Erratum to 'Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC)' [Vet. Microbiol. 202 (2017) 79–89]
| العنوان: | Erratum to 'Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC)' [Vet. Microbiol. 202 (2017) 79–89] |
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| المؤلفون: | Weiping Zhang, Qiangde Duan, Dana Rausch, Thomas A. Casey, Rahul M. Nandre, Xiaosai Ruan, Emad A. Hashish |
| المصدر: | Veterinary Microbiology |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Antitoxin vaccine, Toxoid multiepitope fusion antigen (MEFA), Swine, Bacterial Toxins, medicine.disease_cause, digestive system, Shiga Toxin 2, Microbiology, Article, Enterotoxins, fluids and secretions, Antigen, Enterotoxigenic Escherichia coli, medicine, Animals, Pig diarrhea, Antigens, Bacterial, General Veterinary, biology, Toxin, Escherichia coli Proteins, Toxoid, Shiga toxin, General Medicine, Toxoids, Heat labile, bacterial infections and mycoses, Antibodies, Bacterial, Antibodies, Neutralizing, Enterotoxigenic Escherichia coli (ETEC), Recombinant Proteins, biology.protein, Erratum, Antibody |
| الوصف: | Highlights • ETEC toxin MEFAs (multiepitope fusion antigen) were constructed. • Toxin MEFAs induce neutralizing antibodies to all four porcine ETEC toxins. • Passive maternal antibodies derived from the toxoid MEFA protect suckling piglets against ETEC diarrhea. • Toxin MEFA can be used for vaccines against porcine ETEC diarrhea. Enterotoxigenic Escherichia coli (ETEC) strains are the main cause of diarrhea in pigs. Pig diarrhea especially post-weaning diarrhea remains one of the most important swine diseases. ETEC bacterial fimbriae including K88, F18, 987P, K99 and F41 promote bacterial attachment to intestinal epithelial cells and facilitate ETEC colonization in pig small intestine. ETEC enterotoxins including heat-labile toxin (LT) and heat-stable toxins type Ia (porcine-type STa) and type II (STb) stimulate fluid hyper-secretion, leading to watery diarrhea. Blocking bacteria colonization and/or neutralizing enterotoxicity of ETEC toxins are considered effective prevention against ETEC diarrhea. In this study, we applied the MEFA (multiepitope fusion antigen) strategy to create toxoid MEFAs that carried antigenic elements of ETEC toxins, and examined for broad antitoxin immunogenicity in a murine model. By embedding STa toxoid STaP12F (NTFYCCELCCNFACAGCY), a STb epitope (KKDLCEHY), and an epitope of Stx2e A subunit (QSYVSSLN) into the A1 peptide of a monomeric LT toxoid (LTR192G), two toxoid MEFAs, ‘LTR192G-STb-Stx2e-STaP12F’ and ‘LTR192G-STb-Stx2e-3xSTaP12F’ which carried three copies of STaP12F, were constructed. Mice intraperitoneally immunized with each toxoid MEFA developed IgG antibodies to all four toxins. Induced antibodies showed in vitro neutralizing activities against LT, STa, STb and Stx2e toxins. Moreover, suckling piglets born by a gilt immunized with ‘LTR192G-STb-Stx2e-3xSTaP12F’ were protected when challenged with ETEC strains, whereas piglets born by a control gilt developed diarrhea. Results from this study showed that the toxoid MEFA induced broadly antitoxin antibodies, and suggested potential application of the toxoid MEFA for developing a broad-spectrum vaccine against ETEC diarrhea in pigs. |
| تدمد: | 0378-1135 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7baf5bb0a24ecf1fb6174027346b83faTest https://doi.org/10.1016/j.vetmic.2018.04.034Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7baf5bb0a24ecf1fb6174027346b83fa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03781135 |
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