التفاصيل البيبلوغرافية
| العنوان: |
Antibacterial activity of the CAP18 peptide against Xanthomonas citri ssp. citri, the causative agent of citrus canker, as evaluated by in vitro and in silico studies. |
| المؤلفون: |
Habibollahi, Faezeh1 (AUTHOR), Hosseinipour, Akbar2 (AUTHOR) hosseini@uk.ac.ir, Lohrasbi‐Nejad, Azadeh1 (AUTHOR) a.lohrasbi@uk.ac.ir |
| المصدر: |
Annals of Applied Biology. Jul2022, Vol. 181 Issue 1, p93-106. 14p. |
| مصطلحات موضوعية: |
*CITRUS canker, *XANTHOMONAS campestris, *CITRUS greening disease, *BILAYER lipid membranes, *MOLECULAR dynamics, *ANTIBACTERIAL agents, *MEMBRANE lipids |
| مستخلص: |
The bacterium Xanthomonas citri ssp. citri (Xcc), causing citrus canker disease, poses a severe threat to citrus production worldwide. New approaches for managing the disease are required because of the limited number of bactericides available, reported pathogen resistance to copper pesticides, and the side effects of such compounds on citrus trees and the environment. In this study, the bacterium was isolated from a Mexican lime tree showing typical disease symptoms and identified as Xcc using conventional biochemical tests and specific PCR. The antibacterial activity of the recombinant peptide CAP18 (rCAP18) against the Xcc bacterium was assessed. Measuring the MIC parameter showed that purified rCAP18 caused bacterial growth inhibition by 90% at a concentration of 4.5 μM. Furthermore, a heterogeneous membrane system consisting of POPE:POPG:TOCL (5:1:3) was created using molecular dynamics simulation. The interaction effect of the oligomeric structure of rCAP18 (rCAP18OLIGO) on the components of a lipid bilayer membrane was determined by measuring the membrane thickness, the order parameter of the acyl chains and the area of each phospholipid. The results obtained from simulation and measurement of order parameters showed that, among the three phospholipids, rCAP18OLIGO more affected the orientation of carbon atoms in POPG. Fluctuations of average area per lipid for each phospholipid showed that rCAP18OLIGO had more interactions with POPG > TOCL > POPE, respectively, in the outer half of the membrane. The results obtained from in silico studies confirmed that the penetration of rCAP18OLIGO into the lipid bilayer membrane reduced the membrane thickness and caused its deformation during the simulation time. This mechanism is possibly the leading factor of Xcc growth inhibition by rCAP18 in vitro. Therefore, the rCAP18 peptide as an antibacterial agent can provide a way to develop a new approach to control the citrus canker disease. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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