Selective Requirement for CD40-CD154 in Drug-Induced Type 1 Versus Type 2 Responses to Trinitrophenyl-Ovalbumin
| العنوان: | Selective Requirement for CD40-CD154 in Drug-Induced Type 1 Versus Type 2 Responses to Trinitrophenyl-Ovalbumin |
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| المؤلفون: | Louis Boon, Raymond Pieters, Pauline van Helden, Seema Ramdien-Murli, Rob Bleumink, M. Bol, Stefan Nierkens, Peter J.S. van Kooten |
| المصدر: | The Journal of Immunology. 168:3747-3754 |
| بيانات النشر: | The American Association of Immunologists, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | medicine.medical_specialty, Ovalbumin, Injections, Subcutaneous, T cell, CD40 Ligand, Immunology, Dose-Response Relationship, Immunologic, CD11c, Immunoglobulin E, Streptozocin, Mice, Th2 Cells, Immune system, Picrates, Antigens, CD, Internal medicine, medicine, Animals, Immunology and Allergy, CD40 Antigens, CD154, CD86, Mice, Inbred BALB C, Membrane Glycoproteins, CD40, biology, Immune Sera, Penicillamine, hemic and immune systems, Th1 Cells, Intercellular Adhesion Molecule-1, Molecular biology, medicine.anatomical_structure, Endocrinology, Phenytoin, B7-1 Antigen, biology.protein, Female, B7-2 Antigen, Haptens, CD80 |
| الوصف: | CD154 is transiently expressed by activated T cells and interacts with CD40 on B cells, dendritic cells, macrophages, and monocytes. This costimulatory receptor-ligand couple seems decisive in Ag-driven immune responses but may be differentially involved in type 1 vs type 2 responses. We studied the importance of CD40-CD154 in both responses using the reporter Ag popliteal lymph node assay in which selectively acting drugs generate clearly polarized type 1 (streptozotocin) or type 2 (D-penicillamine, diphenylhydantoin) responses to a constant coinjected Ag in the same mouse strain. Treatment of mice with anti-CD154 reduced characteristic immunological parameters in type 2 responses (B and CD4+ T cell proliferation, IgG1 and IgE Abs, and IL-4 secretion) and only slightly affected the type 1 response (small decrease in IFN-γ production, influx of CD11c+ and F4/80+ cells, and prevention of architectural disruption of the lymph node, but no effect on IgG2a Ab and TNF-α secretion or B and CD4+ T cell proliferation). The findings indicate that the CD40-CD154 costimulatory interaction is a prerequisite in drug-induced type 2 responses and is only marginally involved in type 1 responses. The observed expression patterns of CD80 and CD86 on different APC (B cells in type 2 and dendritic cells in type 1) may be responsible for this discrepancy. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2752be0e06fd88efad3a0ecd05d256d8Test https://doi.org/10.4049/jimmunol.168.8.3747Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2752be0e06fd88efad3a0ecd05d256d8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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