synthesis, immunoglobulin (IgM) secretion and viability of leukemic CD5+ B-cells induced by activation with the phorbol ester PMA, alone or in combination with anti-Ig. IL-10 reduced PMA and PMA/anti-Ig induced proliferation and RNA synthesis by 50-80% and 15-40% respectively. Although proliferation and RNA synthesis induced by PMA/anti-Ig could be enhanced by the addition of IL-2, IL-4, IL-13, IFN-gamma or TNF-alpha, the presence of these cytokines failed to abrogate the IL-10-mediated inhibition of leukemic CD5+ B-cell activation. In contrast to the effects on proliferation and RNA synthesis, IL-10 did not inhibit IgM secretion, and had only a minimal effect on the viability of activated cells. Our results indicate that IL-10 inhibits proliferation of leukemic CD5+ B-cells by a mechanism distinct from induction of apoptosis and support the proposal for the utilisation of IL-10 in the therapy of B-CLL. |
| تدمد: | 1029-2403 1042-8194 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70abe3aee6374cf2bdaca1ccfa01db8cTest https://doi.org/10.3109/10428199809057592Test |
| رقم الانضمام: | edsair.doi.dedup.....70abe3aee6374cf2bdaca1ccfa01db8c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10292403 10428194 |
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