نتائج البحث - "Nam-In Baek"

1

Pharmacological inhibition of androgen receptor expression induces cell death in prostate cancer cells

المؤلفون: Jueun Kim, Kyoung-Hwa Seo, In-Sung Song, Choung-Soo Kim, Sung-Wuk Jang, Nam-In Baek, Yunlim Kim, Yu Jeong Jeong

المصدر: Cellular and Molecular Life Sciences. 77:4663-4673

مصطلحات موضوعية: Male, Programmed cell death, bcl-X Protein, Down-Regulation, Mice, Nude, Antineoplastic Agents, Bcl-xL, urologic and male genital diseases, TMPRSS2, Mice, 03 medical and health sciences, Cellular and Molecular Neuroscience, Downregulation and upregulation, Cell Line, Tumor, LNCaP, Animals, Humans, Molecular Biology, Cell Proliferation, Membrane Potential, Mitochondrial, Pharmacology, 0303 health sciences, Cell Death, biology, Chemistry, 030302 biochemistry & molecular biology, Prostate, Prostatic Neoplasms, Cell Biology, Prostate-Specific Antigen, Saponins, Gene Expression Regulation, Neoplastic, Androgen receptor, Disease Models, Animal, Cytosol, Receptors, Androgen, Cell culture, PC-3 Cells, Androgens, biology.protein, Cancer research, Molecular Medicine, Female, Reactive Oxygen Species

الوصف: The androgen receptor (AR) plays an important role in the pathogenesis and development of prostate cancer (PCa). Mostly, PCa progresses to androgen-independent PCa, which has activated AR signaling from androgen-dependent PCa. Thus, inhibition of AR signaling may be an important therapeutic target in androgen-dependent and castration-resistant PCa. In this study, we determined the anticancer effect of a newly found natural compound, sakurasosaponin (S-saponin), using androgen-dependent and castration-resistant PCa cell lines. S-saponin induces mitochondrial-mediated cell death in both androgen-dependent (LNCaP) and castration-resistant (22Rv1 and C4-2) PCa cells, via AR expression. S-saponin treatment induces a decrease in AR expression in a time- and dose-dependent manner and a potent decrease in the expression of its target genes, including prostate-specific antigen (PSA), transmembrane protease, serin 2 (TMPRSS2), and NK3 homeobox 1 (NKX3.1). Furthermore, S-saponin treatment decreases B-cell lymphoma-extra large (Bcl-xL) and mitochondrial membrane potential, thereby increasing the release of cytochrome c into the cytosol. Moreover, Bcl-xL inhibition and subsequent mitochondria-mediated cell death caused by S-saponin were reversed by Bcl-xL or AR overexpression. Interestingly, S-saponin-mediated cell death was significantly reduced by a reactive oxygen species (ROS) scavenger, N-acetylcystein. Animal xenograft experiments showed that S-saponin treatment significantly reduced tumor growth of AR-positive 22Rv1 xenografts but not AR-negative PC-3 xenografts. Taken together, for the first time, our results revealed that S-saponin induces mitochondrial-mediated cell death in androgen-dependent and castration-resistant cells through regulation of AR mechanisms, including downregulation of Bcl-xL expression and induction of ROS stress by decreasing mitochondrial membrane potential.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f83f170931aa2cc855893ec6ddc09d4cTest
https://doi.org/10.1007/s00018-019-03429-2Test

2

Three New Phthalide Glycosides from the Rhizomes of

المؤلفون: Hyoung-Geun, Kim, Seon Min, Oh, Na Woo, Kim, Ji Heon, Shim, Youn Hee, Nam, Trong Nguyen, Nguyen, Min-Ho, Lee, Dae Young, Lee, Tong Ho, Kang, Nam-In, Baek

المصدر: Molecules

مصطلحات موضوعية: animal structures, Neomycin, Cnidium officinale, Silicon Dioxide, zebrafish, Cnidium, Article, circular dichroism, stereostructure, embryonic structures, Hair Cells, Auditory, otic hair cells, Animals, phthalide, sense organs, Glycosides, ligusticoside, Rhizome, Benzofurans

الوصف: The extract from Cnidium officinale rhizomes was shown in a prior experiment to markedly recover otic hair cells in zebrafish damaged by neomycin. The current study was brought about to identify the principal metabolite. Column chromatography using octadecyl SiO2 and SiO2 was performed to isolate the major metabolites from the active fraction. The chemical structures were resolved on the basis of spectroscopic data, including NMR, IR, MS, and circular dichroism (CD) data. The isolated phthalide glycosides were assessed for their recovery effect on damaged otic hair cells in neomycin-treated zebrafish. Three new phthalide glycosides were isolated, and their chemical structures, including stereochemical characteristics, were determined. Two glycosides (0.1 μM) showed a recovery effect (p < 0.01) on otic hair cells in zebrafish affected by neomycin ototoxicity. Repeated column chromatography led to the isolation of three new phthalide glycosides, named ligusticosides C (1), D (2), and E (3). Ligusticoside C and ligusticoside E recovered damaged otic hair cells in zebrafish.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::3d164af1807e980312f111887010dee5Test
https://pubmed.ncbi.nlm.nih.gov/34834125Test

3

Aurones and Flavonols from Coreopsis lanceolata L. Flowers and Their Anti-Oxidant, Pro-Inflammatory Inhibition Effects, and Recovery Effects on Alloxan-Induced Pancreatic Islets in Zebrafish

المؤلفون: Nam-In Baek, Tong Ho Kang, Dae-Ok Kim, Dae Young Lee, Seon Min Oh, Trong Nguyen Nguyen, Youn Hee Nam, Hyoung-Geun Kim, Min-Ho Lee, Young Sung Jung

المصدر: Molecules, Vol 26, Iss 6098, p 6098 (2021)
Molecules
Volume 26
Issue 20

مصطلحات موضوعية: Coreopsis lanceolata, Flavonoid, RAW 264.7, Anti-Inflammatory Agents, Pharmaceutical Science, Organic chemistry, Flowers, antioxidant capacity, High-performance liquid chromatography, Article, Antioxidants, Analytical Chemistry, Cell Line, chemistry.chemical_compound, Islets of Langerhans, Mice, Flavonols, QD241-441, Alloxan, Drug Discovery, Animals, Humans, heterocyclic compounds, flavonoid, Physical and Theoretical Chemistry, Benzofurans, chemistry.chemical_classification, Flavonoids, Chromatography, pancreatic islets, Plant Extracts, fungi, food and beverages, Biological activity, zebrafish, anti-inflammation, carbohydrates (lipids), Leptosidin, RAW 264.7 Cells, chemistry, Chemistry (miscellaneous), Isoquercetin, Molecular Medicine, Astragalin, Coreopsis, Reactive Oxygen Species

الوصف: (1) Background: Many flavonoids have been reported to exhibit pharmacological activity
a preparatory study confirmed that Coreopsis lanceolata flowers (CLFs) contained high flavonoid structure content
(2) Methods: CLFs were extracted in aqueous methanol (MeOH:H2O = 4:1) and fractionated into acetic ester (EtOAc), normal butanol (n-BuOH), and H2O fractions. Repeated column chromatographies for two fractions led to the isolation of two aurones and two flavonols
(3) Results: Four flavonoids were identified based on a variety of spectroscopic data analyses to be leptosidin (1), leptosin (2), isoquercetin (3), and astragalin (4), respectively. This is the first report for isolation of 2–4 from CLFs. High-performance liquid chromatography (HPLC) analysis determined the content levels of compounds 1–4 in the MeOH extract to be 2.8 ± 0.3 mg/g (1), 17.9 ± 0.9 mg/g (2), 3.0 ± 0.2 mg/g (3), and 10.9 ± 0.9 mg/g (4), respectively. All isolated compounds showed radical scavenging activities and recovery activities in Caco-2, RAW264.7, PC-12, and HepG2 cells against reactive oxygen species. MeOH extract, EtOAc fraction, and 1–3 suppressed NO formation in LPS-stimulated RAW 264.7 cells and decreased iNOS and COX-2 expression. Furthermore, all compounds recovered the pancreatic islets damaged by alloxan treatment in zebrafish
(4) Conclusions: The outcome proposes 1–4 to serve as components of CLFs in standardizing anti-oxidant, pro-inflammatory inhibition, and potential anti-diabetic agents.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa6b0c49c2dc8d18119c0ac655a01921Test
https://www.mdpi.com/1420-3049/26/20/6098Test

4

Syringoleosides A-H, Secoiridoids from

المؤلفون: Jung Eun, Gwag, Yeong-Geun, Lee, Hyoung-Geun, Kim, Dong-Sung, Lee, Dae Young, Lee, Nam-In, Baek

المصدر: Journal of natural products. 83(9)

مصطلحات موضوعية: Lipopolysaccharides, Magnetic Resonance Spectroscopy, Molecular Structure, Cell Survival, Plant Extracts, Nitric Oxide Synthase Type II, Flowers, Syringa, Nitric Oxide, Mice, RAW 264.7 Cells, Animals, Iridoids, Enzyme Inhibitors

الوصف: Repeated column chromatography of

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::1486c79f508345ddb38e06999706598bTest
https://pubmed.ncbi.nlm.nih.gov/32936639Test

5

Anti-Inflammatory Effect of Flavonoids from

المؤلفون: Hyoung-Geun, Kim, Davin, Jang, Young Sung, Jung, Hyun-Ji, Oh, Seon Min, Oh, Yeong-Geun, Lee, Se Chan, Kang, Dae-Ok, Kim, Dae Young, Lee, Nam-In, Baek

المصدر: Journal of microbiology and biotechnology. 30(2)

مصطلحات موضوعية: Flavonoids, Molecular Structure, Cell Survival, Plant Extracts, Macrophages, Brugmansia, Anti-Inflammatory Agents, Nitric Oxide Synthase Type II, Flowers, Nitric Oxide, Mice, RAW 264.7 Cells, Cyclooxygenase 2, Animals, Chromatography, High Pressure Liquid

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::b11101f97936378d3a8f110a3aa6b237Test
https://pubmed.ncbi.nlm.nih.gov/31986558Test

6

Steamed Ginger May Enhance Insulin Secretion through K

المؤلفون: Youn Hee, Nam, Bin Na, Hong, Isabel, Rodriguez, Min Seon, Park, Seo Yule, Jeong, Yeong-Geun, Lee, Ji Heon, Shim, Tamanna, Yasmin, Na Woo, Kim, Young Tae, Koo, Sang Hun, Lee, Dong-Hyun, Paik, Yong Joon, Jeong, Hyelin, Jeon, Se Chan, Kang, Nam-In, Baek, Tong Ho, Kang

المصدر: Nutrients

الوصف: Ginger (Zingiber officinale Roscoe) and its active compounds (gingerols, shogaols and paradols) have been reported as having beneficial functions for several diseases, including diabetes. In this study, we revealed that the steaming process could enhance the anti-diabetic potential of ginger. To confirm the anti-diabetic effect of steamed ginger extract (GG03), we assessed pancreatic islets impaired by alloxan in zebrafish and demonstrated anti-hyperglycemic efficacy in a mouse model. The EC50 values of ginger extract (GE) and GG03 showed that the efficacy of GG03 was greater than that of GE. In addition, LC50 values demonstrated that GG03 had lower toxicity than GE, and the comparison of the Therapeutic Index (TI) proved that GG03 is a safer functional food. Furthermore, our data showed that GG03 significantly lowered hyperglycemia in a diabetic mouse model. HPLC was performed to confirm the change in the composition of steamed ginger. Interestingly, GG03 showed a 375% increase in 1-dehydro-6-gingerdione (GD) compared with GE. GD has not yet been studied much pharmacologically. Thus, we identified the protective effects of GD in the damaged pancreatic islets of diabetic zebrafish. We further assessed whether the anti-diabetic mechanism of action of GG03 and GD involves insulin secretion. Our results suggest that GG03 and GD might stimulate insulin secretion by the closure of KATP channels in pancreatic β-cells.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::766779916eff48796161670f3c9eb1b8Test
https://pubmed.ncbi.nlm.nih.gov/31991895Test

7

Brassicaphenanthrene A from Brassica�rapa protects HT22 neuronal cells through the regulation of Nrf2‑mediated heme oxygenase‑1 expression

المؤلفون: Anisuzzaman Chowdhury, Dong-Sung Lee, Bin Li, Hyuncheol Oh, Youn-Chul Kim, Sam Cheol Kim, Hwan Lee, Nam-In Baek, Eun Rhan Woo, Wonmin Ko

المصدر: Molecular Medicine Reports.

الوصف: Brain cell damage that results from oxidative toxicity contributes to neuronal degeneration. The transcription factor nuclear factor‑E2‑related factor 2 (Nrf2) regulates the expression of heme oxygenase (HO)‑1 and glutathione (GSH), and serves a key role in the pathogenesis of neurological diseases. Brassica rapa is a turnip that is unique to Ganghwa County, and is used mainly for making kimchi, a traditional Korean food. In the current study, brassicaphenanthrene A (BrPA) from B. rapa was demonstrated to exhibit protective effects against neurotoxicity induced by glutamate via Nrf2‑mediated HO‑1 expression. Similarly, BrPA increased the expression of cellular glutathione and glutamine‑cysteine ligase genes. Furthermore, BrPA caused the nuclear translocation of Nrf2 and increased antioxidant response element (ARE) promoter activity. Nrf2 also mediated HO‑1 induction by BrPA through the PI3K/Akt and JNK regulatory pathways. The results of the present study indicated the neuroprotective effect of BrPA, a natural food component from B. rapa.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b4f76a38048306989833b70b168f481Test
https://doi.org/10.3892/mmr.2019.10824Test

8

Anti-Inflammatory Mechanisms of Koreanaside A, a Lignan Isolated from the Flower of Forsythia koreana, against LPS-Induced Macrophage Activation and DSS-Induced Colitis Mice: The Crucial Role of AP-1, NF-κB, and JAK/STAT Signaling

المؤلفون: Kyung-Tae Lee, Nam-In Baek, Kyung-Sook Chung, Tae Woo Kim, Yeong-Geun Lee, Ji-Sun Shin

المصدر: Cells
Volume 8
Issue 10
Cells, Vol 8, Iss 10, p 1163 (2019)

مصطلحات موضوعية: 0301 basic medicine, Lipopolysaccharides, Male, Anti-Inflammatory Agents, Inflammatory bowel disease, NF-κB, chemistry.chemical_compound, Mice, 0302 clinical medicine, RAW 264.7 macrophages, STAT1, Glycosides, lcsh:QH301-705.5, DSS-induced colitis, Janus kinase 1, biology, Dextran Sulfate, NF-kappa B, JAK-STAT signaling pathway, General Medicine, Colitis, Nitric oxide synthase, STAT Transcription Factors, 030220 oncology & carcinogenesis, Tumor necrosis factor alpha, Signal Transduction, Flowers, Article, Lignans, Nitric oxide, 03 medical and health sciences, medicine, Animals, KA, Forsythia, Plant Extracts, Janus Kinase 1, Macrophage Activation, medicine.disease, AP-1, JAK/STAT, Mice, Inbred C57BL, Transcription Factor AP-1, 030104 developmental biology, RAW 264.7 Cells, lcsh:Biology (General), chemistry, Gene Expression Regulation, Cancer research, biology.protein

الوصف: The current treatment options for inflammatory bowel disease (IBD) are unsatisfactory. Therefore, novel and safer therapies are needed. We previously reported that koreanaside A (KA) showed high radical scavenging activity and suppressed vascular cell adhesion molecule 1 (VCAM-1) expression in vascular smooth muscle cells. However, the molecular mechanisms involved in its anti-inflammatory effect have not been reported. KA inhibited pro-inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nitric oxide (NO), and prostaglandin E2 (PGE2). KA inhibited the production and mRNA expression of interleukin (IL)-6 and tumor necrosis factor-&alpha
(TNF-&alpha
) induced by LPS. KA downregulated the myeloid differentiation primary response 88 (MyD88)-dependent inflammatory gene expressions in the MyD88-overexpressed cells. KA suppressed the LPS-induced transcriptional and DNA-binding activities of activator protein-1 (AP-1) and nuclear factor-kappa B (NF-&kappa
B). KA was found to inhibit the phosphorylation of Janus kinase 1/2 (JAK1/2) and signal transducers and activators of transcription 1/3 (STAT1/3). In DSS-induced colitis mice, KA relieved the symptoms of colitis by suppressing inflammatory cell infiltration, restoring tight junction (TJ)- and epithelial&ndash
mesenchymal transition (EMT)-related protein expression, and inactivating AP-1, NF-&kappa
B, and STAT1/3. Therefore, KA reduced inflammatory responses by downregulating AP-1, NF-&kappa
B, and JAK/STAT signaling in LPS-induced macrophages and DSS-induced colitis mice.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::648324e0635947808aa0342b834d0e5fTest
http://europepmc.org/articles/PMC6829247Test

9

Dehydrocorydaline promotes myogenic differentiation via p38 MAPK activation

المؤلفون: Jong-Sun Kang, Yong Kee Kim, Miran Yoo, Dong-Wan Seo, Sang-Jin Lee, Jae-Ha Ryu, Gyu-Un Bae, Nam-In Baek

المصدر: Molecular Medicine Reports

الوصف: Muscle regeneration is a coordinated process that involves proliferation and differentiation of muscle progenitor cells. Activation of MyoD is a key event in myogenic differentiation, which is regulated by p38 mitogen-activated protein kinases (MAPK). In a screen of natural compounds for the enhancement of MyoD activity, dehydrocorydaline (DHC) from the Corydalis tuber was identified. Treatment of C2C12 myoblasts with DHC increased the expression levels of muscle-specific proteins, including MyoD, myogenin and myosin heavy chain. In addition, C2C12 myoblasts exhibited enhanced multinucleated myotube formation without any cytotoxicity. Treatment with DHC elevated p38 MAPK activation and the interaction of MyoD with an E protein, which is likely to result in activation of MyoD and promotion of myoblast differentiation. Furthermore, defects in differentiation-induced p38 MAPK activation and myoblast differentiation induced by depletion of the promyogenic receptor protein Cdo in C2C12 myoblasts were restored by DHC treatment. In conclusion, these results indicated that DHC stimulates p38 MAPK activation, which can enhance heterodimerization of MyoD and E proteins, thus resulting in MyoD activation and myoblast differentiation. These findings suggested that DHC may be considered a potential therapeutic compound for the improvement of muscle stem cell regenerative capacity in injured muscle.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3be3042c43bfa77c100169e31a0481faTest
http://europepmc.org/articles/PMC5042734Test

10

Flavonoids from

المؤلفون: Yeong-Geun, Lee, Hwan, Lee, Jae-Woo, Jung, Kyeong-Hwa, Seo, Dae Young, Lee, Hyoung-Geun, Kim, Jung-Hwan, Ko, Dong-Sung, Lee, Nam-In, Baek

المصدر: International Journal of Molecular Sciences

مصطلحات موضوعية: Flavonoids, Lipopolysaccharides, Cell Survival, Oleaceae, HO-1, food and beverages, Glutamic Acid, Flowers, Article, Chionanthus retusus, flower, NO, Mice, RAW 264.7 Cells, Cell Line, Tumor, Animals, flavonoid, neuroprotection, Heme Oxygenase-1, Signal Transduction

الوصف: The dried flowers of Chionanthus retusus were extracted with 80% MeOH, and the concentrate was divided into EtOAc, n-BuOH, and H2O fractions. Repeated SiO2, octadecyl SiO2 (ODS), and Sephadex LH-20 column chromatography of the EtOAc fraction led to the isolation of four flavonols (1–4), three flavones (5–7), four flavanonols (8–11), and one flavanone (12), which were identified based on extensive analysis of various spectroscopic data. Flavonoids 4–6 and 8–11 were isolated from the flowers of C. retusus for the first time in this study. Flavonoids 1, 2, 5, 6, 8, and 10–12 significantly inhibited NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) and glutamate-induced cell toxicity and effectively increased HO-1 protein expression in mouse hippocampal HT22 cells. Flavonoids with significant neuroprotective activity were also found to recover oxidative-stress-induced cell damage by increasing HO-1 protein expression. This article demonstrates that flavonoids from C. retusus flowers have significant potential as therapeutic materials in inflammation and neurodisease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::342e67a81a7bea036ac2e45d356fe24bTest
https://pubmed.ncbi.nlm.nih.gov/31323752Test

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