دورية أكاديمية
B Lymphocytes Are Required during the Early Priming of CD4 + T Cells for Clearance of Pneumocystis Infection in Mice
| العنوان: | B Lymphocytes Are Required during the Early Priming of CD4 + T Cells for Clearance of Pneumocystis Infection in Mice |
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| المؤلفون: | Opata, Michael M., Hollifield, Melissa L., Lund, Frances E., Randall, Troy D., Dunn, Robert, Garvy, Beth A., Feola, David J. |
| المصدر: | Microbiology, Immunology, and Molecular Genetics Faculty Publications |
| بيانات النشر: | UKnowledge |
| سنة النشر: | 2015 |
| المجموعة: | University of Kentucky: UKnowledge |
| مصطلحات موضوعية: | Adoptive Transfer, Animals, Antibodies, Fungal, B-Lymphocytes, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Proliferation, Gene Deletion, Gene Expression, Homeodomain Proteins, Immunity, Humoral, Immunologic Memory, Lymphocyte Activation, Lymphocyte Depletion, Mice, Inbred BALB C, Inbred C57BL, Knockout, Pneumocystis, Pneumonia, Medical Immunology |
| الوصف: | B cells play a critical role in the clearance of Pneumocystis. In addition to production of Pneumocystis-specific Abs, B cells are required during the priming phase for CD4+ T cells to expand normally and generate memory. Clearance of Pneumocystis was found to be dependent on Ag specific B cells and on the ability of B cells to secrete Pneumocystis-specific Ab, as mice with B cells defective in these functions or with a restricted BCR were unable to control Pneumocystis infection. Because Pneumocystis-specific antiserum was only able to partially protect B cell–deficient mice from infection, we hypothesized that optimal T cell priming requires fully functional B cells. Using adoptive transfer and B cell depletion strategies, we determined that optimal priming of CD4+ T cells requires B cells during the first 2–3 d of infection and that this was independent of the production of Ab. T cells that were removed from Pneumocystis-infected mice during the priming phase were fully functional and able to clear Pneumocystis infection upon adoptive transfer into Rag1−/− hosts, but this effect was ablated in mice that lacked fully functional B cells. Our results indicate that T cell priming requires a complete environment of Ag presentation and activation signals to become fully functional in this model of Pneumocystis infection. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | The online version of this article contains supplemental material .; https://uknowledge.uky.edu/microbio_facpub/145Test; https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1144&context=microbio_facpubTest |
| الإتاحة: | https://uknowledge.uky.edu/microbio_facpub/145Test https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1144&context=microbio_facpubTest |
| رقم الانضمام: | edsbas.2A762998 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |