دورية أكاديمية
Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract
| العنوان: | Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract |
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| المؤلفون: | Shaikh, Haroon, Vargas, Juan Gamboa, Mokhtari, Zeinab, Jarick, Katja J., Ulbrich, Maria, Mosca, Josefina Peña, Viera, Estibaliz Arellano, Graf, Caroline, Le, Duc-Dung, Heinze, Katrin G., Büttner-Herold, Maike, Rosenwald, Andreas, Pezoldt, Joern, Huehn, Jochen, Beilhack, Andreas |
| المصدر: | Frontiers in immunology, 12 |
| سنة النشر: | 2021 |
| المجموعة: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| مصطلحات موضوعية: | Adoptive Transfer [MeSH], Mesentery [MeSH], Transplantation Tolerance [MeSH], Mice, Inbred C57BL [MeSH], T-Lymphocytes/metabolism [MeSH], Histocompatibility Antigens Class II/immunology [MeSH], acute graft-versus host disease, Cell Differentiation [MeSH], Lymph Nodes/metabolism [MeSH], Lymphocyte Activation [MeSH], Acute Disease [MeSH], Lymph Nodes/transplantation [MeSH], mesenteric lymph node, Phenotype [MeSH], Disease Models, Animal [MeSH], lymph node transplantation, mouse models, alloreactive T cells, Immunology, Cell Proliferation [MeSH], T-Lymphocytes/immunology [MeSH], Hematopoietic Stem Cell Transplantation [MeSH], Animals [MeSH], Graft vs Host Disease/metabolism [MeSH], Graft Survival [MeSH], Lymph Nodes/immunology [MeSH], Mice [MeSH], Graft vs Host Disease/immunology [MeSH] |
| الوصف: | Mesenteric lymph nodes (mLNs) are sentinel sites of enteral immunosurveillance and immune homeostasis. Immune cells from the gastrointestinal tract (GIT) are constantly recruited to the mLNs in steady-state and under inflammatory conditions resulting in the induction of tolerance and immune cells activation, respectively. Surgical dissection and transplantation of lymph nodes (LN) is a technique that has supported seminal work to study LN function and is useful to investigate resident stromal and endothelial cell biology and their cellular interactions in experimental disease models. Here, we provide a detailed protocol of syngeneic mLN transplantation and report assays to analyze effective mLN engraftment in congenic recipients. Transplanted mLNs allow to study T cell activation and proliferation in preclinical mouse models. Donor mLNs proved viable and functional after surgical transplantation and regenerated blood and lymphatic vessels. Immune cells from the host completely colonized the transplanted mLNs within 7-8 weeks after the surgical intervention. After allogeneic hematopoietic cell transplantation (allo-HCT), adoptively transferred allogeneic CD4 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://repository.publisso.de/resource/frl:6474264Test; https://doi.org/10.3389/fimmu.2021.689896Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352558Test/ |
| DOI: | 10.3389/fimmu.2021.689896 |
| الإتاحة: | https://doi.org/10.3389/fimmu.2021.689896Test https://repository.publisso.de/resource/frl:6474264Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352558Test/ |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.D4301957 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2021.689896 |
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